Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster

Bender, Welcome; Spierer, Pierre; Hogness, David S.

doi:10.1016/s0022-2836(83)80320-9

Cited by 601 publications

(191 citation statements)

References 22 publications

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“…DNA was extracted from individual flies using previously described methods (Bender et al, 1983). A ∼540 bp fragment of the cytochrome COI gene was PCR amplified as a single product using previously published universal primers: CI-J-1632 (+): 5 -TGATCAAATTTATAAT-3 and CI-N-2191 (−): 5 -GGTAAAATTAAAATATAAACTTC-3 and amplification conditions originally described by Simon et al (1994) and later modified by Kambhampati and Smith (1995).…”

Section: Molecular and Phylogenetic Characterizationmentioning

confidence: 99%

Phylogeography of the neotropical sand fly Lutzomyia longipalpis inferred from mitochondrial DNA sequences

Arrivillaga

Norris

Feliciangeli

et al. 2002

Infection, Genetics and Evolution

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Section: Molecular and Phylogenetic Characterizationmentioning

confidence: 99%

Phylogeography of the neotropical sand fly Lutzomyia longipalpis inferred from mitochondrial DNA sequences

Arrivillaga

Norris

Feliciangeli

et al. 2002

Infection, Genetics and Evolution

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“…ДНК экстрагировали из сердечной мышечной ткани, фиксированной в 70%-ном этаноле, методом щелочного лизиса (Bender et al, 1983). Амплификацию проводили в 25 мкл реакционной смеси, со-держащей ПЦР буфер (0.01 M трис-НCl, 0.05 M KCl, 0.1% тритон Х-100), 4 мМ MgCl 2 , 0.2 мМ каждого из dNTPs, 1 мкл раствора тотальной ДНК, 2.5 мМ прайме-ра и 0.2 ед/мкл Tag-полимеразы («Fermentas») в следующем режиме: 94ºС -7 мин, затем 40 циклов: 94ºС -30 с, 52(56)ºС -45 с, 72ºС -2 мин; 72ºС -7 мин.…”

Section: материал и методыunclassified

Genetic diversity in populations of the silver crucian carp Carassius auratus gibelio (Cyprinidae, Cypriniformes) as depends on reproduction type and reservoir size.

Урюпина¹,

Zhigileva²,

Kultysheva³

et al. 2016

PJE

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Поступила в редакцию 24.07.15 г.Генетическое разнообразие популяций серебряного карася Carassius auratus gibelio (Cyprinidae, Cypriniformes) в зависимости от типа размножения и размера водоёма. -Жигилева О. Н., Култышева М. Е., Сватов А. Ю., Урюпина М. В. -Представлены дан-ные о полиморфизме межмикросателлитных последовательностей ДНК серебряного карася Carassius auratus gibelio в водоёмах юга Западной Сибири. Доля полиморфных ISSR-PCR-бэндов в популяциях карася из разных озёр варьировала в пределах от 40 до 70%, показа-тель генного разнообразия Нея -от 0.16 до 0.25. В популяциях, представленных преимуще-ственно самками, показатели генетического разнообразия были меньше по сравнению с обоеполыми популяциями. Наибольшие показатели полиморфизма выявлены в популяциях, состоящих из диплоидно-триплоидных комплексов. Показатель генного разнообразия попу-ляции карася положительно коррелирует с размером водоёма (r = +0.90, p = 0.015; Rs = 0.74, p = 0.036).Ключевые слова: серебряный карась, Carassius auratus gibelio, полиморфизм, ISSR, гене-тическая структура популяций, Западная Сибирь.Genetic diversity in populations of the silver crucian carp Carassius auratus gibelio (Cyprinidae, Cypriniformes) as depends on reproduction type and reservoir size. -Zhigileva O. N., Kultysheva M. E., Svatov A. Yu., and Urupina M. V. -The paper presents data on the polymorphism of the interior simple sequence repeats of DNA in the silver crucian carp Carassius auratus gibelio from waterbodies in the Western Siberia. The proportion of the polymorphic ISSR-PCR bands in the silver crucian carp populations from different lakes varied from 40 to 70%, the rate of Nei's gene diversity being 0.16 -0.25. Genetic diversity indicators were lower in the populations primarily presented by females, as compared with bisexual ones. The highest levels of genetic polymorphism were revealed in the populations of diploid-triploid complexes. The gene diversity level of the silver crucian carp population positively correlates with the reservoir's size (r = = +0.90, p = 0.015; Rs = 0.74, p = 0.036).

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“…In the early 80's strategies were developed for cloning developmental control genes. The first genes cloned were the hox genes Bithorax-by Hogness and his colleagues (Bender et al, 1983) ( and Antennapedia-by the Gehring group (Carrasco et al, 1984). This was possible because these transcription factor genes contain a large highly conserved region-the homeobox-which encodes a 60 amino acid DNA binding domain and can be picked up by homology screening.…”

Section: Fig 1 Hox Gene Phenotypes the Function Of Hox Genes Is Indmentioning

confidence: 99%

Hox Genes: Master Regulators of the Animal Bodyplan

Durston¹

2012

Embryogenesis

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Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster

Cited by 601 publications

References 22 publications

Phylogeography of the neotropical sand fly Lutzomyia longipalpis inferred from mitochondrial DNA sequences

Phylogeography of the neotropical sand fly Lutzomyia longipalpis inferred from mitochondrial DNA sequences

Genetic diversity in populations of the silver crucian carp Carassius auratus gibelio (Cyprinidae, Cypriniformes) as depends on reproduction type and reservoir size.

Hox Genes: Master Regulators of the Animal Bodyplan

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