Chromosome analysis of some Phaseolus vulgaris L. genotypes in Turkey

Kulaz, Haluk; Najafi, Solmaz; Tunçtürk, Murat; Tunçtürk, Rüveyde; Yılmaz, Hilal

doi:10.3329/bjb.v51i2.60425

Cited by 2 publications

(3 citation statements)

References 8 publications

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“…The chromosome number in all genotypes was 2n = 2x = 22, confirming several previous results [17,[19][20][21][22][23][24][27][28][29][30]. The MTCL (Mean Total Chromosome Length) recorded in the studied genotypes varied from 15.65 ± 0.99 to 34.24 ± 0.35 micrometers (Table 2), which is almost similar to the previous study on nine genotypes of the common bean, who's MTCL was measured to be between 13.74 and 29.50 micrometers [24].…”

Section: Discussionsupporting

confidence: 89%

“…Many classical and molecular cytogenetic studies have been performed on this plant, but since these characteristics are variable under the influence of geographical and ecological factors [15,16], it seems necessary to study the characteristics of the nuclear and chromosomal genomes separately in each region. Several cytogenetic studies have been conducted to investigate the chromosomal structure of the common bean, which include chromosome analysis and cytogenetic maps [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Genome size information is an important issue in ploidy analysis, genome analysis, taxonomy, evolution, and breeding studies [31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Kulaz

Najafi

Tunçtürk

et al. 2022

Genes

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The common bean (Phaseolus vulgaris L.), whose annual production is 26 million tons worldwide, is one of the main sources of protein and is known as one of the most important food sources. In this study, the karyotype variations and the genome size of four common bean genotypes in Turkey were investigated to determine whether the geographic variables in these regions affected the genome size and the karyotype parameters. In addition, it is known that as that the cytological and chromosomal parameters change under the influence of the climatic conditions of each region, appropriate and stable cytological methods for each plant facilitate and enable the determination of the chromosomal structure and the identification of specific chromosomes in the genotypes of the relevant region. Correct and valuable information such as this enables breeders and researchers to determine the correct shape and actual size of chromosomes. The genome size of the genotypes was measured with a flow cytometer, and chromosome analyses were performed with the squash method. For each genotype, the karyotype parameters, such as the number of somatic chromosomes, the Mean Total Chromosome Length (MTCL), the Mean Centromere Index (MCI), and the Mean Arm Ratio (MAR), were measured. The results showed that the highest and the lowest amounts of DNA per nucleus (3.28 pg and 1.49 pg) were observed in the Bitlis and Elaziğ genotypes. In addition, all genotype chromosome numbers were counted to be 2n = 2x = 22. The Mean Total Chromosome Length varied from 15.65 µm in Elaziğ to 34.24 µm in the Bitlis genotype. The Mean Chromosome Length ranged between 1.42 µm and 3.11 µm in the Elaziğ and Bitlis genotypes. The Hakkari and Van genotypes consist of eleven metacentric chromosomes, while the Bitlis and Elaziğ genotypes consist of ten metacentric chromosomes and one sub-metacentric chromosome. However, the Mean Centromere Index and Arm Ratio differed considerably among the genotypes. The highest (46.88) and the lowest (43.18) values of the Mean Centromere Index were observed in the Hakkari and Elaziğ genotypes, respectively. On the other hand, the lowest (1.15) and the highest (1.36) values of the Mean Arm Ratio were obtained in the Bitlis and Elaziğ genotypes, respectively. Eventually, intraspecies variations in genome size and chromosomal parameters were observed, and it was determined that the changes in nuclear DNA content and different chromosomal parameters among the four Phaseolus genotypes from four different regions of Turkey indicate the effect of climate change in the regions on these parameters. Such information in these areas can be used as useful information for the improvement of this plant and breeding programs.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Kulaz

Najafi

Tunçtürk

et al. 2022

Genes

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“…Phaseolus vulgaris L. (the common bean) is in the practical and economical crop group used to solve nutritional problems and eliminate protein deficits in the daily diet [ 1 ]. The common bean, one of the most widely grown edible legumes in the world, contains high amounts of protein (18–24%), carbohydrates (56%), and vitamins A, B, and C [ 2 , 3 , 4 , 5 ]. It is also rich in iron (Fe) and zinc (Zn) elements, which are defined as trace elements necessary for human health [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of MTP (Metal Ion Transport Proteins) Genes in the Common Bean

Yilmaz,

Özer,

Baloch

et al. 2023

Plants

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MTP/CDF carriers, called metal ion transport proteins, act as substrates for the transmission of micronutrients such as iron (Fe), zinc (Zn), and manganese (Mn) to membrane carriers in plants. In this study, genome-wide analysis of the MTP gene family in the common bean genome, expression analysis of the PvMTP4, PvMTP5, and PvMTP12 genes after Fe and Zn treatments, and the effects of Fe and Zn applications on iron and zinc content were investigated. This study used common bean genotypes assumed to have high or low Fe and Zn accumulation ability. PvMTP genes were defined as containing conserved catalytic domains with molecular weights and protein lengths ranging from 41.35 to 91.05 kDa and from 369 to 813 amino acids (aa), respectively. As a result of the phylogenetic analysis, three main clusters containing seven subgroups were formed. In this study, the first characterization of the MTP gene family of beans was performed, and the responses of three different PvMTP genes in the Zn-CDF group to Fe and Zn applications were revealed. The obtained findings are thought to constitute pioneering resources for future research on common bean biofortification studies, plant breeding related to Fe and Zn, and the functional characterization of the MTP gene family.

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Chromosome analysis of some Phaseolus vulgaris L. genotypes in Turkey

Cited by 2 publications

References 8 publications

Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Genome-Wide Identification and Expression Analysis of MTP (Metal Ion Transport Proteins) Genes in the Common Bean

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