Chromosomal genotypes (evolutionary lines) of<i>Salmonella berta</i>

Stanley, John R.; Chowdry, N.; Powell, Neville; Threlfall, E. J.

doi:10.1111/j.1574-6968.1992.tb05374.x

Cited by 17 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on isoenzyme analysis of 23 enzymes, isolates of S. typhimurium show approximately 80% similarity (Beltran et al, 1988), but these authors did not report the phage type distribution, which makes comparison to the present investigation impossible. Compared with other serotypes of S. enterica, S. typhimurium seems to display a greater degree of diversity as indicated by both isoenzyme analysis (Beltran et al, 1988), and by the large span in IS200 copy number relative to copy number variation in other important serotypes (see Stanley et al, 1991Stanley et al, ,1992.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic relationships between selected phage types of Salmonella enterica subsp. enterica serotype typhimurium defined by ribotyping, IS200 typing and PFGE

et al. 1997

View full text Add to dashboard Cite

The genomic relationship between isolates representing 17 definitive phage types (DTs) of Salmonella enterica subsp. enterica serotype typhimurium (5. typhimurium) were analysed using three different typing methods: IS200 typing using the restriction enzymes €coRI and Pvull, ribotyping using SmaI and EcoRI, and PFGE using Xbal. These methods were used to study four DTs in greater detail; in all 18 (DT 49), 10 (DT l l O ) , five (DT 120) and seven (DT 135) isolates were studied. The combined data generated two large clusters, which could be divided into five groups. Within the first cluster, a close similarity was indicated between isolates of the following phage types: group A -DTs 44,49, 135 and 204c, with DT 9 distantly related; group B -DTs 95 and 99; and group C -DTs 104a, 110 and 120. The other large cluster contained group D -DTs 10, 20 and 146, with DT 12 distantly related, and group E -DTs 69,103 and 153. The same grouping was observed by principal component analysis, but a minimum spanning tree linked DT 12 to group E and not group D in this analysis. Among the typing methods used, IS200 gave the best representation of the overall similarity between the 5. typhimurium isolates. Five different IS200 profiles were obtained among isolates belonging to DT 49. Only one profile was observed within each of the phage types DT 110,120 and 135. All isolates within each of these four phage types were of one ribotype. Isolates of DT 49 showed four PFGE patterns, while one pattern was present within isolates of the three other phage types. Members of these four phage types were found to be clonally related as they formed tight subclusters separated from isolates of other phage types.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Isolates with the same copy number are assumed to share evolutionary traits, especially when the insertion elements are located within DNA fragments of the same size (Stanley et al, 1991(Stanley et al, , 1992. Stanley et a/.…”

Section: Discussionmentioning

confidence: 99%

Genomic relationships between selected phage types of Salmonella enterica subsp. enterica serotype typhimurium defined by ribotyping, IS200 typing and PFGE

et al. 1997

View full text Add to dashboard Cite

show abstract

Use of ribotyping, IS200 typing and plasmid analysis for the identification of Salmonella enterica subsp.enterica serovar typhimurium vaccine strain Zoosaloral H and its differentiation from wild type strains of the same serovar

Schwarz

Liebisch

1994

Zentralblatt für Bakteriologie

View full text Add to dashboard Cite

Virulence and genotype stability ofSalmonella entericaserovar Berta during a natural outbreak

et al. 1996

View full text Add to dashboard Cite

SUMMARYStrains of Salmonella enterica serotype Berta, collected over a period of 6 years from a well documented natural outbreak in Denmark, have been characterized in order to assess the stability of chromosomal typing systems and virulence properties. Outbreak strains were identical in Pvu II and Pst I IS200 profiles, all but two strains showed the same Sma I ribotype, and all but one strain showed the same Not I pulsed field gel electrophoretic pattern, indicating that these molecular markers remained almost constant during the outbreak. In general, strains of S. Berta were found to be of moderate to low virulence; log VC10 values were found to vary between 3 0 and 4 4 after i.p. challenge of mice, and maximum CFU in internal organs of dayold chicks varied between 2 and 4 logl0 units following oral challenge. The minor differences observed between strains in vivo did not correlate with differences in in vitro invasion into cultured MDCK cells, nor with in vitro growth characteristics. A succession of different plasmid profile types was observed during the outbreak but a hierarchical selection of clones based on differences in virulence was unlikely to have caused the succession of types of S. Berta during this outbreak.

show abstract

Chromosomal genotypes (evolutionary lines) ofSalmonella berta

Cited by 17 publications

References 13 publications

Genomic relationships between selected phage types of Salmonella enterica subsp. enterica serotype typhimurium defined by ribotyping, IS200 typing and PFGE

Genomic relationships between selected phage types of Salmonella enterica subsp. enterica serotype typhimurium defined by ribotyping, IS200 typing and PFGE

Use of ribotyping, IS200 typing and plasmid analysis for the identification of Salmonella enterica subsp.enterica serovar typhimurium vaccine strain Zoosaloral H and its differentiation from wild type strains of the same serovar

Virulence and genotype stability ofSalmonella entericaserovar Berta during a natural outbreak

Contact Info

Product

Resources

About