Chronic intermittent ethanol exposure selectively alters the expression of Gα subunit isoforms and RGS subtypes in rat prefrontal cortex

Luessen, Deborah J.; Sun, Haiguo; McGinnis, Molly M.; McCool, Brian A.

doi:10.1016/j.brainres.2017.07.014

Cited by 7 publications

(3 citation statements)

References 66 publications

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“…GPCRs are negatively regulated by a class of GTPases termed regulator of G-protein signaling (RGS) [ 242 ]. RGS5, a potent negative regulator of Gα q and Gα i , structurally consists of a conserved RGS domain that binds to the corresponding Gα subunit and dephosphorylates the active GTP-bound Gα subunit via the GTPase-stimulating activity of the RGS domain [ 243 , 244 ].…”

Section: Gpcrs-mediated Autophagy In Ischemic Strokementioning

confidence: 99%

The dual roles of autophagy and the GPCRs-mediating autophagy signaling pathway after cerebral ischemic stroke

et al. 2022

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Ischemic stroke, caused by a lack of blood supply in brain tissues, is the third leading cause of human death and disability worldwide, and usually results in sensory and motor dysfunction, cognitive impairment, and in severe cases, even death. Autophagy is a highly conserved lysosome-dependent process in which eukaryotic cells removal misfolded proteins and damaged organelles in cytoplasm, which is critical for energy metabolism, organelle renewal, and maintenance of intracellular homeostasis. Increasing evidence suggests that autophagy plays important roles in pathophysiological mechanisms under ischemic conditions. However, there are still controversies about whether autophagy plays a neuroprotective or damaging role after ischemia. G-protein-coupled receptors (GPCRs), one of the largest protein receptor superfamilies in mammals, play crucial roles in various physiological and pathological processes. Statistics show that GPCRs are the targets of about one-fifth of drugs known in the world, predicting potential values as targets for drug research. Studies have demonstrated that nutritional deprivation can directly or indirectly activate GPCRs, mediating a series of downstream biological processes, including autophagy. It can be concluded that there are interactions between autophagy and GPCRs signaling pathway, which provides research evidence for regulating GPCRs-mediated autophagy. This review aims to systematically discuss the underlying mechanism and dual roles of autophagy in cerebral ischemia, and describe the GPCRs-mediated autophagy, hoping to probe promising therapeutic targets for ischemic stroke through in-depth exploration of the GPCRs-mediated autophagy signaling pathway.

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Section: Gpcrs-mediated Autophagy In Ischemic Strokementioning

confidence: 99%

The dual roles of autophagy and the GPCRs-mediating autophagy signaling pathway after cerebral ischemic stroke

et al. 2022

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“…The groups were administered study agents for 18 days. 12 At the end of the experiment, all animals were anesthetized with ketamine hydrochloride (50 mg/kg; Ketalar ® , Pfizer, Turkey) and xylazine hydrochloride (15mg/ kg; Rompun ® , Bayer, Turkey) administered intraperitoneally. The animals were fixed by transcardial perfusion under anesthesia with heparinized saline solution followed by 10% neutral-buffered formalin.…”

Section: Application Of Experimentsmentioning

confidence: 99%

Stereological examination of effects of ethanol on optic nerve in experimental alcohol model

Igit

Çölçimen

2019

Hum Exp Toxicol

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The objective of the present study was to perform histological and stereological examination of alcohol-induced changes in the optic nerve, considered an extension of white matter of the brain, in rats. This study included 20 male Wistar albino rats aged 60 days and weighing 190–220 g. The rats were divided into three groups: ethanol ( n = 7), maltodextrin ( n = 7), and control ( n = 6) groups. The ethanol group was administered ethanol at a dose of 6.4% (v/v) instead of water for 18 days; the maltodextrin group received maltodextrin for the same time period, and the control group was the sham group. At the end of the experiment, a 0.5-mm long section of the optic nerve starting from the optic chiasma was dissected and examined with routine microscopic histological examination methods. The modified Cavalieri method was used for stereological measurement. Total tissue area ratios were calculated with a point grid provided by the Shtereom 1.5 software package. The statistical comparison of the groups revealed that the ethanol group had a significant reduction in the number of axons and sheath area of the optic nerve compared to the control and maltodextrin groups ( p < 0.017, p < 0.022, respectively). These results indicate the toxic effects of ethanol on the optic nerve.

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“…Recently, Lin group reported that the prediction model of ovarian cancer constructed using RGS family genes was likely to be important to the clinical decision making and the personalized treatment of patients with ovarian cancer [12]. Beyond that, many researches focus mostly on the expression and pathogenesis of speci c members of RGS gene family including RGS2, RGS4, RGS5, RGS12, RGS17 et al [13][14][15]. However, the study on RGS gene family in KIRC is none.…”

Section: Introductionmentioning

confidence: 99%

Pan-cancer multi-omics analysis reveals the prognostic value of RGS gene family

Wu,

Lin,

Zhang

et al. 2024

Preprint

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Background: The regulator of G-protein signaling (RGS) family, regulating cellular signaling events downstream of G-protein coupled receptors (GPCRs), is of great significance for diagnostic and prognostic prediction in cancer. At present, the comprehensive studies of RGS family genes in pan-cancer and specifically in Kidney renal clear cell carcinoma (KIRC) are rare. Methods: The performance of RGS genes in pan-cancer was assessed using the multi-omics dataset including genomic, transcriptomic, epigenetic and clinical data obtained from The Cancer Genome Atlas (TCGA). Subsequently, we conducted an in-depth exploration of RGS family genes in KIRC. Univariate cox regression and lasso regression were used to construct the risk model based on the five RGS genes. Independent prognostic factors for OS of KIRC patients were validated via univariate and multivariate COX analyses, and a nomogram was then developed. Finally, tumor mutation burden, immune infiltration, drug sensitivity and functional enrichment were analyzed and compared between the low- and high-risk groups. Result: We comprehensively found out that the abnormal expression, somatic mutations and methylation of RGS genes were associated with tumorigenesis and survival rates in pan-cancer. Interestingly, much more highly expressed RGS genes induced significantly higher risk and poorer survival in KIRC than those in other tumors. A prediction model for the prognosis based on five RGS genes (RGS2, RGS17, RGS10, RGS20 and RGS7BP) was established using univariable cox regression and lasso regression. The functional enrichment, tumor microenvironment, and immune infiltration were statistically different between the low-risk and high-risk groups. Clinically, our risk score model was effective in predicting the sensitivity of KIRC patients to chemotherapy and immune checkpoint blockade therapy. Conclusions: A five-gene risk-score signature was constructed and validated, which is of great clinical value and contributes to better clinical decision making and personalized treatment strategies associated with the benefits of KIRC patients.

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Chronic intermittent ethanol exposure selectively alters the expression of Gα subunit isoforms and RGS subtypes in rat prefrontal cortex

Cited by 7 publications

References 66 publications

The dual roles of autophagy and the GPCRs-mediating autophagy signaling pathway after cerebral ischemic stroke

The dual roles of autophagy and the GPCRs-mediating autophagy signaling pathway after cerebral ischemic stroke

Stereological examination of effects of ethanol on optic nerve in experimental alcohol model

Pan-cancer multi-omics analysis reveals the prognostic value of RGS gene family

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