Chromosomal response of insect and mammalian cells to streptonigrin: A comparative study

Bolzán, Alejandro D.; Bianchi, Néstor O.; Bianchi, Martha S.

doi:10.1002/(sici)1098-2280(1998)32:4<331::aid-em6>3.0.co;2-0

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…During the log phase of growth, the cells were treated for 20 min with SN (Sigma, CAS number 3930-19-6) at the concentrations indicated below. The concentrations employed were based on previous studies carried out in our laboratory [Bolzán et al, 1998[Bolzán et al, , 2001b. At the end of the pulse treatment with the drug, the cells were washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium containing 10 g/ml BrdUrd (Sigma, CAS number 59-14-3) until harvesting.…”

Section: Cell Culture Drug Treatments and Cell Harvestingmentioning

confidence: 99%

Analysis of streptonigrin‐induced incomplete chromosome elements and interstitial fragments in Chinese hamster cells using a telomeric PNA probe

Bolzán

Bianchi

2004

Environ and Mol Mutagen

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We investigated the induction of incomplete chromosome elements (ICEs; i.e., elements with a telomeric signal at only one terminal end) and interstitial fragments induced by the antibiotic streptonigrin (SN) in a Chinese hamster embryo (CHE) cell line using FISH with a telomeric peptide nucleic acid probe. CHE cells were treated with 0-250 ng/ml SN and chromosomal aberrations were analyzed in the first mitosis after treatment using the telomeric probe. Exposure of CHE cells to SN resulted in a linear concentration-related increase in all of the aberration types analyzed (P < 0.05) except ring chromosomes. Depending on the SN concentration employed, 33-68% of the metaphases contained one or more pairs of ICEs (an incomplete chromosome accompanied by a terminal fragment or two incomplete chromosomes accompanied by a compound fragment). Pooled data from all SN concentrations revealed that 77.8% of the acentric fragments were terminal fragments, 18.8% interstitial fragments, and 3.4% compound fragments. Furthermore, it was estimated that about 80% of excess acentric fragments induced by SN originated from incomplete exchanges or terminal deletions and 20% from complete exchanges (interstitial deletions). These results show that incomplete chromosomes and terminal fragments are the most frequent asymmetrical chromosomal aberrations induced by SN and indicate that true incompleteness is a very common event following exposure to SN.

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Section: Cell Culture Drug Treatments and Cell Harvestingmentioning

confidence: 99%

Analysis of streptonigrin‐induced incomplete chromosome elements and interstitial fragments in Chinese hamster cells using a telomeric PNA probe

Bolzán

Bianchi

2004

Environ and Mol Mutagen

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“…On the other hand, although the clastogenic effects of SN are well documented [DuFrain et al, 1980;DuFrain, 1983;Wasserman et al, 1990;Linscombe et al, 1991;Testoni et al, 1993Testoni et al, , 1995Testoni et al, , 1996Bolzán et al, 1998a], the mechanism by which this compound induces chromosome damage has not yet been fully elucidated. In a first attempt to determine the mechanism underlying the clastogenic action of SN, we recently investigated the effect of several antioxidant compounds on the yield of chromosomal aberrations (CAs) induced by this antibiotic on CHO cells [Testoni et al, 1997].…”

Section: Introductionmentioning

confidence: 99%

Modulation of streptonigrin's clastogenic effects in CHO cells by the metal‐chelating agent 1,10‐phenanthroline

Bolzán

Bianchi

Correa

2001

Environ and Mol Mutagen

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The effect of the metal-chelating agent 1,10-phenanthroline (PNT) on the clastogenesis induced by streptonigrin (SN) in CHO cells was investigated. When CHO cells were exposed to SN, chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) were formed in a dose-dependent manner (P < 0.05). When PNT was present in the culture medium, the production of CAs by SN was strongly inhibited (inhibition range = 54.9-80.8%). Similarly, the induction of SCEs by SN was significantly decreased by the addition of PNT to CHO cultures (P < 0.05), although the effect was minor. This finding suggests that intracellular transition metals are implicated in the clastogenesis by SN, and that the Fenton reaction (Fe(2+) + H2O2 --> OH* + OH(-) + Fe(3+)) may be responsible for the production of CAs by this compound. Moreover, the fact that PNT did not completely inhibit the induction of SCEs by SN suggests that this phenomenon might be attributable to a different mechanism, in which transition metals and free radicals play a minor role.

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Structure and function of “metalloantibiotics”

Ming

2003

Medicinal Research Reviews

205

127

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Although most antibiotics do not need metal ions for their biological activities, there are a number of antibiotics that require metal ions to function properly, such as bleomycin (BLM), streptonigrin (SN), and bacitracin. The coordinated metal ions in these antibiotics play an important role in maintaining proper structure and/or function of these antibiotics. Removal of the metal ions from these antibiotics can cause changes in structure and/or function of these antibiotics. Similar to the case of "metalloproteins," these antibiotics are dubbed "metalloantibiotics" which are the title subjects of this review. Metalloantibiotics can interact with several different kinds of biomolecules, including DNA, RNA, proteins, receptors, and lipids, rendering their unique and specific bioactivities. In addition to the microbial-originated metalloantibiotics, many metalloantibiotic derivatives and metal complexes of synthetic ligands also show antibacterial, antiviral, and anti-neoplastic activities which are also briefly discussed to provide a broad sense of the term "metalloantibiotics."

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Chromosomal response of insect and mammalian cells to streptonigrin: A comparative study

Cited by 8 publications

References 28 publications

Analysis of streptonigrin‐induced incomplete chromosome elements and interstitial fragments in Chinese hamster cells using a telomeric PNA probe

Analysis of streptonigrin‐induced incomplete chromosome elements and interstitial fragments in Chinese hamster cells using a telomeric PNA probe

Modulation of streptonigrin's clastogenic effects in CHO cells by the metal‐chelating agent 1,10‐phenanthroline

Structure and function of “metalloantibiotics”

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