Chronic Chagas disease: Quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy

Apt, Werner; Carrasco, Daniela; Fuentealba, C; Canals, Mauricio; Muñoz, Gabriela; Saavedra, Miguel; Castillo, J. Guerrero; Zulantay, Inés

doi:10.1016/j.actatropica.2019.105167

Cited by 2 publications

(5 citation statements)

References 49 publications

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“…More complete results were obtained with the qPCR-XD probe, since more positive cases were detected compared to the BS [30], amplifying more T. cruzi DTU TcV, similar to what has been described [21]. The same DTUs could be genotyped in XD samples and BS in cases 8, 9, and 10.…”

Section: Discussionsupporting

confidence: 69%

“…Afterwards, a pool of the dejections analyzed at 30, 60, and 90 days was prepared, diluted in 500 μL PBS pH 7.2 The pool was incubated for 15 min at 98 °C and centrifuged for 3 min at 3500 rpm. The supernatant of 200 µL was stored at −20 °C until its use (while XD is very useful for verifying the presence of viable T. cruzi , we are not currently applying this diagnostic tool) [ 23 , 30 , 65 ].…”

Section: Methodsmentioning

confidence: 99%

“…DNA purification was performed with an initial volume of 200 µL, according to the instructions of the kit QIA amp DNA Blood Mini Kit (Qiagen). The eluted was conserved at −20 °C until qPCR-BS was carried out [ 27 , 28 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…eq/mL. Given the variability in the number of copies of the nuclear satellite DNA previously described, the curve was made with equal quantities (20 ng of each one/200 μL blood) of the clonal reference strains Dm28c (TcI highest number of copies) and Y (TcII lowest number of copies) to reduce the differences in the detection limits [ 23 , 24 , 26 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Afterwards, a pool of the dejections analyzed at 30, 60, and 90 days was prepared, diluted in 500 µL PBS pH 7.2 The pool was incubated for 15 min at 98 • C and centrifuged for 3 min at 3500 rpm. The supernatant of 200 µL was stored at −20 • C until its use (while XD is very useful for verifying the presence of viable T. cruzi, we are not currently applying this diagnostic tool) [23,30,65] Peripheral BS was obtained by venipuncture and was stored in Venoject tubes with guanidine HCl-EDTA in 1:1 volume (2 mL blood in 2 mL of preservant anticoagulant solution). The samples were incubated at 98 • C for 15 min to favor the breaking of T. cruzi minicircles and were conserved at 4 • C until DNA extraction.…”

Section: Biological Samples Of Xenodiagnosismentioning

confidence: 99%

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Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study

et al. 2022

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Chagas disease (ChD) is a vector zoonosis native to the American continent caused by the protozoan parasite Trypanosoma cruzi; the biological vectors are multiple species of hematophagous insects of the family Triatominae. A relevant aspect in the host–parasite relationship is the identification of the various genotypes of T. cruzi called discrete typing units (DTU) that circulate in mammals and vectors. In Chile, it has been described that the DTUs TcI, TcII, TcV, and TcVI circulate in infected humans, vectors, and wild animals. Identifying DTUs has acquired clinical importance, since it has been suggested that different genotypes could cause distinct pathologies, circulate in different geographical areas, and present different sensitivities to trypanocidal drugs. In this study, circulating T. cruzi DTUs in peripheral blood and Triatoma infestans dejections used in xenodiagnosis (XD) were amplified by qPCR in 14 Chilean patients with chronic ChD from highly endemic areas. More positive samples were detected by XD compared to peripheral blood samples, and 64.28% of the cases were simple infections and 35.72% mixed, with a statistically significant difference in the frequency of TcV DTU. This study would suggest that T. infestans from Chile is more competent to amplify one DTU over others, probably due to a process of co-evolution.

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Section: Discussionsupporting

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Biological Samples Of Xenodiagnosismentioning

confidence: 99%

See 3 more Smart Citations

Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study

et al. 2022

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Risk Factors for Progression to Chronic Chagas Cardiomyopathy: A Systematic Review and Meta-Analysis

Cutshaw

Sciaudone

Bowman

2023

The American Journal of Tropical Medicine and Hygiene

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Approximately one-third of people with chronic Trypanosoma cruzi infection develop Chagas cardiomyopathy, which carries a poor prognosis. Accurate prediction of which individuals will go on to develop Chagas cardiomyopathy remains elusive. We performed a systematic review of literature comparing characteristics of individuals with chronic Chagas disease with or without evidence of cardiomyopathy. Studies were not excluded on the basis of language or publication date. Our review yielded a total of 311 relevant publications. We further examined the subset of 170 studies with data regarding individual age, sex, or parasite load. A meta-analysis of 106 eligible studies indicated that male sex was associated with having Chagas cardiomyopathy (Hedge’s g: 1.56, 95% CI: 1.07–2.04), and a meta-analysis of 91 eligible studies indicated that older age was associated with having Chagas cardiomyopathy (Hedge’s g: 0.66, 95% CI: 0.41–0.91). A meta-analysis of four eligible studies did not find an association between parasite load and disease state. This study provides the first systematic review to assess whether age, sex, and parasite load are associated with Chagas cardiomyopathy. Our findings suggest that older and male patients with Chagas disease are more likely to have cardiomyopathy, although we are unable to identify causal relationships due to the high heterogeneity and predominantly retrospective study designs in the current literature. Prospective, multidecade studies are needed to better characterize the clinical course of Chagas disease and identify risk factors for progression to Chagas cardiomyopathy.

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Chronic Chagas disease: Quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy

Cited by 2 publications

References 49 publications

Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study

Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study

Risk Factors for Progression to Chronic Chagas Cardiomyopathy: A Systematic Review and Meta-Analysis

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