Chronic Rhinosinusitis with Nasal Polyps: A Proteomic Analysis

Upton, David C.; Welham, Nathan V.; Kuo, John S.; Walker, Jeffery W.; Pasic, Thomas R.

doi:10.1177/000348941112001203

Cited by 18 publications

(24 citation statements)

References 21 publications

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“…To the best of our knowledge only few investigations are present in literature about the NF protein composition in the turbinates . The greatest of specific literature is focused on NLF or nasal secretions and nasal epithelium or nasal mucosa . The inferior turbinate plays a pivotal role in the air conditioning activity of the nasal cavity (http://care.american-rhinologic.org/nasal_anatomy; last update: 01/20/2015).…”

Section: Resultsmentioning

confidence: 99%

A rapid differential display analysis of nasal swab fingerprints to distinguish allergic from non‐allergic rhinitis subjects by mesoporous silica particles and MALDI‐TOF mass spectrometry

et al. 2017

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Discriminating different rhinitis cases can sometimes be difficult as the diagnostic criteria used to identify the various subgroups are not always unambiguous. The nasal fluid (NF) highly reflects the pathophysiology of these inflammatory diseases. However, its collection, as nasal lavage fluid, may cause discomfort. Due to the non-invasiveness and rapidity of collection, nasal swab might represent an alternative to overcome these problems and also an ideal source of biomarkers. In this study, we demonstrate that the combined use of mesoporous silica (MPS) with MALDI-TOF MS allows the rapid detection of differential nasal peptide profiles from nasal swabs of healthy (H), allergic rhinitis (AR) and non-allergic rhinitis (NAR) subjects. NF peptides from nasal swabs were captured by the mean of MPS then profiled by MALDI-TOF MS. As a proof-of-principle, we also explored the ability of our platform to discriminate between nasal swabs of patients with AR and NAR, and between these groups and H controls. Four peaks resulted differentially expressed between NAR and AR, two peaks discriminated AR from H while one peak segregated NAR from H group. Therefore, peptides selected and enriched by our platform could form a part of a diagnostic ''rhinomic'' profile of the allergic and non-allergic patients.

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Section: Resultsmentioning

confidence: 99%

A rapid differential display analysis of nasal swab fingerprints to distinguish allergic from non‐allergic rhinitis subjects by mesoporous silica particles and MALDI‐TOF mass spectrometry

et al. 2017

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“…Other major barriers include the variability of classifying CRS subphenotypes, lack of cohort studies, heterogeneous environmental influences, and limited investment of research funding. Though more recent studies have attempted to search for genes related to sinus disease by use of DNA microarrays 14, 18-20 and protein expression analyses 20-22 , we still lack a clear understanding of the underlying molecular pathology. A fundamental problem is essentially all of the published studies have focused on CRS sufficiently advanced enough to require surgical intervention, making it difficult to determine the predisposing genetic and environmental factors that may affect the development of CRS from its onset.…”

Section: Introductionmentioning

confidence: 99%

“…Advantages of this strategy include: (1) an ability to examine variation in the human genome that predisposes to disease, thereby avoiding potentially confounding issues associated with CRS advanced enough to require surgery; (2) the opportunity to provide information on not only the mechanism but also the prognosis and response to therapy; and (3) direct applicability to human patients. Furthermore, this approach permits use of modern genetic methods toward greater insight into the disease biology, including: genome-wide association studies (GWAS), in which very large patient sample sizes are required to examine hundreds of thousands or millions of genetic variants to identify associations with disease 24 ; whole genome sequencing, which collects complete genetic information and therefore can detect rare genetic variants associated with disease 24 ; exome sequencing, in which only the coding portions of genes (exons) are sequenced 24 ; RNA sequencing, which explores how variation in RNA transcripts may correlate with disease 25 ; epigenomics, the study of how disease may be associated with biochemical modifications of DNA which affect gene regulation (e.g., DNA methylation or histone acetylation) 26 ; proteomics, the large scale analysis of the structure and function of proteins in cells or tissues 22 ; and metabolomics, the global analysis of small metabolites involved in cellular processes that may be affected by or predispose to disease. 27 One benefit from these unbiased approaches is that novel, clear, and testable hypotheses often emerge for subsequent direct evaluation.…”

Section: Introductionmentioning

confidence: 99%

Genetics of chronic rhinosinusitis: State of the field and directions forward

Hsu

Avila

Kern

et al. 2013

Journal of Allergy and Clinical Immunology

109

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The etiology of chronic rhinosinusitis (CRS) remains unclear. Study of the genetic susceptibility to CRS may be a valuable strategy to understand the pathogenesis of this burdensome disorder. The purpose of this review is to critically evaluate the current literature regarding the genetics of CRS in a comprehensive fashion. The most promising findings from candidate gene studies include the Cystic Fibrosis Transmembrane Conductance Regulator gene (CFTR), as well as genes involved in antigen presentation, innate and adaptive immune responses, tissue remodeling, and arachidonic acid metabolism. We also review the few hypothesis-independent genetic studies of CRS (i.e., linkage analysis and pooling-based genome-wide association studies). Interpretation of the current literature is limited by challenges with study design, sparse replication, few functional correlates of associated polymorphisms, and inadequate examination of linkage disequilibrium or expression quantitative trait loci for reported associations. Given the relationship of CRS to other airway disorders with well-characterized genetic components (e.g., asthma), study of the genetics of CRS deserves increased attention and investment, including the organization of large, detailed, and collaborative studies to advance knowledge of the mechanisms that underlie this disorder.

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“…S100A9 is overexpressed in nasal secretory cells from CRS patients with nasal polyps (28), as well as nasal mucus (29) and nasal lavage fluid from CRS patients (19). S100A8 is overexpressed in sinonasal mucosa tissue from patients with CRS with nasal polyps (30,31).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

et al. 2014

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Background: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins. Methods: Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings. results: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1. conclusion: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.

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Chronic Rhinosinusitis with Nasal Polyps: A Proteomic Analysis

Abstract: The proteomic approach allows detection of significant differences in protein abundance in CRSwNP and provides unique insight into the pathophysiology of this common disease.

Cited by 18 publications

References 21 publications

A rapid differential display analysis of nasal swab fingerprints to distinguish allergic from non‐allergic rhinitis subjects by mesoporous silica particles and MALDI‐TOF mass spectrometry

A rapid differential display analysis of nasal swab fingerprints to distinguish allergic from non‐allergic rhinitis subjects by mesoporous silica particles and MALDI‐TOF mass spectrometry

Genetics of chronic rhinosinusitis: State of the field and directions forward

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

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