“…Wild-type, male, 6–8 week old C57BL/6 mice (Jackson Laboratories, Bar Harbor, ME) were treated with 10mg of nebulized Pseudomonas aeruginosa endotoxin from Sigma-Aldrich (L9143, St Louis, MO) dissolved in phosphate buffered saline (PBS), or PBS alone using an Aeroneb nebulizer (Aerogen, Galway, Ireland) as described previously (Fig 1A). 23 One hour later, mice were exposed to 1.4% isoflurane in 100% oxygen for 2 hours as described previously. 24 Briefly, mice were randomly assigned to receive 1.4% isoflurane in 100% oxygen or 100% oxygen alone with identical flow rates in identical anesthetizing chambers.…”