2022
DOI: 10.1126/sciadv.abi7315
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Chronic superphysiologic AMH promotes premature luteinization of antral follicles in human ovarian xenografts

Abstract: Anti-Müllerian hormone (AMH) is produced by growing ovarian follicles and provides a diagnostic measure of reproductive reserve in women; however, the impact of AMH on folliculogenesis is poorly understood. We cotransplanted human ovarian cortex with control or AMH-expressing endothelial cells in immunocompromised mice and recovered antral follicles for purification and downstream single-cell RNA sequencing of granulosa and theca/stroma cell fractions. A total of 38 antral follicles were observed (19 control a… Show more

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Cited by 4 publications
(3 citation statements)
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“…There is no information on exogenous applications that affect AMH levels in cattle. Recent in vitro and in vivo experiments in humans and cats have shown that exogenously elevated AMH levels to superphysiological levels can contribute to ovulation disorders (Man et al, 2022) and lead to infertility (Vansandt et al, 2023), as is known for superphysiological AMH levels due to ganulosa cell tumours (Yucel Cicek et al, 2022). In this context, Vit D3 is an important candidate that may affect the AMH level because its effect on follicular development process.…”
Section: Discussionmentioning
confidence: 99%
“…There is no information on exogenous applications that affect AMH levels in cattle. Recent in vitro and in vivo experiments in humans and cats have shown that exogenously elevated AMH levels to superphysiological levels can contribute to ovulation disorders (Man et al, 2022) and lead to infertility (Vansandt et al, 2023), as is known for superphysiological AMH levels due to ganulosa cell tumours (Yucel Cicek et al, 2022). In this context, Vit D3 is an important candidate that may affect the AMH level because its effect on follicular development process.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, studies of antiMüllerian hormone (AMH, also known asmüllerian inhibiting substance, MIS) on follicle activation transcriptomic networks revealed that AMH inhibited the differentiation of pregranulosa cells to granulosa cells and the proliferation of epithelial and stromal cells in neonatal mice [84], highlighting the significance of the temporal link between germ cell and granulosa cell development [45, 84]. However, the administration in human of supraphysiologic AMH alone may cause ovulatory dysfunction by speeding antral follicle development and luteinization [85]. Titus performed scRNA-seq on primordial follicular oocytes and discovered that CTX enhanced the co-localization of BAD (BCL2 Associated Agonist Of Cell Death) and BCL2 (B-cell lymphoma 2), resulting in abrupt follicular loss via apoptosis and even POI [86].…”
Section: The Findings Of Single-cell Studies In Physiology Of Follicu...mentioning
confidence: 99%
“…Chronic high levels of AMH profoundly influence the antral-stage follicles, impacting the synthesis and metabolism of sex steroid hormones. 8 For the development of effective therapies for PCOS and its associated comorbidities, a deeper understanding of the pathophysiology of this syndrome is necessary, with the identification of neuroendocrine changes as well as structural and metabolic alterations in the hypothalamic neuronal and glial cells. Understanding the molecular pathways that AMH modulates could elucidate potential mechanistic contributions to PCOS.…”
mentioning
confidence: 99%