Chromosome doubling in Paspalum notatum var. saure (cultivar Pensacola)

Weiler, Roberto Luis; Krycki, Karine Cristina; Guerra, Divanilde; Simioni, Carine; Dall'Agnol, Miguel

doi:10.1590/1984-70332015v15n2n19

Cited by 15 publications

(15 citation statements)

References 18 publications

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“…The native P. notatum germplasm from Rio Grande do Sul is tetraploid and displays apomictic reproduction (Forbes and Burton, 1961). Completely sexual tetraploid plants were produced directly from sexual diploids plants of natural bahiagrass populations by colchicine treatment (Quarin evaluation of segregating progeny Weiler et al, 2015). The subsequent use of duplicate genotypes in intraspecific mating systems opens new possibilities for breeding programs (Rebozzio et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Weiler

Dall'Agnol

Simioni

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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For many decades, animal production in southern Brazil has been based on native forage grasses. Paspalum notatum Flügge (bahiagrass) is one of the most frequently used native grass in southern Brazil. The native germplasm of P. notatum is tetraploid and displays apomictic reproduction; hence, chromosome doubling of sexual diploid accessions is required to facilitate hybridization. The main goal of this study was to evaluate forage production and other agronomical evaluation of P. notatum intraspecific hybrids in order to obtain new varieties that could be registered and/or protected and launched as new cultivars in the future. Three tetraploid sexual genotypes were crossed with the ecotypes Bagual and André da Rocha. Biomass production (leaf, stem, and inflorescence fractions), plant growth habit, plant height, number of tillers, and frost damage were evaluated in the resulting hybrids. The general combining ability (GCA) and specific combining ability (SCA) were calculated for the six families generated. We evaluated 196 hybrids and the most productive yielded 42 % more total dry mass than the most productive parent (ecotype Bagual), and Bagual production was seven-fold higher than that of cultivar 'Pensacola'. The highest GCA values were observed for Bagual and Q4205. No correlation was observed between leaf coloration and the frost effect or regrowth in the following winter.Similarly, no correlation between plant growth habit and dry mass production was observed. The reproduction mode of the most productive hybrids are currently being analyzed in larger plots to identify apomictic and sexual genotypes for future registration and protection.

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Section: Introductionmentioning

confidence: 99%

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Weiler

Dall'Agnol

Simioni

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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“…The three plants confirmed tetraploidy (2n=4x=40), as described by Weiler et al (2015). Chromosome pairing was typical of tetraploidy, with univalent, bivalent, trivalent and quadrivalent chromosome associations (Table 2).…”

Section: Analysis Of Meiotic Behaviormentioning

confidence: 59%

“…The three plants tetraploidized by Weiler et al (2015) were evaluated, nominated WKS 3, WKS 63, and WKS 92, regarding the cytoembryological, cytogenetic and pollen viability analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, it is possible to protect the developed cultivar. Weiler et al (2015) artificially duplicated chromosomes of three individuals of P. notatum (Pensacola bahiagrass) by immersing flower buds and seeds for different exposure time in various concentrations of colchicine, which is an antimitotic agent responsible for chromosome duplication. The three duplicated plants, WKS 3, WKS 63, and WKS 92, confirmed the tetraploid level of ploidy and the complete euploidy by the root tip analysis and meiotic cells diakinesis.…”

Section: Introductionmentioning

confidence: 99%

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Cytoembryological evaluation, meiotic behavior and pollen viability of Paspalum notatum tetraploidized plants

Krycki

Simioni

Dall'Agnol

2016

Crop Breed. Appl. Biotechnol.

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“…Sexual tetraploid bahiagrass germplasm has been developed by doubling the chromosomes of sexual diploid accessions, including Pensacola bahiagrass (Forbes and Burton, 1961;Quarin et al, 2001;Quesenberry et al, 2010;Weiler et al, 2015). This unique sexual germplasm provides a means of breeding improved forage cultivars at the tetraploid level by crossing the sexual induced tetraploid plants with naturally occurring apomictic tetraploids.…”

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confidence: 99%

Storage of Bahiagrass Pollen at Different Temperatures

et al. 2018

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Paspalum is an important genus of grasses, and some species are used for forage in the warmer regions of the world. Efforts to develop improved cultivars often require intra‐ and interspecific hybridization, but asynchronous flowering sometimes prevents hybridization between parental species. One method of circumventing this problem is to store pollen using cryopreservation so pollen will be available to pollinate the female parent when it flowers. Bahiagrass (Paspalum notatum Flügge) was used to determine if Paspalum pollen can be preserved using long‐term cold storage. Pollen was dehydrated with LiCl, MgCl2, NaOH, and silica gel for 30, 60, and 120 min. Both dehydrated and nondehydrated pollen were stored at 4, −20, and −196°C for 10, 60, 120, and 180 d. After thawing, pollen stainability and in vivo germination were used to assess viability. Stainability of fresh pollen was 73.3%, and for treated and stored pollen, it ranged from 25.3 to 71.3%. Storage at −20 and −196°C was superior to storage at 4°C. Pollen dehydrated with LiCl for 30 min stored best at −196°C; pollen dehydrated for 120 min with silica gel and for 30 and 60 min with MgCl2 stored best at −20°C. All samples regardless of treatment, storage temperature, or length of storage had viable pollen. These findings demonstrate that bahiagrass pollen can be stored in long‐term cold conditions, and this procedure can be used to circumvent problems associated with asynchronous flowering between parental species used in wide hybridization programs.

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Chromosome doubling in Paspalum notatum var. saure (cultivar Pensacola)

Abstract: -

Cited by 15 publications

References 18 publications

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Cytoembryological evaluation, meiotic behavior and pollen viability of Paspalum notatum tetraploidized plants

Storage of Bahiagrass Pollen at Different Temperatures

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