Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)

Kiihl, Paulo Roberto Peres; Pereira, Andréia Rodrigues Alonso; Godoy, Sara Mataroli de; Stenzel, Neusa Maria Colauto; Risso-Pascotto, Claudicéia

doi:10.1590/s0103-84782011005000076

Cited by 12 publications

(6 citation statements)

References 13 publications

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“…As well as, asynchronism occur because these chromosomes do not reach the telophase phase for regular chromosomal segregation (Soares-Scott et al 2003;Corrêa et al 2005;Damasceno et al 2010;Kiihl et al 2011;Souza and Pereira, 2011). In addition, paracentric translocations and inversions can cause the emergence of chromosomal bridges (Levin 2002;Paiva et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

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Interspeci c hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passi ora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, arti cial Passi ora hybrids were developed without many di culties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Con rmation of interspeci c hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander solution, uorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, pairing in 90% of the cells in diakinesis. There was a signi cant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle bers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspeci c hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs. We also hypothesize that interspeci c hybrid genotypes of Passi ora can be obtained with regular meiosis, which could be viable and fertile.

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Section: Discussionmentioning

confidence: 99%

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

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“…is responsible for the formation of anomalous post-meiotic products such as monads, dyads, triads and polyads resulted to the unreduced gametes (Golubovskaya 1989) or sterile (Bosco et al 1999) pollen grains. According to Kiihl et al (2011), the meiotic phases that precede cytoplasm cleavage might have caused failure in the cytokinesis process resulting into monads, dyads, triads and polyads. There are three main types of abnormal spindles orientation i.e.…”

Section: Discussionmentioning

confidence: 99%

Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae)

Dwivedi

2021

Caryologia

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Unreduced gametes are the key source for the natural polyploidization in plants, but process of its formation is very low in nature. Meiotic mutants are second source for the formation of 2n pollen. In this cytological investigation, the meiotic aberrations and its impact on post-meiotic products were analysed in autotetraploid Trachyspermum ammi (L.) Sprague (4n=36). The seedlings of T. ammi (L.) Sprague were treated with 3 different concentrations of colchicine (0.2, 0.4 and 0.5%, w/v) for 3 different durations. Six polyploid plants were induced which was confirmed on the basis of cytological analysis. Colchicine, an anti-microtubular drug induced different meiotic and post-meiotic abnormalities such as chromosomal bridges, lagging chromosomes, scattering, precocious, fragments, dyads, triads, and polyads. The formation of several abnormal sporads clearly signifies the meiotic restitution. The tendency of univalents to scattered in the cytoplasm at metaphase was identified as a peculiar aberration asynapsis. Pollen variability and fusion of pollen walls was reported and pollen fertility was calculated. The morphological analysis of the pollen allowed us to confirm the occurrence of 2n pollen.

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“…The normal meiotic process produces viable gametes and normal post-meiotic cells, and, consequently, higher plant fertility (Pagliarini 2000). Therefore, meiotic behavior should be studied in detail in plant breeding programs in which inter-and intraspecific hybridizations are required, because it is possible to check for homeologous chromosome pairing, meiotic irregularities, and gamete viability (Kiihl et al 2011). Knowledge of chromosome pairing is important for selecting species that are genetically close for interspecific crosses (Techio and Davide 2007).…”

Section: Discussionmentioning

confidence: 99%

Morphological and cytogenetic characterization of new ornamental Passiflora hybrids (P. ‘Vivis’ and P. ‘Jhovi’)

Souza

Silva

et al. 2020

Crop Breed. Appl. Biotechnol.

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The present study was performed for the morphological and cytogenetic description of F 1 hybrids obtained from a cross between Passiflora coccinea Aubl. and Passiflora hatschbachii Cervi. Hybridization was confirmed by genomic in situ hybridization (GISH). Corona banding was the most relevant floral descriptor for separating the hybrids into two groups, the cultivars P. 'Vivis' and P. 'Jhovi', and the parents and hybrids had diploid chromosome number 2n = 18. The F 1 progenies exhibited normal meiotic behavior with normal tetrad production, which guaranteed high pollen viability (>70%) and fertile hybrids. Therefore, P. 'Vivis' and P. 'Jhovi' have the potential to be used for ornamental plants market.

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Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)

Abstract: ABSTRACT

Cited by 12 publications

References 13 publications

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae)

Morphological and cytogenetic characterization of new ornamental Passiflora hybrids (P. ‘Vivis’ and P. ‘Jhovi’)

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