Chromosomal heterochromatin differentiation in Salmo trutta with restriction enzymes

Sánchez, Laura; Martı́nez, Paulino; Bouza, Carmen; Viñas, Ana

doi:10.1038/hdy.1991.30

Cited by 20 publications

(10 citation statements)

References 28 publications

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“…Current results also show that NORs in the European huchen are built with heterochromatin enriched in the restriction sites recognized by Dde I and Alu I. Similar observations have been reported in other fish species (SANCHEZ et al 1991;SALVADORI et al 1997;JANKUN et al 1998;DI et al 2006). Additionally, similar interindividual size heteromorphism of the NOR-bearing chromosome short arms observed in the European huchen was also recognized in the Siberian taimen (FROLOV & FROLOVA 2000).…”

Section: Discussionsupporting

confidence: 88%

Distribution and Heterogeneity of Heterochromatin in the European Huchen (<I>Hucho hucho</I> Linnaeus, 1758) (Salmonidae)

Kuciński

Ocalewicz²,

Fopp‐Bayat

et al. 2014

folia biol (krakow)

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The chromosomal characteristics, locations and variations of the heterochromatin were studied in the European huchen (Hucho hucho, Linnaeus, 1758) karyotype using conventional C-banding, endonuclease digestion banding, silver nitrate (AgNO !), chromomycin A ! (CMA !) and DAPI staining techniques. The karyotype consists of 82 chromosomes: 13 pairs of metacentric chromosomes, 2 pairs of submetacentric chromosomes and 26 pairs of subtelo-acrocentric chromosomes (NF=112). Original data on the chromosomal distribution of segments resistant to Alu I, Dde I and Mbo I restriction endonucleases and identification of the C-banded heterochromatin presented here have been used to characterize the huchen karyotype. On the basis of the banding patterns provided in the course of restriction enzyme digestion, AgNO ! /CMA ! staining and C-banding we distinguished twelve types of heterochromatin grouped in four areas of the European huchen chromosomes. One pair of NOR-bearing chromosomes was found to be polymorphic in size and displayed two distinct forms. A combination of various staining techniques provided original data concerning heterochromatin distribution and heterogeneity in the European huchen which enabled better karyotypic definition of this fish species.

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Section: Discussionsupporting

confidence: 88%

Distribution and Heterogeneity of Heterochromatin in the European Huchen (<I>Hucho hucho</I> Linnaeus, 1758) (Salmonidae)

Kuciński

Ocalewicz²,

Fopp‐Bayat

et al. 2014

folia biol (krakow)

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“…2d). Some studies have been made to analyze the action of restriction enzymes on chromosomic preparations of fish, such as SANCHEZ et al, (1990SANCHEZ et al, ( , 1991, ABUIN et al (1994), BOUZA et al (1994), in which the use of the restriction enzyme Alu I also produced a linear differentiation similar to C banding, as observed in the species of fish analyzed here.…”

Section: Resultsmentioning

confidence: 84%

Cytogenetic characterization through chromosomic banding of Pinirampus pirinampu (Pisces, Pimelodidae) from the Tibagi river basin PR/Brazil

1999

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-Specimens of Pinirampus pirinampu were analyzed cytogenetically. Fifteen individuals were obtained from the Tibagi river near Sertaneja, PR, Brazil. A karyotypic struc ture consisting of 50 chromosomes distributed as 26M+12SM+2ST+10A was observed. The nucleolar organizing regions ( NORs) were identified on the short arm of a pair of subtelocentric chromosomes. A variation in size of the NOR regions was observed among the paired chromosomes. Chromomycin (CMA 3 ) staining established not only the nucleolar chromosome pair, but also fluorescent marking in the telomeric and centromeric regions of other chromosomes which seem to correspond to the distribution patterns of the constitutive heterochromatin. Restriction enzyme Alu I was employed and the reaction pattern obtained also corresponded to the heterochromatin constitutive distribution.

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“…Great difficulties have arisen in the C-and G-banding studies of amphibians, and only in the last few years data on the sensitivity of chromo somes to the various banding techniques have been reported in teleosteans (Lloyd and Thorgaard, 1988;Cau et al, 1988Cau et al, .1992 Lozano et al, 1991;Sanchez et al, 1991; Hartley, 1991a. b; Padilla et al, I993;Vinaset al, 1994).…”

mentioning

confidence: 99%

“…Data on tele osteans have accumulated, contributing to the elucidation of the role of heterochromatin components in evolutionary phe nomena (Gold et al. 1986;Sanchez et al, 1991). In view of this, we undertook a study of the effects of several RE, as well as of heterochromatin and NOR localization, in two cogeneric species belonging to the superorder Batoidea in order to get information on the localization of particular DNA sequences with different functional roles.…”

mentioning

confidence: 99%

NOR and heterochromatin analysis in two cartilaginous fishes by C-, Ag- and RE (restriction endonuclease)-banding

Stingo

Rocco²,

Odierna³

et al. 1995

Cytogenet Genome Res

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Selachian chromosomes were investigated for the first time by C-, Ag- and RE (restriction endonuclease)-band-ing. A pair of NOR-bearing acrocentric chromosomes, as well as a third extra site, were demonstrated in two species of Torpedo. In situ digestion with restriction endonucleases revealed a peculiar arrangement of constitutive heterochromatin: in Torpedo ocellata the centromere never appeared C-banded, and was always digested by AluI, PstI, DdeI and HaeIII; in Torpedo marmorata, instead, heterochromatin was present in the centromere, and was not digested by the same enzymes. The occurrence of different types of centromeric heterochromatin suggests an involvement of highly repeated DNA fractions in the Robertsonian phenomena that occurred in chromosome rearrangements in the genus Torpedo.

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Chromosomal heterochromatin differentiation in Salmo trutta with restriction enzymes

Cited by 20 publications

References 28 publications

Distribution and Heterogeneity of Heterochromatin in the European Huchen (<I>Hucho hucho</I> Linnaeus, 1758) (Salmonidae)

Distribution and Heterogeneity of Heterochromatin in the European Huchen (<I>Hucho hucho</I> Linnaeus, 1758) (Salmonidae)

Cytogenetic characterization through chromosomic banding of Pinirampus pirinampu (Pisces, Pimelodidae) from the Tibagi river basin PR/Brazil

NOR and heterochromatin analysis in two cartilaginous fishes by C-, Ag- and RE (restriction endonuclease)-banding

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