Chromosome relationships among 13 species and one variety of Lachenalia (Liliaceae) with the chromosome numbers of 2n=14 and 16 detected by FISH using 5S rDNA and 18S rDNA probes and DAPI staining

Hamatani, Shuichi; Tagashira, Norikazu; Ishida, Genjiro; Kondo, Katsuhiko

doi:10.3199/iscb.4.57

Cited by 4 publications

(7 citation statements)

References 8 publications

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“…This data may represent that two pairs of same chromosomes were exist on the L. congesta genome. However L. muirii which had chromosome number 2n=14 (X=7) also had four signals of 5S rDNA (Hamatani et al 2009), so that four signals of 5S rDNA detected on diploid species might be common feature of genus Lachenalia.…”

Section: Resultsmentioning

confidence: 98%

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Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Hamatani¹,

Masuda

Uchida

et al. 2012

Chromosome Botany

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ABSTRACT. The ploidy level and the phylogenic position of Lachenalia congesta W. F. Barker (Asparagaceae) were analyzed by karyomorphological, molecular cytogenetical and molecular phylogenetical studies. The chromosome number of L. congesta was 2n=28, and these chromosomes showed bi-modal karyotype with four large sized chromosomes and 24 small sized chromosomes. The ploidy level of this species was diploid, because each two chromosomes made 14 pairs on the karyotype analysis by acetoorcein staining, DAPI staining and FISH methods. In addition two signals of 18S rDNA sites were detected by the FISH method supported this assumption. All 28 chromosomes of this species did not show any clear DAPI-positive bands and four signals of 5S rDNA sites were detected near the centromeres of small sized chromosomes. These characters were quite different from the characters observed by karyomorphological and molecular cytogenetical studies on common Lachenalia species with basic chromosome number of x=7 or 8. The data from molecular phylogenetic analysis of internal transcribed spacer (ITS) region supported this hypothesis. KEYWORDS: DAPI, FISH, Karyotype, Lachenalia congesta, Phylogenetic analysis, ITSChromosome Botany (2012) Lachenalia is distributed in the western part of South Africa and the southern part of Namibia, and includes ca. 110 species. This genus was formerly classified in the Hyacinthaceae or Liliaceae, although now it revised in the Asparagaceae followed to the third Angiosperm Phylogeny Group (APG III) classification (The Angiosperm Phylogeny Group 2009).Lachenalia congesta W. F. Barker is a dwarf species and is found on mountain slopes of Eastern Cape interior at up to alt. 2400 m with large colonies (Duncan 2009). Duncan (2009) described that Lachenalia congesta was a very distinctive species which cannot be easily mistaken against any other member of Lachenalia during the flowering season. It was recognized by two prostrate, broadly lanceolate (or occasionally ovate), leathery leaves that are dark olive-green on the upper surface and deep magenta below, with prominent dark maroon margins (Duncan 2009). Johnson and Brandham (1997) reported that the chromosome number of L. congesta was 2n=26, 28, although any other karyomorphological information about this species were not described. The molecular phylogenetic analysis using chlorophyll (cp) DNA data of genus Lachenalia indicated that L. congesta was closely related to six Polyxena species (these were transferred to genus Lachenalia by Manning et al. 2004) and three Lachenalia species (Spies 2004), although phylogenetic information from nuclear DNA analysis were not reported.In this report, we attempt to identify the ploidy level of L. congesta by the karyomorphological analysis and fluorescence in situ hybridization (FISH) methods, and to clarify the molecular phylogenetic relationship of this species among genus Lachenalia on the basis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. MATERIALS AND METHODSPlant materials Some pla...

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Section: Resultsmentioning

confidence: 98%

“…For molecular cytogenetic study, fluorescent in situ hybridization (FISH), fixation, hydrolysis and squash of root tips followed Hamatani et al (2009). 5S and 18S rDNA probes were used for the labeling.…”

Section: Karyomorphological and Molecular Cytogenetical Studiesmentioning

confidence: 99%

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Hamatani¹,

Masuda

Uchida

et al. 2012

Chromosome Botany

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“…Root-tips of the plants listed in Table 1 were harvested. The methods of pre-treatment, fi xation, hydrolysis and squash of roottips followed Hamatani et al (2009).…”

Section: Methodsmentioning

confidence: 99%

“…They were amplifi ed according to Sogin (1990) and Hizume (1993), respectively. For FISH experiments, each rDNA probes were labeled by Hamatani et al (2009). FISH method also followed Hamatani et al (2009).…”

Section: Methodsmentioning

confidence: 99%

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Molecular cytogenetic analysis in seven species of Lachenalia (Liliaceae) with the chromosome numbers of 2n=18, 22, 23, 26 and 28 by DAPI staining and FISH using 5S rDNA and 18S rDNA probes

Hamatani¹,

Tagashira

Kondo

2010

Chromosome Botany

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Molecular cytogenetic analysis in seven species of Lachenalia (Liliaceae) with 2n=18, 22, 23, 26 and 28 was studied using DAPI staining and fl uorescence in situ hybridization (FISH). Lachenalia latimerae (2n=18), L. juncifolia (2n=22) and L. zeyheri (2n=23) would be considered as diploids which had basic chromosome numbers of x=9, 11 and 11, respectively. They showed similarities in the characters of DAPI bands and FISH signals of 5S rDNA and 18S rDNA sites. Lachenalia aloides var. vanzyliae (2n=28) and L. orchioides (2n=28) showed tetraploidy (X=7). Lachenalia paucifolia (2n=26) and L. longituba (2n=28), which belonged to the genus Polyxena by different system, considered as diploids which might have in respect to their basic chromosome numbers of x=13 and 14. Lachenalia paucifolia and L. longituba showed different characters of DAPI bands and FISH signals of 5S rDNA and 18S rDNA and showed different characters from the other species of Lachenalia studied here.

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Critically endangered Lachenalia viridiflora - from seeds to flowering bulbs

Kapczyńska

2023

South African Journal of Botany

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Chromosome relationships among 13 species and one variety of Lachenalia (Liliaceae) with the chromosome numbers of 2n=14 and 16 detected by FISH using 5S rDNA and 18S rDNA probes and DAPI staining

Cited by 4 publications

References 8 publications

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Molecular cytogenetic analysis in seven species of Lachenalia (Liliaceae) with the chromosome numbers of 2n=18, 22, 23, 26 and 28 by DAPI staining and FISH using 5S rDNA and 18S rDNA probes

Critically endangered Lachenalia viridiflora - from seeds to flowering bulbs

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