Chromosomal Integration of the Extended-Spectrum β-Lactamase Gene bla _CTX-M-15 in Salmonella enterica Serotype Concord Isolates from Internationally Adopted Children

Abstract

We report the emergence of Salmonella enterica isolates of serotype Concord (and its monophasic variant 6,7:l,v:-) producing the extended-spectrum ␤-lactamases (

Long-Term Dissemination of CTX-M-5-Producing Hypermutable Salmonella enterica Serovar Typhimurium Sequence Type 328 Strains in Russia, Belarus, and Kazakhstan

“…Eight isolates of the ST328 clonal group were bla CTX-M-5 positive but pCTXM5 negative by PCR targeting of the conserved segment of the plasmid backbone. The absence of pCTXM5 plasmids in these isolates can be explained by ISEcp1-mediated transposition of the bla CTX-M gene to the chromosome, described in several previous studies (56)(57)(58)(59).…”

Contribution of IncFII and Broad-Host IncA/C and IncN Plasmids to the Local Expansion and Diversification of Phylogroup B2 Escherichia coli ST131 Clones Carrying bla CTX-M-15 and qnrS1 Genes

“…Interestingly, two different ST131 isolates harbored two copies of bla CTX-M-15 , located either on the IncFII (80 kb; rep pC15-1a ) and IncN (55 kb; rep pKP96 ) or on the IncFII (80 kb; rep pC15-1a ) and IncA/C (150 kb; rep p2039 ) plasmids (Table 1). Our results and the detection of different fragments of this MRR in a diversity of plasmid groups from E. coli (IncFII, IncI), K. pneumoniae (IncR, IncFIIk), or Salmonella (IncA/C 2 , IncHI2) and on chromosomes of different species reflect the recombinogenic potential of this region, which can be further spread by widespread clones (4,6,10,14,19).…”

“…or Enterobacter spp. in developing countries) and different conjugative plasmids highlights the risk of further spread of this gene to other successful genetic backgrounds (4,10,14,17,19,21). Differences in pulsed-field gel electrophoresis (PFGE) and virulence profiles among widespread fluoroquinolone-resistant B2 ST131 clones carrying bla CTX-M-15 from different locations indicate frequent local processes of diversification of this lineage, with some of these variants being widely disseminated (5, 13).…”

Salmonella enterica Serotype Gambia with CTX-M-3 and armA Resistance Markers: Nosocomial Infections with a Fatal Outcome

“…A type (e.g., type XGAM-1) was assigned to each PFGE pattern differing by one or more additional bands Ͼ50 kb in size. PCR assays to detect bla TEM , bla CTX-M , and bla SHV were performed on isolates 05-5446 and 05-5447, as described previously (7,8). PCR products were subjected to direct sequencing with an Applied Biosystems sequencer (model ABI 377), and nucleotide sequences were analyzed with the BLAST and ClustalW programs.…”

“…The primers used for PCR detection of the genes encoding 16S rRNA methyltransferase armA were metF and metR, as described by Galimand et al (9). A resistance transfer experiment was carried out by using isolate 05-5447 grown on liquid and solid media, mated with Escherichia coli C1a resistant to nalidixic acid as the recipient strain (8). Transconjugants were selected on Drigalski agar (Bio-Rad, France) supplemented with cefotaxime (4 mg/liter) and nalidixic acid (64 mg/liter).…”