Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (−)-epigallocatechin gallate (EGCG)

Kusakabe, Hirokazu; Kamiguchi, Yujiroh

doi:10.1016/j.mrgentox.2004.08.005

Cited by 11 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is probable that mouse spermatozoa incompletely undergo in vitro capacitation in these media. The previous results also indicate that the increased incidence of structural chromosome aberrations is associated with the uncapacitated state of spermatozoa; there is a trend toward a higher incidence of aberrations (6%-9%) in ICSI embryos derived from spermatozoa after short incubation (5-15 min) in H-mCZB medium [4][5][6]13] compared with ICSI embryos (4%) derived from spermatozoa after a long incubation (;90 min) in TYH medium [16].…”

mentioning

confidence: 79%

“…Intracytoplasmic sperm injection (ICSI) into mouse metaphase II (MII) oocytes has enabled us to observe chromosomes of immotile (dead) mouse [1][2][3][4][5][6][7], whale [8], and human [9][10][11] spermatozoa, as well as morphologically abnormal mouse [12,13] and human spermatozoa [14,15], although the conventional in vitro fertilization (IVF) technique is not traditionally used for such purposes. These studies have yielded useful information on the chromosome damage of such defective spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse

Tateno

Kamiguchi

2007

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

To investigate whether cytogenetic risks occur using the mouse intracytoplasmic sperm injection (ICSI) technique, the incidence of chromosome aberrations was compared in one-cell embryos produced by ICSI technique and those by conventional in vitro fertilization (IVF) technique. Spermatozoa were incubated in TYH medium for 1.5-2 h before IVF insemination. For the ICSI technique, spermatozoa were incubated in five different media: TYH, Hepes-buffered TYH (H-TYH), modified CZB (mCZB), Hepes-buffered mCZB (H-mCZB), and PB1 for 0.5 h, 2-2.5 h, and 6 h before injection into metaphase II oocytes. The incidence of IVF embryos with structural chromosome aberrations was 2%, whereas the occurrence of structural chromosome aberrations in ICSI embryos was dependent on the kind of medium and sperm incubation time. When spermatozoa were incubated in TYH medium for 2 h or more, the aberration rates in the resultant ICSI embryos (4%) were not significantly different from that of IVF embryos. However, there was a significant increase in aberration rates in ICSI embryos derived from spermatozoa that were incubated in other culture conditions (6%-28%). In addition, a time-dependent increase in aberration rates was found in ICSI embryos when H-TYH, H-mCZB, and PB1 were used for sperm incubation. There was no significant difference in incidence of aneuploidy between IVF and ICSI embryos. The chromosome analysis results of one-cell embryos were reflected by the performance of postimplantation embryo development. The causal mechanism of chromosome damage in ICSI embryos was discussed in relation to the plasma membrane cholesterol, the acrosome, and in vitro aging of spermatozoa.

show abstract

mentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse

Tateno

Kamiguchi

2007

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…EGCG reportedly has two different actions: an antioxidant action at lower concentrations and a pro‐oxidant action at higher concentrations (Chen et al. 2003; Kusakabe and Kamiguchi 2004). EGCG can protect cell membranes from lipid peroxidation (Chen et al.…”

Section: Discussionmentioning

confidence: 99%

“…EGCG can protect cell membranes from lipid peroxidation (Chen et al. 2003), whereas it acts as a pro‐oxidant and damages the DNA and membranes of sperm (Kusakabe and Kamiguchi 2004). Therefore, a high concentration of EGCG supplementation may change the plasma membrane integrity and may cause a decrease in sperm viability.…”

Section: Discussionmentioning

confidence: 99%

“…Bars with different letters within each boar differ significantly (p < 0.05) different actions: an antioxidant action at lower concentrations and a pro-oxidant action at higher concentrations (Chen et al 2003;Kusakabe and Kamiguchi 2004). EGCG can protect cell membranes from lipid peroxidation (Chen et al 2003), whereas it acts as a pro-oxidant and damages the DNA and membranes of sperm (Kusakabe and Kamiguchi 2004). Therefore, a high concentration of EGCG supplementation may change the plasma membrane integrity and may cause a decrease in sperm viability.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of (−)‐Epigallocatechin Gallate on the Motility and Penetrability of Frozen–Thawed Boar Spermatozoa Incubated in the Fertilization Medium

Kaedei

Naito

Naoi

et al. 2012

Reprod Domestic Animals

View full text Add to dashboard Cite

Epigallocatechin gallate (EGCG) is the major polyphenol in green tea (Camellia sinensis) and is known for its antioxidant effects. The objective of the present study was to examine the effects of EGCG during in vitro fertilization (IVF) on the sperm quality and penetrability into oocytes. In the first experiment, the effects of concentration and incubation period of EGCG on the motility and penetrability of spermatozoa were examined. When frozen-thawed spermatozoa were incubated in IVF medium supplemented with 0 (control), 1, 50 and 100 μm EGCG for 1, 3 and 5 h, supplementation with 50 and 100 μm EGCG improved motility of the spermatozoa (p < 0.05), but not viability, as compared with the control group. When frozen-thawed spermatozoa were co-incubated with in vitro-matured (IVM) oocytes in IVF medium supplemented with 50 and 100 μm EGCG for 5 h, supplementation of EGCG had positive effects on sperm penetration rates. In the second experiment, the effects of supplementation of EGCG in IVF medium on penetrability of sperm from different boars and development of fertilized oocytes were evaluated. When frozen-thawed spermatozoa from six boars were co-incubated with IVM oocytes in IVF medium supplemented with 50 μm EGCG, the effect of EGCG on sperm penetration and development of oocytes after fertilization was found to vary with individual boar. Our results indicate that motility and penetrability of boar spermatozoa are improved by co-incubation with 50 μm EGCG, but the effects vary with individual boars.

show abstract

Spermiogenesis in Sperm Genetic Integrity

2011

View full text Add to dashboard Cite

Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (−)-epigallocatechin gallate (EGCG)

Cited by 11 publications

References 24 publications

Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse

Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse

Effects of (−)‐Epigallocatechin Gallate on the Motility and Penetrability of Frozen–Thawed Boar Spermatozoa Incubated in the Fertilization Medium

Spermiogenesis in Sperm Genetic Integrity

Contact Info

Product

Resources

About