Chromosomal Localization of the Human Gene Encoding c-myc Promoter-Binding Protein (MPB1) to Chromosome 1p35–pter

White, Robert A.; Adkison, Linda R.; Dowler, Lisa L.; Ray, Ratna B.

doi:10.1006/geno.1996.4499

Cited by 16 publications

(15 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, genomic DNAs from mapping panel 2 were digested with HindIII. Southern blots were probed with a human 0.75-kb MIP-2A cDNA as previously described (31). Hybrids were scored for the appropriate human-specific restriction endonuclease fragment on autoradiographs.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A Novel 16-Kilodalton Cellular Protein Physically Interacts with and Antagonizes the Functional Activity of c-myc Promoter-Binding Protein 1

Ghosh¹,

Majumder²,

Steele³

et al. 2001

Molecular and Cellular Biology

Self Cite

View full text Add to dashboard Cite

We initially identified c-myc promoter-binding protein 1 (MBP-1) from a human cervical carcinoma (HeLa) cell expression library (25). MBP-1 is ubiquitously expressed in different human tissues (26) and is located at human chromosome 1p35-pter (31). This protein binds to the TATA box sequences of the c-myc P2 promoter. In vitro transient-transfection assay suggested that MBP-1 negatively regulates both human and mouse c-myc promoter activity (24, 25) through the N-terminal half (27,30). Further studies have shown that MBP-1 and TATA-binding protein (TBP) bind simultaneously in the minor groove of the c-myc P2 promoter (4). Ectopic expression of MBP-1 induces cell death and the reduction of c-myc expression (24) and regresses tumor growth (28). However, Bcl2, a cell survival gene (1, 29), protects against MBP-1-mediated apoptotic cell death, suggesting that, besides c-myc regulation, MBP-1 exerts a regulatory role on cell growth through other unknown mechanisms (24).A recent study indicated that MBP-1, when brought to the promoter by a Gal4 DNA-binding domain, can significantly repress transcriptional activity (7). Structure-function analysis of MBP-1 mutants in the context of the Gal4 DNA-binding domain revealed that MBP-1 transcriptional repressor domains are located in the N terminus (amino acids 1 to 47) and C terminus (amino acids 232 to 338). This further suggests that MBP-1 can modulate cellular gene transcription through an alternative mechanism, besides blocking the c-myc transcription. To understand the regulatory role of MBP-1 in cell growth, it is important to characterize the interacting cellular protein(s) and to evaluate molecular mechanisms involved in MBP-1-mediated transcriptional repression. We undertook a search for cellular proteins that interact with MBP-1 using a two-hybrid interaction cloning strategy in yeast. An MBP-1 interacting protein (MIP-2A) was identified which relieves the transcriptional repression of MBP-1 and antagonizes MBP-1-mediated cell death. Thus, MBP-1 may regulate transcriptional modulation by forming a complex with MIP-2A and also exerts its effect for cell growth regulation. MATERIALS AND METHODSYeast two-hybrid screening. The coding region of MBP-1 was cloned in frame with the LexA DNA-binding domain into the pLexA plasmid vector (Clontech) at BamHI and XhoI restriction sites. The yeast strain EGY48 carrying both the Leu and LacZ reporter genes was transformed with Lex-MBP-1 plasmid DNA and selected positive clones on a synthetic dropout (sd) His Ϫ Ura Ϫ agar plate. The positive yeast colonies were tested for MBP-1 expression by Western blot analysis using anti-LexA monoclonal antibody (Clontech). Lex-MBP-1 positive yeast cells were grown in appropriate liquid medium lacking histidine and uracil and transformed with human cervical carcinoma (HeLa) cell cDNA library (kindly provided by Alain Nepveu, McGill University, Montreal, Quebec, Canada) constructed in pB42AD plasmid vector under the control of an inducible

show abstract

Section: Methodsmentioning

confidence: 99%

“…MBP-1 is ubiquitously expressed in different human tissues (26) and is located at human chromosome 1p35-pter (31). This protein binds to the TATA box sequences of the c-myc P2 promoter.…”

mentioning

confidence: 99%

A Novel 16-Kilodalton Cellular Protein Physically Interacts with and Antagonizes the Functional Activity of c-myc Promoter-Binding Protein 1

Ghosh¹,

Majumder²,

Steele³

et al. 2001

Molecular and Cellular Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…MBP-1, ubiquitously expressed in different human tissues (9), is located at human chromosome 1p35-pter (10). MBP-1, an ϳ37-kDa cellular protein, has many intriguing properties such as transcriptional repressor activity, induction of apoptosis, and regression of tumor growth (8,(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

Ghosh

Steele

Ray

2005

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Prostate cancer is the most common and invasive type of cancer among American men, and the second leading cause of cancer-elated deaths in the United States. Unfortunately, an effective therapeutic regimen is still lacking for advance stages of the disease. Recently, MEK5 has been shown to overexpress in prostate cancer and is associated with poor survival outcome. MEK5 exists as ␣-and ␤-isoforms.

show abstract

“…MBP-1 is expressed ubiquitously in normal human tissues (27) and localized at human chromosome 1p35-ter (38). Ectopic expression of MBP-1 in murine fibroblasts (NIH 3T3 cells) induces massive cell death, DNA fragmentation, and reduction of c-myc expression (26).…”

mentioning

confidence: 99%

Functional Domains of c-myc Promoter Binding Protein 1 Involved in Transcriptional Repression and Cell Growth Regulation

Ghosh¹,

Steele²,

Ray

1999

Molecular and Cellular Biology

Self Cite

View full text Add to dashboard Cite

c-myc promoter binding protein 1 (MBP-1), initially identified from a human cervical carcinoma (HeLa) cell expression library, binds to the TATA box sequences of the c-myc P2 promoter and negatively regulates both human and mouse c-myc promoter activities (6,25,26). The c-myc proto-oncogene can promote cell proliferation, differentiation, and oncogenic transformation (7,36) or apoptosis under certain conditions (9, 35). Regulation of c-myc occurs at multiple levels, such as the initiation or termination of transcription and the attenuation of transcription (20,25). Recent studies have shown that MBP-1 and TATA binding protein bind simultaneously in the minor groove of the c-myc P2 promoter (6). It is possible that MBP-1 negatively regulates c-myc expression by preventing formation of a transcription initiation complex with a general transcriptional factor(s).MBP-1 is expressed ubiquitously in normal human tissues (27) and localized at human chromosome 1p35-ter (38). Ectopic expression of MBP-1 in murine fibroblasts (NIH 3T3 cells) induces massive cell death, DNA fragmentation, and reduction of c-myc expression (26). Bcl2, a cell survival gene, protects against MBP-1-mediated cell death. Complementation of exogenous deregulated c-myc (without an MBP-1 binding site) also prevents MBP-1-induced cell death. Since MBP-1 negatively regulates c-myc transcription, downregulation of endogenous c-myc expression, which is compensated for by exogenous deregulated c-myc, may be a possible mechanism of protection from apoptotic cell death (26). However, the protective role of Bcl2 in MBP-1-mediated cell death suggests the involvement of another cell regulatory factor(s) in the mediation of biological activity of MBP-1. Thus, in addition to c-myc regulation, MBP-1 appears to exert a regulatory effect on cell growth through another, unknown, mechanism. Exogenous expression of MBP-1 in human breast carcinoma cells results in reduced invasiveness, loss of anchorage-independent growth, and suppression of tumor formation in athymic nude mice (28). Recent studies suggest that the C-terminal half of MBP-1 does not bind to the c-myc promoter (29). However, the Cterminal half of MBP-1 suppressed c-myc transcription and reduced cell growth. The mechanism by which MBP-1 exerts its biological activity is unknown. However, one reasonable explanation is that MBP-1 directly or indirectly modulates the expression of other genes necessary for cell proliferation. In this study, we embarked on a detailed analysis of MBP-1-related functional activities. We have used a number of MBP-1 deletion mutant proteins fused to the DNA binding domain of GAL4 to map its transcriptional regulatory activity. The repressor domains identified in the context of the GAL4 system correlated with the biological activities of MBP-1. MATERIALS AND METHODSCells. NIH Swiss mouse embryo (NIH 3T3) cells, human cervical carcinoma (HeLa) cells, and African monkey kidney (COS7) cells were obtained from the American Type Culture Collection. Cells were grown in Dulbecco's modif...

show abstract

Chromosomal Localization of the Human Gene Encoding c-myc Promoter-Binding Protein (MPB1) to Chromosome 1p35–pter

Cited by 16 publications

References 3 publications

A Novel 16-Kilodalton Cellular Protein Physically Interacts with and Antagonizes the Functional Activity of c-myc Promoter-Binding Protein 1

A Novel 16-Kilodalton Cellular Protein Physically Interacts with and Antagonizes the Functional Activity of c-myc Promoter-Binding Protein 1

c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

Functional Domains of c-myc Promoter Binding Protein 1 Involved in Transcriptional Repression and Cell Growth Regulation

Contact Info

Product

Resources

About