Chronic ethanol treatment: dolichol and retinol distribution in isolated rat liver cells

Cottalasso, Damiano; Bassi, Anna Maria; Canepa, Corrado; Maloberti, Giuseppe; Casu, A; Nanni, Giorgio

doi:10.1016/s0891-5849(02)01291-1

Cited by 9 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3), as observed previously (Cottalasso et al, 2003), the percentage of the isoprenoid Dol-18 increases by 7-12% in all liver cells isolated from control rats. With this supplementation, Dol-18 decreases after TAA treatment and does not normalise at any time in the liver cells of the surviving animals.…”

Section: Dolichol and Dolichol Isoprenoidssupporting

confidence: 81%

See 1 more Smart Citation

Effect of a load of Vitamin A after acute thioacetamide intoxication on dolichol, dolichol isoprenoids and retinol content in isolated rat liver cells

et al. 2004

Self Cite

View full text Add to dashboard Cite

Section: Dolichol and Dolichol Isoprenoidssupporting

confidence: 81%

“…Control rats received a modified Lieber-De Carli liquid diet without ethanol (Lab. Piccioni, Milano, Italy), as described (Cottalasso et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Effect of a load of Vitamin A after acute thioacetamide intoxication on dolichol, dolichol isoprenoids and retinol content in isolated rat liver cells

et al. 2004

Self Cite

View full text Add to dashboard Cite

“…In previous studies we found a percentage increase in dolichol with 18 isoprene units (Dol 18) in all hepatic cells isolated from rats after vitamin A administration [12]; this indicates that, at least in liver cells, the metabolic pathways of retinol and dolichol might be interrelated. We were therefore prompted to investigate in vivo whether the association of POH with vitamin A, a known antioxidant isoprenoid and modulator of differentiation, might provide a rational basis for future therapy, since no data on this issue are as yet available.…”

Section: Discussionmentioning

confidence: 57%

“…Control rats received a modified Lieber-De Carli liquid diet (without ethanol, Lab. Piccioni, Milano, Italy), as described [12].…”

Section: Animals and Hepatocyte Isolationmentioning

confidence: 99%

“…We had previously found an alteration of dolichol isoprenoid content in liver after in vivo treatment with many toxicants [8][9][10]. Because FPP and GPP are also substrates of cis-prenyltransferases that synthesise dolichol [11], we hypothesised that the availability of dolichol precursors, that is to say isoprenoid intermediates FPP and GGPP or others, might be impaired or deviated into other cellular metabolic pathways [12].Interest in protein prenylation stems from the fact that many proteins require prenylation for their biological and/or oncogenic functions. The use of inhibitors of FTases and GGTases therefore seems to be rational with a view to inhibiting…”

mentioning

confidence: 99%

See 1 more Smart Citation

Protein and m-RNA expression of farnesyl-transferases, RhoA and RhoB in rat liver hepatocytes: action of perillyl alcohol and vitamin A in vivo.

et al. 2005

View full text Add to dashboard Cite

We analysed the action, in rats in vivo, of the protein isoprenylation inhibitor perillyl alcohol (POH) and that of vitamin A, alone or in association, on m-RNA and protein expression of farnesyltransferases (FTases alpha and beta subunits) and their protein substrates RhoA and RhoB, in isolated hepatocytes. Combined administration of POH and vitamin A induced a sharp decrease in FTase alpha protein after 96 h, suggesting an involvement not only of farnesyltransferases but also of geranylgeranyltransferases, which share the FTase alpha protein. FTase beta protein did not decrease. POH plus vitamin A, in contrast with POH or vitamin A alone, induced a decrease in RhoB protein, probably because of different cleavages. No modification was observed in RhoA protein. Vitamin A alone increased RhoB m-RNA and protein expression. As one of the functions of RhoB is cell polarisation, these data support our previous hypothesis of a polarised transport of vitamin A from hepatocytes to hepatic stellate cells. As the behaviours of m-RNAs and proteins in this study were often different, cytoplasmic metabolic pathways must be considered for the parameters studied. The behaviour of Rho B, which is thought to have an antioncogene function, is discussed in view of its isoprenylated forms in the membranes. These preliminary findings stress the need, when studying the association of two isoprenoids in cancer therapy, to consider normal as well as tumour-bearing animals.

show abstract

Morphological and oxidative alterations on Sertoli cells cytoskeleton due to retinol-induced reactive oxygen species

et al. 2005

View full text Add to dashboard Cite

Retinol (vitamin A) is involved in several cellular processes, like cell division, differentiation, transformation and apoptosis. Although it has been shown that retinol is a limitant factor for all these processes, the precise mechanisms by which retinol acts are still unknown. In the present study we hypothesised that alterations in the cytoskeleton of Sertoli cells induced by retinol supplementation could indicate an adaptive maintenance of its functions, since it plays an important role in the transformation process that we observed. Previous results demonstrated that Sertoli cells treated with retinol showed an oxidative imbalance, that leads the cell to two phenotypes: apoptosis or transformation. Our group has identified characteristics of Sertoli cells transformed by retinol which results in normal cell functions modification. In the present study the actin filament fluorescence assay and the deformation coefficient showed a modification in the morphology induced by retinol. We also observed an oxidative alteration in isolated cytoskeleton proteins and did not show alterations when these proteins are analyzed by electrophoreses. Our results showed an increase in mitochondria superoxide production and a decrease in nitric oxide levels. All results were partially or completely reverted by co-treatment of the antioxidant Trolox. These findings suggest that the cytoskeleton components suffer individual alterations in different levels and that these alterations generate a global phenotype modification and that these processes are probably ROS dependent. We believe that the results from this study indicate an adaptation of the cytoskeleton to oxidative imbalance since there was not a loss of its function.

show abstract

Chronic ethanol treatment: dolichol and retinol distribution in isolated rat liver cells

Cited by 9 publications

References 43 publications

Effect of a load of Vitamin A after acute thioacetamide intoxication on dolichol, dolichol isoprenoids and retinol content in isolated rat liver cells

Effect of a load of Vitamin A after acute thioacetamide intoxication on dolichol, dolichol isoprenoids and retinol content in isolated rat liver cells

Protein and m-RNA expression of farnesyl-transferases, RhoA and RhoB in rat liver hepatocytes: action of perillyl alcohol and vitamin A in vivo.

Morphological and oxidative alterations on Sertoli cells cytoskeleton due to retinol-induced reactive oxygen species

Contact Info

Product

Resources

About