Chronic exposure to the parasite Enteromyxum leei (Myxozoa: Myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, Sparus aurata L.

Sitjà‐Bobadilla, Ariadna; Calduch-Giner, Josep Àlvar; Saera-Vila, Alfonso; Palenzuela, Oswaldo; Álvarez-Pellitero, Pilar; Pérez‐Sánchez, Jaume

doi:10.1016/j.fsi.2008.01.014

Cited by 81 publications

(91 citation statements)

References 76 publications

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“…The resulting prevalence of infection was 67.8%, mean intensity of infection was high, and fish were classified intothree groups; CTRL, (non-exposed), R-PAR (recipient fish exposed to the parasite and parasitized), and RNonPAR (recipient fish exposed to theparasite, but not parasitized). For more details see Sitjà-Bobadilla et al (2008). …”

Section: Parasite Diagnosismentioning

confidence: 99%

“…Thus far, the humoral and cellular innate immune responses of gilthead sea bream (Cuesta et al, 2006a(Cuesta et al, , 2006bSitjà-Bobadilla et al, 3 2007; Sitjà- and sharpsnout sea bream (Golomazou et al, 2006;Muñoz et al, 2007;Álvarez-Pellitero et al, 2008) have been studied. Furthermore, the expression level of a small number of immunorelevant genes has been investigated (Cuesta et al, 2006b;Sitjà-Bobadilla et al, 2008) and clear differences have been observed in the expression profile of some of these genes between infected and non-infected fish. However, the exact mechanisms underlying these differences remain to be established.…”

Section: Introductionmentioning

confidence: 99%

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Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Davey¹,

Calduch-Giner²,

Houeix³

et al. 2011

Molecular Immunology

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Section: Parasite Diagnosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Davey¹,

Calduch-Giner²,

Houeix³

et al. 2011

Molecular Immunology

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“…In our model of chronic parasite infection in which samples were taken in a time much longer that all the pathogen models thus far studied, it was noteworthy that the host expression of most PRDX isoforms was significantly down-regulated in head kidney. This fact can be viewed as a disease outcome at the later stages of the infection, in which an anti-inflammatory stage is promoted by the host to avoid the excessive immune reaction, as suggested by the lower IL-1β and TNF-α observed in the intestine of R-PAR fish [40]. In contrast and importantly, the transcription rate of NKEFs and PRDX3 was significantly up-regulated in R-NON PAR fish.…”

Section: Discussionmentioning

confidence: 97%

“…To analyze the effect of three types of challenging experimental conditions on PRDX gene expression, samples were obtained from earlier studies already published [36,39,40]. The infection trial was performed by exposure of juvenile fish (134 g initial body weight) to E. leei-contaminated effluent [40].…”

Section: Methodsmentioning

confidence: 99%

“…The infection trial was performed by exposure of juvenile fish (134 g initial body weight) to E. leei-contaminated effluent [40]. After 113 days of exposure, two categories of recipient fish were obtained: non-parasitized (exposed but not infected, R-NON PAR) and parasitized (exposed and infected, R-PAR) fish, which were compared with control animals (not exposed to the parasite, CTRL).…”

Section: Methodsmentioning

confidence: 99%

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Molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (Sparus aurata): Insights from fish exposed to dietary, pathogen and confinement stressors

Pérez‐Sánchez

Bermejo-Nogales

Calduch-Giner

et al. 2011

Fish & Shellfish Immunology

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ABSTRACT. The aim of this work was to underline the physiological role of the antioxidant peroxiredoxin (PRDX) family in gilthead sea bream (Sparus aurata L.), a perciform fish extensively cultured in the Mediterranean area. First, extensive BLAST searches were done on the gilthead sea bream cDNA database of the AQUAMAX European Project (www.sigenae.org/iats), and six contigs were unequivocally identified as PRDX1-6 after sequence completion by RT-PCR. The phylogenetic analysis evidenced three major clades corresponding to PRDX1-4 (true 2-Cyst PRDX subclass), PRDX5 (atypical 2-Cys PRDX subclass) and PRDX6 (1-Cys PRDX subclass) that reflected the present hierarchy of vertebrates. However, the PRDX2 branch of modern fish including gilthead sea bream was related to the monophyletic PRDX1 node rather than to PRDX2 cluster of mammals and primitive fish, which probably denotes the acquisition of novel functions through vertebrate evolution. Transcriptional studies by means of quantitative real-time PCR evidenced a ubiquitous PRDX gene expression that was tissue-specific for each PRDX isoform. In a second set of transcriptional studies, liver and head kidney were chosen as target tissues in fish challenged with i) the intestinal parasite Enteromyxum leei, ii) a plant oil (VO) diet with deficiencies in essential fatty acids and iii) prolonged exposure to high rearing densities. These studies showed that PRDX genes were highly and mostly constitutively expressed in the liver and were not affected by dietary intervention or high density. In contrast, head kidney was highly sensitive to the different experimental challenges: significantly lower values were found for PRDX5 in the three trials, for PRDX6 in parasitized and high density fish and for PRDX1 in parasitized and VO fish. PRDX2, 3 and 5 were decreased only in VO, high density and parasitized animals, respectively. These findings would highlight the role of PRDXs as integrative and highly predictive biomarkers of health and welfare in fish and gilthead sea bream in particular.

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Evaluation of ubiquitinated proteins by proteomics reveals the role of the ubiquitin proteasome system in the regulation of Grp75 and Grp78 chaperone proteins during intestinal inflammation

et al. 2013

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The ubiquitin proteasome system (UPS) is the major pathway of intracellular protein degradation and may be involved in the pathophysiology of inflammatory bowel diseases or irritable bowel syndrome. UPS specifically degrades proteins tagged with an ubiquitin chain. We aimed to identify polyubiquitinated proteins during inflammatory response in intestinal epithelial HCT-8 cells by a proteomic approach. HCT-8 cells were incubated with interleukin 1β, tumor necrosis factor-α, and interferon-γ for 2 h. Total cellular protein extracts were separated by 2D gel electrophoresis and analyzed by an immunodetection using antiubiquitin antibody. Differential ubiquitinated proteins were then identified by LC-ESI MS/MS. Seven proteins were differentially ubiquitinated between control and inflammatory conditions. Three of them were chaperones: Grp75 and Hsc70 were more ubiquitinated (p < 0.05) and Grp78 was less ubiquitinated (p < 0.05) under inflammatory conditions. The results for Grp75 and Grp78 were then confirmed in HCT-8 cells and in 2-4-6-trinitrobenzen sulfonic acid induced colitis in rats mimicking inflammatory bowel disease by immunoprecipitation. No difference was observed in irritable bowel syndrome like model. In conclusion, we showed that a proteomic approach is suitable to identify ubiquitinated proteins and that UPS-regulated expression of Grp75 and Grp78 may be involved in inflammatory response. Further studies should lead to the identification of ubiquitin ligases responsible for Grp75 and Grp78 ubiquitination.

show abstract

Chronic exposure to the parasite Enteromyxum leei (Myxozoa: Myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, Sparus aurata L.

Cited by 81 publications

References 76 publications

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (Sparus aurata): Insights from fish exposed to dietary, pathogen and confinement stressors

Evaluation of ubiquitinated proteins by proteomics reveals the role of the ubiquitin proteasome system in the regulation of Grp75 and Grp78 chaperone proteins during intestinal inflammation

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