Chronic high fat feeding paradoxically attenuates cerebral capillary dysfunction and neurovascular inflammation in Senescence-Accelerated-Murine-Prone Strain 8 mice

Lam, Virginie; Stephenson, Andrea; Nesbit, Michael; Mamsa, Somayra; Hackett, Mark J.; Takechi, Ryusuke; Mamo, John

doi:10.1080/1028415x.2019.1664533

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…The poor performance from HF rats indicates the damaging effects of high-fat (in the absence of EPA þ DHA and zerumbone) on the hippocampus/cortex region of the brain, which are primarily involved in learning and memory. Our results are in agreement with earlier reports that demonstrate cognitive decline due to long-term consumption of high-fat diet rich in saturated fats (27,28) . Motor coordination involves body movements comprising kinematic (such as spatial direction) and kinetic (force) parameters that result in the execution of an action/skill.…”

Section: Discussionsupporting

confidence: 94%

Zerumbone augments cognitive enhancement potentials of EPA+DHA: insight from a hyperlipidaemic rat model

et al. 2020

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Abstract Hyperlipidemia and cognitive dysfunction (CD) are the two public health concerns. Though hyperlipidemia has been comprehensively studied in respect of cardiovascular diseases, its role on CD needs to be explored. Hence, we evaluated hyperlipidemia as a risk factor for CD, and the efficacy of eicosapentaenoic acid (EPA, 20:5n-3) + docosahexaenoic acid (DHA, 22:6n-3), and zerumbone (Z) in modulating CD under hyperlipidemic conditions. Male Wistar rats (Rattus norvegicus) were fed control, high-fat (HF), high-fat + fish oil (HF+F), high-fat + zerumbone (HF+Z), and high-fat + fish oil + zerumbone (HF+F+Z) containing diet. After 30 days feeding trial, memory parameters [(Morris water maze, elevated plus maze (transfer latency), and T-maze (spontaneous alteration)], and locomotor skills (open field test and rotarod test) were assessed. Hyperlipidemia significantly (p<0.05) reduced memory and motor coordination skills compared to control. However, the administration of EPA+DHA and zerumbone significantly (p<0.05) restored the hyperlipidemia-induced loss of memory and motor coordination skills. Collectively, our data imply that hyperlipidemia causes CD by decreasing the memory and motor coordination skills, and administration of EPA+DHA and zerumbone prevent hyperlipidemia induced CD. The augmented effect of EPA+DHA, together with zerumbone, discloses a promising strategy for lowering the severity of CD in hyperlipidemic conditions.

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Section: Discussionsupporting

confidence: 94%

Zerumbone augments cognitive enhancement potentials of EPA+DHA: insight from a hyperlipidaemic rat model

et al. 2020

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“…Because HFD is intended to induce obesity in most studies, it is difficult to differentiate the effects of fat from the effects of obesity on inflammation. However, HFD has been shown to reduce inflammation in brains of mice with accelerated aging and in livers of mice with complex III deficiency without inducing weight gain [13,37].…”

Section: Hallmark Of Agingmentioning

confidence: 99%

Are fat and sugar just as detrimental in old age?

et al. 2021

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“…At 7 weeks of age, the mice were anaesthetised with 2% gaseous isoflurane and euthanised via cervical dislocation [ 35 ]. Whole-brain specimens were carefully extracted and immediately snap frozen in liquid nitrogen and kept at −80 °C until analysis.…”

Section: Methodsmentioning

confidence: 99%

A highly sensitive LC-MS/MS method for quantitative determination of 7 vitamin D metabolites in mouse brain tissue

et al. 2023

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Despite its critical role in neurodevelopment and brain function, vitamin D (vit-D) homeostasis, metabolism, and kinetics within the central nervous system remain largely undetermined. Thus, it is of critical importance to establish an accurate, highly sensitive, and reproducible method to quantitate vit-D in brain tissue. Here, we present a novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method and for the first time, demonstrate detection of seven major vit-D metabolites in brain tissues of C57BL/6J wild-type mice, namely 1,25(OH)2D3, 3-epi-1,25(OH)2D3, 1,25(OH)2D2, 25(OH)D3, 25(OH)D2, 24,25(OH)2D3, and 24,25(OH)2D2. Chromatographic separation was achieved on a pentaflurophenyl column with 3 mM ammonium formate water/methanol [A] and 3 mM ammonium formate methanol/isopropanol [B] mobile phase components. Detection was by positive ion electrospray tandem mass spectrometry with the EVOQ elite triple quadrupole mass spectrometer with an Advance ultra-high-performance liquid chromatograph and online extraction system. Calibration standards of each metabolite prepared in brain matrices were used to validate the detection range, precision, accuracy, and recovery. Isotopically labelled analogues, 1,25(OH)2D3-d3, 25(OH)D3-c5, and 24,25(OH)2D3-d6, served as the internal standards for the closest molecular-related metabolite in all measurements. Standards between 1 fg/mL and 10 ng/mL were injected with a resulting linear range between 0.001 and 1 ng, with an LLOD and LLOQ of 1 pg/mL and 12.5 pg/mL, respectively. The intra-/inter-day precision and accuracy for measuring brain vit-D metabolites ranged between 0.12–11.53% and 0.28–9.11%, respectively. Recovery in acetonitrile ranged between 99.09 and 106.92% for all metabolites. Collectively, the sensitivity and efficiency of our method supersedes previously reported protocols used to measure vit-D and to our knowledge, the first protocol to reveal the abundance of 25(OH)D2, 1,25(OH)D2, and 24,25(OH)2D2, in brain tissue of any species. This technique may be important in supporting the future advancement of pre-clinical research into the function of vit-D in neurophysiological and neuropsychiatric disorders, and neurodegeneration. Graphical Abstract

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Chronic high fat feeding paradoxically attenuates cerebral capillary dysfunction and neurovascular inflammation in Senescence-Accelerated-Murine-Prone Strain 8 mice

Cited by 5 publications

References 49 publications

Zerumbone augments cognitive enhancement potentials of EPA+DHA: insight from a hyperlipidaemic rat model

Zerumbone augments cognitive enhancement potentials of EPA+DHA: insight from a hyperlipidaemic rat model

Are fat and sugar just as detrimental in old age?

A highly sensitive LC-MS/MS method for quantitative determination of 7 vitamin D metabolites in mouse brain tissue

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