Chromosome analysis of mouse one-cell androgenones derived from a sperm nucleus exposed to topoisomerase II inhibitors at pre- and post-fertilization stages

Tateno, Hiroyuki; Kamiguchi, Yujiroh

doi:10.1016/j.mrfmmm.2004.07.007

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…Previous research has reported that spermatozoal chromosomal damage occurs in some extreme environments (Tateno et al 2000, Tateno & Kamiguchi 2004. Furthermore, in our study, fewer embryos that had been injected with NaOH-treated spermatozoa reached the M/B stage than did those injected with fresh spermatozoa.…”

Section: Influence Of Naoh Treatment Of Spermatozoa Dnasupporting

confidence: 38%

Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI

Li¹,

Mizutani²,

Ono³

et al. 2009

Reproduction

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In mammals, ICSI is now a very important tool for both assisted reproductive technology and studying the mechanisms of fertilization. In the latter experiments, it is important to use spermatozoa that have lost their oocyte activation capacity but still retain their developmental potential. In this study, we used high-concentration NaOH to remove oocyte activation potential from spermatozoa, and examined whether normal offspring could be generated from these spermatozoa after ICSI. The spermatozoa were treated with different concentrations of NaOH (1-100 mM) for 1 h and then neutralized with equal amounts of same concentration of HCl. In 10 mM NaOHtreated spermatozoa, the cell membrane was broken and most of them failed to activate oocytes after their injection into the oocytes. However, these spermatozoa did not show strong damage, and after artificial activation with SrCl 2 , all of the zygotes were judged as normal by immunostaining to check the methylation status of histone H3 lysine 9, low chromosome damage by karyotype assay and staining with DNA double-strand breaks marker, gH2AX. Moreover, after transferring those embryos into recipient females, 106 (36.7%) live and healthy offspring were delivered, which is similar to the rate in the fresh control group. By contrast, spermatozoa treated with lower NaOH concentrations retained their oocyte activation capacity and those treated with higher concentrations lost their developmental potential. This suggests that 10 mM NaOH for 1 h is the best treatment to completely destroy the cell membrane and activation capacity of spermatozoa without injuring their developmental potential.

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Section: Influence Of Naoh Treatment Of Spermatozoa Dnasupporting

confidence: 38%

Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI

Li¹,

Mizutani²,

Ono³

et al. 2009

Reproduction

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“…In addition, mouse acrosome enzymes can potentially induce deformation and degeneration of oocytes [30], and the removal of sperm plasma membrane and acrosome before ICSI improves embryonic development in mice [31]. Because inhibition of topological rearrangement of DNA during sperm chromatin remodeling causes DNA lesions [32,33], it is highly possible that the alteration of sperm chromatin remodeling by the acrosomal plasma membrane cholesterol and/or the acrosome can lead to structural chromosome aberrations of paternal origin. TYH is widely used for mouse IVF program because it can effectively promote sperm capacitation and trigger the acrosome reaction [34,35].…”

Section: Discussionmentioning

confidence: 99%

Chromosome aberrations in mouse embryos and fetuses produced by assisted reproductive technology

Tateno

2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The occurrence of structural chromosome aberrations in mouse one-cell embryos produced by intracytoplasmic sperm injection (ICSI) with mature spermatozoa was dependent on the type of sperm incubation medium and sperm incubation time. When cauda epididymal spermatozoa were used following incubation in bicarbonate-buffered TYH medium for 0 h (no incubation) and 0.5 h, the chromosome aberration rates (6.9% and 7.4%, respectively) in the resultant embryos were significantly higher than that (2.3%) in the IVF embryos. However, when the spermatozoa were incubated for 2−2.5 h and 6 h in the same medium, the chromosome aberration rates were reduced to the IVF embryo level (3.8% and 4.3%, respectively). When spermatozoa incubated in Hepes-buffered H-mCZB and phosphate-buffered PB1 media were used for ICSI, chromosome aberration rates in embryos were significantly high (8.6−28.1%) and increased in a time-dependent manner. On the other hand, when immature testicular spermatozoa were incubated in those three media for 0.5 h and 6 h, the incidences of resultant embryos with structural chromosome aberrations ranged between 7.4% and 11.7%, and there was no medium-and time-dependent change in these aberration rates.To evaluate transmissible risk of chromosome aberrations to offspring, two-or four-cell embryos derived from cauda epididymal spermatozoa were transferred into the oviducts of pseudopregnant females and chromosomes of live fetuses were examined on gestational day 16. One (2.0%) mosaic fetus was found when spermatozoa were incubated in TYH for 2−2.5 h, and there were four (6.7%) fetuses displaying a structurally abnormal karyotype when spermatozoa were incubated in H-mCZB for 2−2.5 h, indicating that structural chromosome aberrations generated in ICSI one-cell embryos are transmissible to offspring. The causal mechanism of structural chromosome aberrations in ICSI one-cell embryos is discussed in relation to the acrosomal plasma membrane cholesterol and the acrosome.

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“…Several reports suggest a role for Top2 after fertilization during sperm decondensation and pronuclear formation. However, it is not clear whether this activity in the oocyte is due to paternally or maternally derived enzyme (Bizzaro et al 2000, St Pierre et al 2002, Tateno & Kamiguchi 2004). Regardless, inheritance of an intact sperm nuclear matrix, regulated by Top2, is expected to be essential to the initial stages of development as it likely orders the paternal chromatin structure.…”

Section: The Sperm Nuclear Matrixmentioning

confidence: 99%

The sperm nucleus: chromatin, RNA, and the nuclear matrix

Johnson¹,

Lalancette²,

Linnemann³

et al. 2011

Reproduction

169

110

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Within the sperm nucleus the paternal genome remains functionally inert and protected following protamination. This is marked by a structural morphogenesis that is heralded by a striking reduction in nuclear volume. Despite these changes, both human and mouse spermatozoa maintain low levels of nucleosomes that appear non-randomly distributed throughout the genome. These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that may poise downstream epigenetic effects. This notion is supported by increasing evidence that the embryo inherits these differing levels of chromatin organization. In concert with the suite of RNAs retained in the mature sperm they may synergistically interact to direct early embryonic gene expression. Irrespective, these features reflect the transcriptional history of spermatogenic differentiation. As such they may soon be utilized as clinical markers of male fertility. In this review we explore and discuss how this may be orchestrated.

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Chromosome analysis of mouse one-cell androgenones derived from a sperm nucleus exposed to topoisomerase II inhibitors at pre- and post-fertilization stages

Abstract: Mouse spermatozoa and androgenetic one-cell embryos (androgenones) at various developmental stages were exposed to etoposide (1 µM), a topoisomerase II (topo II) poison, or to either of two catalytic inhibitors:

Cited by 13 publications

References 40 publications

Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI

Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI

Chromosome aberrations in mouse embryos and fetuses produced by assisted reproductive technology

The sperm nucleus: chromatin, RNA, and the nuclear matrix

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