1980
DOI: 10.1016/0027-5107(80)90202-x
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Chromosomal aberrations in peripheral lymphocytes of alcoholics

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Cited by 73 publications
(12 citation statements)
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“…Amplification primers were the same as described previously (16). PCR products (5)(6)(7)(8)(9)(10) pl) were treated with alkali and transferred to nylon membranes, which were then baked and hybridized overnight with the 32P-ATP labeled probe (Amersham, Bucks, U.K.), for either ALDH2' (5'-GTTTTCACTTC AGTGTATG) or ALDH22 allele (5'-GTTTTCACTTTAGTGTATG). The membranes were washed three times at room temperature, washed with 1 x SSC (standard saline citrate) containing 0.1% sodium dodecyl sulfate (SDS) for 30 min at 48' C, and exposed to X-ray film overnight.…”
Section: Methodsmentioning
confidence: 99%
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“…Amplification primers were the same as described previously (16). PCR products (5)(6)(7)(8)(9)(10) pl) were treated with alkali and transferred to nylon membranes, which were then baked and hybridized overnight with the 32P-ATP labeled probe (Amersham, Bucks, U.K.), for either ALDH2' (5'-GTTTTCACTTC AGTGTATG) or ALDH22 allele (5'-GTTTTCACTTTAGTGTATG). The membranes were washed three times at room temperature, washed with 1 x SSC (standard saline citrate) containing 0.1% sodium dodecyl sulfate (SDS) for 30 min at 48' C, and exposed to X-ray film overnight.…”
Section: Methodsmentioning
confidence: 99%
“…This work was supported in part by of alcohol-related diseases (5)(6)(7)(8)(9)(10). Alcohol is metabolized to acetaldehyde, which directly causes various types of chromosomal DNA lesions and alcohol-related diseases; it is further detoxified to the less toxic metabolite acetate (11,12).…”
Section: Introductionmentioning
confidence: 99%
“…These genetic abnormalities occur due to previous exposure to mutagens like benzene, alkylating agents, gasoline, topoisomerase II inhibitors, radiation etc. [7][8][9][10][11][12][13]. Due to exposure to insecticides, pesticides and herbicides agricultural workers are reported to have higher incidence of developing MDS [7].…”
Section: Introductionmentioning
confidence: 99%
“…Several studies describe alcohol as a potential risk factor for development of MDS. Although the exact link between alcohol and MDS is not yet clear, it is reported that alcoholics induce chromosomal abnormalities in hematopoietic cells with altered gene expression patterns and adverse effects on the immune system [9,10,11]. Tobacco products like cigarettes which contain benzene and other carcinogens also induce chromosomal aberrations in primitive bone marrow cells leading to development of MDS [9,12,13].…”
Section: Introductionmentioning
confidence: 99%
“…In vivo, however, cytogenetic effects have been reported in several studies. Thus, elevated frequencies of exchange type aberrations (OBE and HERHA 1975;LILLY 1975;OBE et al 1980), as well as of chromosome breaks (MITELMAN and WADSTEIN 1978) have been found. No increase in sister chromatid exchange (SCE) rates has been found after in vitro treatment with ethanol of Chinese hamster ovary cells , nor of human lymphocytes .…”
mentioning
confidence: 97%