Chromosomal localization of the telomeric (TTAGGG)n sequence in eight species of New World Primates (Neotropical Primates, Platyrrhini)

Abstract

Chromosomal localization of the telomeric sequence (TTAGGG)_n in eight New World Primates (Platyrrhini) (Alouatta caraya, Alouatta palliata, Alouatta guariba clamitans, Aotus azarae, Ateles chamek, Cebus nigritus, Cebus paraguayanus, and Saimiri boliviensis) using Fluorescence In Situ Hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe and their possible relationship with the C-banding pattern were analyzed. FISH showed telomeric signals only at the terminal regions of chromosomes from all the species analyzed. Although all of them showed centromeric C+ bands and different size and location of extracentromeric C+ bands, none, except Aotus azarae exhibited (peri)centromeric interstitial telomere-like sequences (ITS). The presence of ITS in Aotus azarae was limited to one pair of submetacentric chromosomes and very likely represents telomeric sequences remaining after a fusion event of ancestral chromosomes during karyotype evolution. Therefore, our data indicate that the distribution of heterochromatin blocks do not correlate with the presence of ITS. However, we cannot rule out the possibility that simple ITS arrays with a few copies of the (TTAGGG)_n sequence, not detectable by conventional FISH, might play a role in the karyotypic evolution of Ceboidea. Further FISH and molecular studies will be needed to confirm this hypothesis.

Chromosomal distribution of interstitial telomeric sequences in nine neotropical primates (Platyrrhini): possible implications in evolution and phylogeny

“…This work extends a previous analysis in New World monkeys (Mudry et al. ). The choice of these species was based on the fact they are products of a rapid radiation and are characterized by high genome plasticity and reshuffled karyotypes (Dumas et al.…”

“…As occurred, for example, in two other Cebus species previously analysed, Cebus nigritus and Cebus paraguayanus (Mudry et al. ).…”

“…) , Cebus nigritus, Cebus paraguayanus, Aotus azarae and Saimiri boliviensis boliviensis (Cebidae) (Mudry et al. ). We extended these analyses, identifying ITSs localizations in other New World monkeys by hybridizing the PNA probe on Callithrix argentata, Callithrix jacchus, Cebuella pygmaea, Saguinus oedipus, Saimiri sciureus, Aotus lemurinus griseimembra, Aotus nancymaae, Lagothrix lagotricha and Callicebus moloch .…”

“…, , ; Mudry et al. ; Bolzán ); (2) to the mechanism of genome reorganization such as double DNA strand break repair, as demonstrated through a comparative molecular approach, analysing DNA sequences in Chinese hamster and in many primates (Faravelli et al. ; Nergadze et al.…”

An annotated checklist of the mammals of Paraguay

“…The taxonomy of Neotropical primates has changed considerably in the past few years, reflecting current research efforts focusing on the group across the South American continent. Although Paraguayan specimens have been used in regional studies including morphology (Arístide et al 2014), genetics (Matayoshi et al 1986;Mudry et al 1987;Pargament and Slavutsky 1987;Martínez et al 2004;Mudry et al 2007;Casado et al 2010), phylogenetics and phylogeography (Hoyos et al 2016), ecology (Boyle 2014) and zoonosis (Díaz et al 2007), little research has been done on this group within Paraguay; the few works available focus on rare or uncommon species such as Mico melanurus (Stallings and Mittermeier 1983), Aotus azarae , and on the more common Alouatta caraya (Giordano and Ballard 2010). Conservation status of primates in Paraguay was assessed by Stallings (1985) and Rumiz and Stallings (1989) and has not been revised since.…”