Chronocoulometric aptamer based assay for staphylococcal enterotoxin B by target-triggered assembly of nanostructured dendritic nucleic acids on a gold electrode

Chen, Xiaoye; Liu, Yun; Lu, Yichen; Xiong, Xiong; Li, Yi; Liu, Yuanjian; Xiong, Xianrong

doi:10.1007/s00604-019-3236-9

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…EIS aptasensors also have many applications in the risk assessment of toxins in food. Combining induced release strategy with amplification of HCR signals, Chen et al (2019) developed an electrochemically competitive nanoprobe for ultrasensitive specificity SEB detection measured with chronocoulometry. Three classical electrodes were used for this experiment, namely, a modified gold electrode, an Ag/AgCl reference electrode, and an auxiliary platinum wire electrode.…”

Section: Principles Of Aptasensors For S Aureus Detectionmentioning

confidence: 99%

“…Three classical electrodes were used for this experiment, namely, a modified gold electrode, an Ag/AgCl reference electrode, and an auxiliary platinum wire electrode. Under the first-rank conditions, the charge difference of SEB increased linearly with the logarithmic increase of SEB concentration in the range of 5 pg/ml to 100 ng/ml, and the detection limit was as low as 3 pg/ml ( Chen et al, 2019 ).…”

Section: Principles Of Aptasensors For S Aureus Detectionmentioning

confidence: 99%

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Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang

Yang

et al. 2021

Front. Microbiol.

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Staphylococcus aureus (S. aureus) is the top ordinary pathogen causing epidemic and food poisoning. The authentication of S. aureus has great significance for pathologic diagnosis and food hygiene supervision. Various biosensor methods have been established for identification. This paper reviews the research progress of aptasensors for S. aureus detection, focusing on the classification of aptamer technologies, including optical aptasensors and electrochemical aptasensors. Furthermore, the feasibility and future challenges of S. aureus detection for aptamer assays are discussed. Combining aptasensors with nanomaterials appears to be the developing trend in aptasensors.

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Section: Principles Of Aptasensors For S Aureus Detectionmentioning

confidence: 99%

Section: Principles Of Aptasensors For S Aureus Detectionmentioning

confidence: 99%

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang

Yang

et al. 2021

Front. Microbiol.

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“…Our previous work has been done on electrochemical detection of SEB based on DNA nanostructured probe. [26][27][28][29] DNA nanomaterials have excellent structural properties, but assembly is complex. [26][27][28] Impedance method is simple and convenient, but it is greatly disturbed by food substrate.…”

Section: Introductionmentioning

confidence: 99%

“…[26][27][28][29] DNA nanomaterials have excellent structural properties, but assembly is complex. [26][27][28] Impedance method is simple and convenient, but it is greatly disturbed by food substrate. 29 Thus, the purpose of this work is to develop a sensitive and easy aptamer-based electrochemical sensor, capable of reagentless detection of SEB with high sensitivity, selectivity, and extremely broad dynamic ranges.…”

Section: Introductionmentioning

confidence: 99%

Reagentless detection of staphylococcal enterotoxin B via electrochemical interrogation of conformational changes

Wang

Liu

2022

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An electrochemical biosensor for staphylococcal enterotoxin B (SEB) detection has been designed on the basis of electrochemical interrogation of conformational changes. Ferrocene-labeled hairpin probe (Fc-HP) and SEB aptamer are introduced for the construction of the platform. Without SEB, the rigid construction of DNA duplex that included SEB aptamer and Fc-HP prevented Fc getting access to the electrode surface, keeping the "eT-off" state in the detection system. In the presence of SEB, the interaction between SEB and the aptamer could trigger the disruption of DNA duplex and the restoration of hairpin structure, accompanied by the increase of Fc oxidation current. The decreasing distance between the redox probe and electrode upon the nucleic acid reconfiguration substantially increased the efficiency of eT, which resulted in the enhanced Fc signal. The proposed strategy presented a wide linear detection range from 0.005 to 100 ng mL À1 with a detection limit down to 3 pg mL À1 (S/N = 3). To investigate the applicability and reliability of the method in real food samples such as milk samples, we compared the results between this method and the commercial ELISA kit. The relative percentage error between the two assays ranged from À6.42% to 6.31%, indicating that there was no obvious difference between the results.

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“… 7 Aptamers showed the ability to bind to a vast range of molecular targets with high specificity and affinity, which makes them used for detecting SEB. 16 The combination of multiple nucleic acid aptamers can not only improve the affinity of specific binding target molecules, but also reduce the cost. Therefore, aptamers-based electrochemical technology has a potential application as bio-probes for bio-sensing application to detect due to its low cost, more stable, easier modification, easier synthesis, and higher affinity.…”

Section: Introductionmentioning

confidence: 99%