“…Immunofluorescent staining: The OSW and MCA samples were permeabilized with 0.025% Triton X-100 (Fisher Scientific, #BP151-100), and blocked with freshly prepared 10% Donkey serum with 1% Bovine Serum Albumin (Gemini Bio-Products, #700-100P) for 2 hours. After blocking, the following primary antibodies were applied, and left overnight at 4˚C: mouse monoclonal anti-human neutrophil elastase (NE) (1:100, Dako, Clone NP57, #M0752); 56,57 rabbit polyclonal to histone H3 (1:100, citrulline R2+R8+R17, 1:100, Abcam, #ab5103); 58,59 rabbit polyclonal Keratin-14 (1:1000, BioLegend, #905301, Covance #PRB-155P); 60,61 rabbit polyclonal anti-human Myeloperoxidase (MPO) (1:100, Abcam, #ab45977); 62,63 rabbit monoclonal anti-human CD3 (1:100, Abcam, #ab16669); 64,65 rabbit polyclonal anti-Oncostatin M (LifeSpan Biosciences, #LS-C104796); rabbit polyclonal NGAL (1:100, Abcam, #ab63929); 66,67 and rabbit polyclonal LIGHT/TNFSF14 (1:100, LifeSpan Biosciences, #LS-C118682). Isotype controls were used as negative controls.…”