Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens

Genç, Osman; Aksu, Evrim

doi:10.1016/j.mimet.2018.04.014

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…Recently, Genc et al performed a comparative study including 100 K. pneumonia isolates assessed with the OXA-48 K-Set test and four chromogenic media (CHROMagar KPC, CHROMagar mSuperCARBA, ChromID Carba and ChromID OXA-48). The authors found that 60 of the isolates were positive for OXA-48, and reported that the sensitivities and specificities of all chromogenic media (and the rapid test) were 100% [9]. In accordance with these studies, we found that the ChromID OXA-48 media had a sensitivity of 100% and a specificity of 88.8% for detection of OXA-48 positive K. pneumoniae isolates.…”

Section: Discussionsupporting

confidence: 86%

“…Standard detection of OXA-48 is based on molecular techniques, mostly polymerase chain reaction (PCR), which is costly and difficult to apply for many laboratories [7]. Different non-molecular techniques have been used to detect carbapenem resistant bacteria, including phenotypic tests (combination disk test, gradient diffusion strips, double disk synergy test), colorimetric tests based on carbapenem hydrolysis (cloverleaf method, colorimetric assays, Carba NP, Blue-CARBA, carbapenem inhibition, starch-iodine assay) and immunochromatographic methods [8,9]. However, the results of studies evaluating OXA-48-producing bacteria using non-molecular techniques are inconclusive and limited.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

Sanmak

Aksaray

2021

J CLIN EXP INVEST

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Objectives:The study was aimed to compare the performance of three different approaches (temocillin disc diffusion test (TDD), immunochromatographic assay, OXA-48 K-SET, and chromogenic assay containing ChromID OXA-48 media) with PCR in a collection of K. pneumoniae producing OXA-48. Materials and methods:A total of 45 carbapenem resistant K. pneumoniae isolates (27 were OXA-48 positive) were included in the study. Polymerase chain reaction (PCR), TDD, OXA-48 K-SET, ChromID OXA-48 media were applied to all isolates. Results:The OXA-48 K-SET was found to be 100% compatible with gold standard PCR for the identification of OXA-48 positive Klebsiella pneumoniae strains. 100% sensitivity and 88.8% specificity were found with the ChromID OXA-48 medium method. Sensitivity and specificity of the TDD assay were found 100% and 77.8%, respectively. Conclusion:The OXA-48 K-Set had excellent performance for detecting OXA-48 carbapenemase and can be used easily and reliably in the routine clinical microbiology laboratory for rapid detection of OXA-48 producing K. pneumoniae isolates, particularly in endemic regions. ChromID media and temocillin disc diffusion testing can be applied in laboratories as part of the identification and screening of carbapenemase-positive Klebsiella pneumoniae.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

Sanmak

Aksaray

2021

J CLIN EXP INVEST

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“…while Xpert Carba-R was more accurate and faster [17]. In detection of KPC-2 and VIM-1-producing K. pneumoniae isolates, the positive predictive values (PPV) and negative predictive values (NPV) of CHROMagar KPC for the detection of CRE was 100% and 98.8%, respectively [18]. To detect OXA-48like producers, reported that the sensitivity and specificity of CHROMagar for detection of carbapenemase-producing isolates was 88.5% and 86.1% [19].…”

Section: Introductionmentioning

confidence: 97%

Clinical Laboratory Aspect of Carbapenem-Resistant Enterobacteriaceae

Park

2020

Korean J Clin Lab Sci.

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The correct distinction of carbapenem-resistant Enterobacteriaceae (CRE) and ccarbapenemase producing Enterobacteriaceae (CPE) and the rapid detection of CPE are important for instituting the correct treatment and management of clinical infections. Screening protocols are mainly based on cultures of rectal swab specimens on selective media followed by phenotypic tests to confirm a carbapenem-hydrolyzing activity, the rapid carbapenem inactivation method, lateral flow immunoassay, the matrix-assisted laser desorption ionization-time-of-flight test and molecular methods. The CPE is accurate for detection, and is essential for the clinical treatment and prevention of infections. A variety of phenotypic methods and gene-based methods are available for the rapid detection of carbapenemases, and these are expected to be routinely used in clinical microbiology laboratories. Therefore, to control the spread of carbapenemase, many laboratories around the world will need to use reliable, fast, high efficiency, simple and low cost methods. Optimal effects in patient applications would require rapid testing of CRE to provide reproducible support for antimicrobial management interventions or the treatment by various types of clinicians. For the optimal test method, it is necessary to combine complementary test methods to discriminate between various resistant bacterial species and to discover the genetic diversity of various types of carbapenemase for arriving at the best infection control strategy.

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“…These patients had a prior surveillance or clinical Klebsiella pneumoniae culture within 7 days of enrollment in a study design similar to that described by O'Hara et al (1). Each swab was cultured overnight on CHROMagar KPC to identify K. pneumoniae and other Klebsiella species (2). Antibiotic susceptibility testing was performed for all single-colony selected isolates by disk diffusion and interpreted in accordance with CLSI guidelines (3).…”

mentioning

confidence: 99%

Draft Genome Sequences of Five Diverse Klebsiella Species Isolates from Intensive Care Unit Patients

Hazen

Hitchcock

O’Hara

et al. 2020

Microbiol Resour Announc

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Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens

Cited by 9 publications

References 19 publications

Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

Clinical Laboratory Aspect of Carbapenem-Resistant Enterobacteriaceae

Draft Genome Sequences of Five Diverse Klebsiella Species Isolates from Intensive Care Unit Patients

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