Chronic oral exposure to glycated whey proteins increases survival of aged male NOD mice with autoimmune prostatitis by regulating the gut microbiome and anti-inflammatory responses

Chen, Yingjia; Guo, Kevin M.; Nagy, Tamás; Guo, Tai L.

doi:10.1039/c9fo01740b

Cited by 18 publications

(14 citation statements)

References 51 publications

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“…Besides the main results from nal conditional Logistic regression analyses, we also found signs of gut microbial dysbiosis indicated by signi cantly differentiated relative abundances in other taxa. For instance, SCFA producing Anaerostipes was under-represented in GDM group and has been previously found nominally associated with type 2 diabetes mellitus [26], which was elevated along with alleviation of in ammation in animal models [27,28]. On the other hand, we found several taxa including Ruminococcaceae spp., Veillonellaceae and opportunistic pathogens Enterobacteriaceae of Proteobacteria were signi cantly over-represented in GDM cases.…”

Section: Discussionsupporting

confidence: 57%

Association Of Gut Microbiota During Early Pregnancy With Risk Of Incident Gestational Diabetes Mellitus: A Nested Case-Control Study

Chen

Chu

et al. 2020

Preprint

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Background: We aimed to assess gut bacterial biomarkers during early pregnancy and subsequent risk of gestational diabetes mellitus (GDM) in Chinese pregnant women.Methods: Based on the Tongji-Shuangliu Birth Cohort study, we conducted a nested case-control study among 201 incident GDM cases and 201 matched controls individual matched on age, gestational week, and date of fecal sample collection. Fecal samples were collected during early pregnancy, and GDM was diagnosed at 24-28 weeks of pregnancy. Community DNA isolated from fecal samples and V3-V4 region of 16S rRNA gene amplicon libraries were sequenced. Results: In GDM cases versus controls, Rothia, Actinomyces, Bifidobacterium, Adlercreutzia, and Coriobacteriaceae, and Lachnospiraceae spp. were significantly reduced, while Enterobacteriaceae, Ruminococcaceae spp. and Veillonellaceae were over-represented. It was also found that the abundance of Staphylococcus relative to Clostridium, Roseburia and Coriobacteriaceae as reference microorganisms were positively correlated with fasting blood glucose, 1-h postprandial glucose, and 2-h postprandial glucose levels. Conditional Logistic regression showed that 4 microbial taxa during early pregnancy were associated with subsequent GDM risk, including Actinobacteria, Coriobacteriaceae, genus 26 of Coriobacteriaceae and an unknown specie of the Coprococcus.Conclusion: Gut microbiota during early pregnancy was associated with subsequent risk of GDM. Several beneficial and commensal gut microorganisms showed inverse relations with incident GDM, while opportunistic pathogenic members involving in GDM development, where they positively correlated with clinical measurements on OGTT. Our study provided promising biological markers that may be used in monitoring pregnant women’s health and developing therapeutic interventions on GDM at early stage of gestation.

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Section: Discussionsupporting

confidence: 57%

Association Of Gut Microbiota During Early Pregnancy With Risk Of Incident Gestational Diabetes Mellitus: A Nested Case-Control Study

Chen

Chu

et al. 2020

Preprint

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“…Cronin et al found that WP supplementation experienced a significant alteration in the β-diversity of the intestinal flora [ 14 ]. Chen et al reported that WP-derived early EGPs could increase the survival rate of aged male NOD mice, and decrease metabolic syndrome in immune infiltration [ 15 ]. These pieces of evidence indicated that WP could maintain body health and repair damage in many ways.…”

Section: Introductionmentioning

confidence: 99%

Effects of different types and doses of whey protein on the physiological and intestinal flora in D-galactose induced aging mice

Zhang²,

et al. 2021

PLoS ONE

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The elderly usually suffer from many diseases. Improving the quality of life of the elderly is an urgent social issue. In this present study, D-galactose treated aging mice models were used to reveal the effects of different animal sources and different doses of whey protein (WP) on the immune indexes organs and intestinal flora. A total of 9 groups were set up, including normal control (NC), negative control (NS), positive control (Vc), low-, medium- and high-doses of cow WP intervention groups (CL, CM and CH for short, correspondingly) and low-, medium- and high-doses of goat WP intervention groups (GL, GM and GH for short, correspondingly). The body weight gain, thymus/body weight ratio, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, spleen immunoglobulins G (IgG), spleen interleukin-2 (IL-2) and spleen interleukin-2 (IL-6) were measured. Then, the intestinal contents were collected, and 16s genes of intestinal bacteria were sequenced to reveal the changes in bacterial flora structure. WP intervention significantly increased the weight gain, thymus/body ratio and SOD activity, but decrease the content of MDA. WP intervention increased some immune indicators. All the WP treated aging mice showed similar values of physiological indexes to that of the Vc group, even better. The relative abundance of Lactobacillus and Stenotrophomonas was increased and decreased, respectively, by both cow and goat WP. Lactobacillus may be involved in regulating the functional repair of organisms. In contrast, Stenotrophomonas might play a negative role in the immune and antioxidant capacity of the body. Combining physiological indicators and intestinal flora structure, low-concentration WP for cow and goat might be optimal for aging models.

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“…Lactobacillus species exerts protective effects on intestinal integrity and immune responses of the animal infected with Clostridium [25]. The improvement of anti-inflammatory status has been reported to be followed by the increase in the abundance of Prevotella [26]. Intestinal barrier injury is closely associated with numerous factors, such as bacterial infection, inflammation, and mechanical damage; all of which can be caused by Helicobacter and Escherichia_Shigella infection [27,28].…”

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confidence: 99%

Picroside II Improves Severe Acute Pancreatitis-Induced Intestinal Barrier Injury by Inactivating Oxidative and Inflammatory TLR4-Dependent PI3K/AKT/NF-κB Signaling and Improving Gut Microbiota

Piao¹,

Liu

Sui

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Picroside II exerts anti-inflammatory and antidiarrheal effects for treating the diseases associated with oxidative injury. However, its function on pancreatitis-induced intestinal barrier injury remains unclear. Hypothesis/Purpose. We hypothesized that picroside II will have protective effects against pancreatitis-induced intestinal barrier injury by affecting oxidative and inflammatory signaling (Toll-like receptor 4- (TLR4-) dependent phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), and nuclear factor kappa B (NF-κB)). Study Design and Methods. A Sprague-Dawley (SD) rat model with severe acute pancreatitis (SAP) was induced via the injection of sodium taurocholate (4% wt/vol; 1 mL/kg). All rats were divided into 3 groups: sham (CG), SAP-induced intestinal barrier injury (MG), and picroside II (PG) groups. Intestinal barrier injury was assessed by scanning electron microscopy (SEM), hematoxylin and eosin staining, and pathological scores. We measured the levels of pancreatitis biomarkers (amylase and lipase), oxidative and inflammatory signaling (TLR4-dependent PI3K/AKT/NF-κB), oxidative stress marker (superoxidase dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), and malondialdehyde), and inflammatory markers (tumor necrosis factor α (TNFα), interleukin- (IL-) 1, IL-6, and IL-10) in serum and/or gut tissues. Gut microbiota composition in feces was measured by using 16S rRNA sequencing. Results. SEM showed that intestinal barrier injury was caused with the loss of intestinal villi and mitochondria destruction, and pathological scores were increased in the MG group. The levels of amylase, lipase, malondialdehyde, TNFα, IL-1, IL-6, TLR4, PI3K, AKT, and NF-κB were increased, and the levels of SOD, GPx, CAT, and IL-10 was reduced in the MG group when compared with CG group (P<0.05). Picroside II treatment inhibited the symptoms in the MG group and showed antioxidant and anti-inflammatory activities. The serum levels of picroside II had strong correlation with the levels of inflammatory and oxidative stress biomarkers (P<0.05). Picroside II treatment increased the proportion of Lactobacillus and Prevotella and decreased the proportion of Helicobacter and Escherichia_Shigella in the model. Conclusions. Picroside II improved the SAP-induced intestinal barrier injury in the rat model by inactivating oxidant and inflammatory signaling and improving gut microbiota.

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Chronic oral exposure to glycated whey proteins increases survival of aged male NOD mice with autoimmune prostatitis by regulating the gut microbiome and anti-inflammatory responses

Abstract: Glycated whey proteins modulate the gut microbiome and are anti-inflammatory in aged male NOD mice.

Cited by 18 publications

References 51 publications

Association Of Gut Microbiota During Early Pregnancy With Risk Of Incident Gestational Diabetes Mellitus: A Nested Case-Control Study

Association Of Gut Microbiota During Early Pregnancy With Risk Of Incident Gestational Diabetes Mellitus: A Nested Case-Control Study

Effects of different types and doses of whey protein on the physiological and intestinal flora in D-galactose induced aging mice

Picroside II Improves Severe Acute Pancreatitis-Induced Intestinal Barrier Injury by Inactivating Oxidative and Inflammatory TLR4-Dependent PI3K/AKT/NF-κB Signaling and Improving Gut Microbiota

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