Chromoblastomycosis murine model and in vitro test to evaluate the sensitivity of Fonsecaea pedrosoi to ketoconazole, itraconazole and saperconazole

Cardona-Castro, Nora; Agudelo‐Flórez, Piedad; Restrepo-Molina, Rodrigo

doi:10.1590/s0074-02761996000600026

Cited by 16 publications

(13 citation statements)

References 27 publications

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“…18,19,25,26 However, inoculation of viable cells from a CBM agent at different peritoneal sites in mice allows increased fungal persistence. [27][28][29] Following studies that showed increased host susceptibility to F. pedrosoi, we have become interested in inflammatory response at one infectious site (footpad) depends on the elimination of the antigenic stimulus at another site (peritoneum). Probably, the simultaneous immune response to specific pathogens at multiple sites resolves the infection only one site at a time.…”

Section: Resultsmentioning

confidence: 99%

“…9 A type of chronic F. pedrosoi infection occurs following fungal inoculation at various peritoneal sites. 27,28 Similarly, we observed that BALB/c mice stimulated at two sites (peritoneum and footpad) with viable F. pedrosoi cells developed prolonged infection. Further experiments have shown that this long course of infection has also arisen when animals were inoculated with viable and nonviable cells at the footpad (s.c.) and peritoneum (i.p.…”

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confidence: 75%

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Prolonged infection byFonsecaea pedrosoiafter antigenic co-stimulation at different sites in experimental murine chromoblastomycosis

2010

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Section: Resultsmentioning

confidence: 99%

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confidence: 75%

Prolonged infection byFonsecaea pedrosoiafter antigenic co-stimulation at different sites in experimental murine chromoblastomycosis

2010

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“…There are few literature reports on antifungal susceptibility of dematiaceous fungi. [21][22][23][24] Considering the emergence of resistance and the adverse effects of the available antifungal drugs, there is a need for new pharmacological agents. An intense selective pressure on plants exerted by pathogenic fungi allows for the development of a wide range of phytochemicals with antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity ofPterocaulon alopecuroides(Asteraceae) against chromoblastomycosis agents

Daboit

Stopiglia

Poser

et al. 2010

Mycoses

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Plants of the genus Pterocaulon (Asteraceae) are popularly used in the treatment of skin diseases caused by fungi and bacteria. The aim of this work was to investigate the in vitro activity of the crude methanolic extract obtained from the aerial parts of Pterocaulon alopecuroides (Lam.) against some agents of chromoblastomycosis, a chronic fungal infection of the skin and of the subcutaneous tissue caused by traumatic inoculation of the aetiological agent. The extract was active against all the strains tested showing a minimum inhibitory concentration between 625 and 2500 microg ml(-1). The assessment of fungistatic/fungicidal activity demonstrated that the extract was fungistatic against Fonsecaea spp. and fungicidal against all the other fungi. Our results indicate that the identification of bioactive components present in the crude methanolic extract of P. alopecuroides against chromoblastomycosis agents can be an important strategy to manage this mycosis in the future.

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“…Fonsecaea pedrosoi inoculated in various peritoneal sites caused persistent infection (18,27). Suppressed host immune response in the presence of chromoblastomycotic agents may be related to the induction of a specific population of T cells (4).…”

Section: Discussionmentioning

confidence: 99%

Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt

Machado¹,

Freymüller²,

Fischman³

2009

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10 4 cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in one footpad, whereas in the other group, both footpads were infected. Active lesions were observed up to seven months post-infection, particularly in mice inoculated at two sites. After this period, animals were killed. Histological sections revealed characteristics bearing a strong resemblance to the human form of the disease such as tissue hyperplasia, granulomas with microabscesses and sclerotic cells. Based on this study, we identified fungal cells from old cultures capable of provoking chronic chromoblastomycosis under experimental conditions, especially when more than one site is infected.

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Chromoblastomycosis murine model and in vitro test to evaluate the sensitivity of Fonsecaea pedrosoi to ketoconazole, itraconazole and saperconazole

Abstract: An experimental model of murine chromoblastomycosis and in vitro tests with

Cited by 16 publications

References 27 publications

Prolonged infection byFonsecaea pedrosoiafter antigenic co-stimulation at different sites in experimental murine chromoblastomycosis

Prolonged infection byFonsecaea pedrosoiafter antigenic co-stimulation at different sites in experimental murine chromoblastomycosis

Antifungal activity ofPterocaulon alopecuroides(Asteraceae) against chromoblastomycosis agents

Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt

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