Chromosome aberration assays in barley (Hordeum vulgare) a report of the U.S. environmental protection agency Gene-Tox program

Constantin, M.J.; Nilan, R.A.

doi:10.1016/0165-1110(82)90028-8

Cited by 42 publications

(5 citation statements)

References 27 publications

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“…Mutagenic activity of chemicals has been analysed with different plant systems such as Allium cepa (Fiskesjo, 1997), Vicia faba (Koppen and Verschaeve, 1996), Trifolium repens (Citterio et al, 2002), and Tradescantia virginiana (Fomin et al, 1999). Chromosome aberration assays, mutation assays, cytogenetic tests and specific locus mutation assays were performed (Constantin and Nilan, 1982;Tardiff et al, 1994;Marcon et al, 1999) with these systems. The advantage of measuring the effect of genotoxic chemical directly on DNA is mainly related to the sensitivity and the short response time.…”

Section: Introductionmentioning

confidence: 99%

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Labra

Fabio²,

Grassi

et al. 2003

Chemosphere

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In recent years several plant species have been in use as bioindicators and several tests have been developed to evaluate the toxicity of environmental pollutants in vegetal organisms. In the present paper Arabidopsis thaliana (L.) Heynh. (ecotype Wassilewskija) was used as bioindicators of two genotoxic substances: potassium dichromate and dihydrophenanthrene. Inhibition of seed germination was observed with both pollutants. AFLP analysis (i) indicated that both substances are genotoxic, (ii) showed that dihydrophenanthrene induces DNA changes in different target sequences than potassium dichromate, (iii) quantified the genotoxic effect using cluster analysis by comparing DNA from treated plants with that of control plants. On the basis of these considerations we suggest that AFLP method is a powerful tool for measuring qualitative and quantitative genotoxic activity due to environmental pollutants. AFLP method can be applied to a wide range of bioindicator organisms and may become a universal methodology to identify target genes for specific genotoxic agents. This could open up possibilities for designing specifically targeted assays and new approaches to risk assessment.

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Section: Introductionmentioning

confidence: 99%

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Labra

Fabio²,

Grassi

et al. 2003

Chemosphere

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“…A large database has been accumulated on chemicals tested in the barley assay as this species was used extensively for the study of ionizing radiation-induced mutations and chromosome aberrations (Nilan 1974). A detailed protocol for the barley assay is given by Constantin & Nilan (1982). The plant is normally self-fertilized so that recessive mutations are phenotypically expressed.…”

Section: Tradescantia As a Sentinel For Airborne Environmental Mutagensmentioning

confidence: 99%

Higher Plant Assays for the Detection of Genotoxicity in Air Polluted Environments

Grant

1998

Ecosystem Health

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Since the beginning of time pollution has been created by human activities. However, the intensity and the severity of the different kinds of pollution have emerged only in the last few decades and many intricate facets have been revealed. It is gradually being recognized that our health and the ecosystem are being severely affected by environmental chemicals that enter the atmosphere. Plants comprise a large portion of our biosphere and constitute a vital link in the food chain. In this review, types of mutagenic chemicals found both indoor and outdoor are given and several higher plant bioassays for screening and monitoring environmental mutagens are discussed. One plant ideal for monitoring and testing for air pollution is Tradescantia. This species has been used to test for mutagenicity of radioisotope‐contaminated air following the Chernobyl nuclear accident and monitoring around nuclear power plants. One of the greatest contributions of plant bioassays will be their continued use for ambient air monitoring and testing for genotoxicity which can be carried out on a global scale.

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“…Advantages for their use have been cited in a number of studies (Ehrenberg 1971, Kihlman 1971, 1975b, Grant et al 1981, Constantin and Nilan 1982, Constantin and Owens 1982, Grant andZura 1982, Ma 1982, Uggla and Natarajan 1982, Ma and Harris 1985). Kihlman and Sturelid 1978, Andersson et al 1981, Andersson 1981, 1985.…”

Section: Studiesmentioning

confidence: 99%

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster

Grant

1989

CYTOLOGIA

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Chromosome aberration assays in barley (Hordeum vulgare) a report of the U.S. environmental protection agency Gene-Tox program

Cited by 42 publications

References 27 publications

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Higher Plant Assays for the Detection of Genotoxicity in Air Polluted Environments

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

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