Chromosomal Damage by Alcohol In Vitro and In Vivo

Obe, G.; Hj, Ristow; Herha, J.

doi:10.1007/978-1-4899-5181-6_4

Cited by 29 publications

(9 citation statements)

References 48 publications

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“…Amplification primers were the same as described previously (16). PCR products (5)(6)(7)(8)(9)(10) pl) were treated with alkali and transferred to nylon membranes, which were then baked and hybridized overnight with the 32P-ATP labeled probe (Amersham, Bucks, U.K.), for either ALDH2' (5'-GTTTTCACTTC AGTGTATG) or ALDH22 allele (5'-GTTTTCACTTTAGTGTATG). The membranes were washed three times at room temperature, washed with 1 x SSC (standard saline citrate) containing 0.1% sodium dodecyl sulfate (SDS) for 30 min at 48' C, and exposed to X-ray film overnight.…”

Section: Methodsmentioning

confidence: 99%

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Low Km aldehyde dehydrogenase (ALDH2) polymorphism, alcohol-drinking behavior, and chromosome alterations in peripheral lymphocytes.

Morimoto

Takeshita

1996

Environ Health Perspect

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Excessive drinking of alcohol is now widely known to be one of the major lifestyle choices that can affect health. Among the various effects of alcohol drinking, cytogenetic and other genotoxic effects are of major concern from the viewpoint of prevention of alcohol-related diseases. Alcohol is first metabolized to acetaldehyde, which directly causes various types of chromosomal DNA lesions and alcohol-related diseases, and is then further detoxified to the much less toxic metabolite acetate. About 50% of Oriental people are deficient in the aldehyde-dehydrogenase 2 isozyme (ALDH2) that can most efficiently detoxify acetaldehyde. We have performed a series of experiments to investigate how the genetic deficiency in ALDH2 affects the behavioral pattern for alcohol drinking and the sensitivity of peripheral lymphocytes to the induction of chromosome alterations by exposure to alcohol and alcohol-related chemicals. We found great effects of the ALDH2 genotypes on alcohol sensitivity and alcohol-drinking behavior. We also show that lymphocytes from habitual drinkers with the deficient ALDH2 enzyme had significantly higher frequencies of sister chromatid exchanges than those from ALDH2-proficient individuals.

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Section: Methodsmentioning

confidence: 99%

“…This work was supported in part by of alcohol-related diseases (5)(6)(7)(8)(9)(10). Alcohol is metabolized to acetaldehyde, which directly causes various types of chromosomal DNA lesions and alcohol-related diseases; it is further detoxified to the less toxic metabolite acetate (11,12).…”

Section: Introductionmentioning

confidence: 99%

Low Km aldehyde dehydrogenase (ALDH2) polymorphism, alcohol-drinking behavior, and chromosome alterations in peripheral lymphocytes.

Morimoto

Takeshita

1996

Environ Health Perspect

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“…In einem bakteriellen Mutagenitätstest mit Salmonella typhimurium war 1-Pentanol negativ, genauere Angaben zu dieser Untersuchung fehlen aber (Obe et al 1977). In einem Abstract wird berichtet, dass sich 3-Methyl-1-butanol in einem Spot-Test mit den Salmonella-Stämmen TA98, TA100, TA1535 und TA1537 in An-und Abwesenheit eines metabolischen Aktivierungssystems als toxisch erwies (k. w.…”

Section: In Vitrounclassified

“…Leukozyten wurden 24 Stunden lang mit 0,2 Volumenprozent 1-Pentanol inkubiert, 48 Stunden lang kultiviert und nach 4,5-stündiger Colcemid-Behandlung auf Chromosomenbrüche analysiert. 1-Pentanol führte nicht zu vermehrten Chromosomenbrüchen (Obe et al 1977). Ein HPRT-Test mit V79-Zellen war nach Gabe von bis zu 700 mg 3-Methyl-1-butanol/ml in An-und Abwesenheit eines metabolischen Aktivierungssystems negativ (Seidel und Plappert 1999).…”

Section: In Vitrounclassified

“…0,2 Volumenprozent 3-Methyl-1-butanol führten zu einer 37%igen, 0,6 Volumenprozent 3-Methyl-1-butanol zu einer 86,5%igen Hemmung der RNASynthese, die jeweils als Trichloressigsäure-Niederschlag gemessen wurden. Bei 2-Methyl-1-pentanol betrug die Hemmung 23,5% bei 0,2 Volumenprozent und 48% bei 0,6 Volumenprozent (Obe et al 1977). Dies zeigt einen Einfluss dieser Pentanol-Isomeren auf den Stoffwechsel.…”

Section: Pentanol-isomeren 23unclassified

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Pentanol‐Isomeren [MAK Value Documentation in German language, 2008]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 44. Lieferung, Ausgabe 2008 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Aufnahme, Verteilung, Ausscheidung Metabolismus Erfahrungen beim Menschen Einmalige Exposition Wiederholte Exposition Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Sonstige Wirkungen Bewertung Anhang

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Impact of dried, creamed and cake supply formats on the genetic variation and ethanol tolerance of three Saccharomyces cerevisiae distilling strains

et al. 2012

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Scotch whisky fermentations typically employ high-gravity fermentation practices to maximize product formation and to minimize both energy and water inputs. This approach increases ethanol concentrations at the end of fermentation, creating stressful conditions for the yeast. In this work we examined the relative tolerance of four Saccharomyces cerevisiae distilling yeast strains, supplied in dried, creamed, cake or slurry format, to ethanol under CO 2 -induced anaerobic conditions. The cells were assessed for their capacity to recover and grow on inhibition spot plates and to maintain cell viability in ethanol-dosed suspensions. Variations in ethanol tolerance were observed between strains and between the same strain supplied in different formats. The creamed yeast format typically exhibited a higher tolerance to ethanol. One possible explanation for this observation is that cells surviving the dehydration and rehydration process might incur sub-lethal genome damage. Thus the genetic integrity of the most ethanol-tolerant strain was assessed as a function of supply format (two dried and one creamed). The mitochondrial DNA was examined using mitochondrial restriction fragment length polymorphism and the chromosomal DNA using pulsed field gel electrophoresis and polymerase chain reaction with both ITS and delta-specific primers. In one dried yeast sample, genetic integrity was compromised, highlighting the requirement for yeast intake quality assurance programmes.

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Chromosomal Damage by Alcohol In Vitro and In Vivo

Cited by 29 publications

References 48 publications

Low Km aldehyde dehydrogenase (ALDH2) polymorphism, alcohol-drinking behavior, and chromosome alterations in peripheral lymphocytes.

Low Km aldehyde dehydrogenase (ALDH2) polymorphism, alcohol-drinking behavior, and chromosome alterations in peripheral lymphocytes.

Pentanol‐Isomeren [MAK Value Documentation in German language, 2008]

Impact of dried, creamed and cake supply formats on the genetic variation and ethanol tolerance of three Saccharomyces cerevisiae distilling strains

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