Chronological and biological aging of the human left ventricular myocardium: Analysis of microRNAs contribution

Ramos-Marquès, Estel; García-Mendívil, Laura; Pérez-Zabalza, María; Santander-Badules, Hazel; Srinivasan, Sabarathinam; Oliveros, Juan Carlos; Torres‐Pérez, Rafael; Cebollada, Alberto; Vallejo-Gil, José María; Fresneda-Roldán, Pedro Carlos; Fañanás-Mastral, Javier; Vázquez-Sancho, Manuel; Matamala-Adell, Marta; Sorribas-Berjón, Juan Fernando; Bellido-Morales, Javier André; Mancebón-Sierra, Francisco Javier; Vaca-Núñez, Alexánder Sebastián; Ballester-Cuenca, Carlos; Villaescusa, José Manuel; Garrido-Huéscar, Elisa; Segovia-Roldán, Margarita; Oliván-Viguera, Aida; Gómez-González, Carlos; Muñiz, Gorka; Diez, Emiliano; Ordovás, Laura; Pueyo, Esther

doi:10.1111/acel.13383

Cited by 8 publications

(7 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fibrosis is also a well-known arrhythmogenic substrate as it can hamper proper impulse propagation [ 4 ]. Recently, similar observations have been reported in humans and large animals (pig) in relation to age and fibrosis (amount and spatial distribution), and specifically related to collagen as chief component of the ventricular extracellular matrix [ 5 , 6 ].…”

Section: Introductionsupporting

confidence: 56%

“…This fibrosis impairs cardiac muscle relaxation and may result in heart failure. LV collagen deposition was reported to be higher in elderly individuals than in young individuals [ 3 , 5 , 51 ]. This increase in the degree of LV fibrosis was also shown to lead to ventricular dysfunction [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…This is a proof-of-concept study to demonstrate the utility of the developed methodology to underscore phenotypic changes present with age in a limited number of pig LV samples. Further characterization of the age-related cardiac remodeling in this model system of the human LV [ 5 ] would require a larger number of specimens. Of note, the reported procedure can be applied to study other cardiac pathologies related to collagen and sarcomeric alterations.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy

Bueno,

Martínez-Ojeda,

Pérez-Zabalza

et al. 2024

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Aging induces cardiac remodeling, resulting in an increase in the risk of suffering heart diseases, including heart failure. Collagen deposition increases with age and, together with sarcomeric changes in cardiomyocytes, may lead to ventricular stiffness. Multiphoton (MP) microscopy is a useful technique to visualize and detect variations in cardiac structures in a label free fashion. Here, we propose a method based on MP imaging (both two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) modalities) to explore and objectively quantify age-related structural differences in various components of cardiac tissues. Results in transmural porcine left ventricle (LV) sections reveal significant differences when comparing samples from young and old animals. Collagen and myosin SHG signals in old specimens are respectively 3.8x and >6-fold larger than in young ones. Differences in TPEF signals from cardiomyocyte were ∼3x. Moreover, the increased amount of collagen in old specimens results in a more organized pattern when compared to young LV tissues. Since changes in collagen and myosin are associated with cardiac dysfunction, the technique used herein might be a useful tool to accurately predict and measure changes associated with age-related myocardium fibrosis, tissue remodeling and sarcomeric alterations, with potential implications in preventing heart disease.

show abstract

Section: Introductionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy

Bueno,

Martínez-Ojeda,

Pérez-Zabalza

et al. 2024

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Aging is an important risk factor for ischemic heart disease and heart failure (62-64). Previous studies have identified diverse cell autonomous and nonautonomus pathways involved in regulation of cardiac and vascular aging processes (65)(66)(67)(68)(69). In vitro and in vivo studies, focusing on protective effects of PACAP against ischemic events, confirmed that PACAP confers significant anti-aging effects in the cardiovascular system (70).…”

Section: Ischemic Heart Disease and Heart Failurementioning

confidence: 88%

Role of endocrine PACAP in age-related diseases

et al. 2023

View full text Add to dashboard Cite

Pituitary adenylate cyclase activating polypeptide (PACAP) is a conserved neuropeptide, which confers diverse anti-aging endocrine and paracrine/autocrine effects, including anti-apoptotic, anti-inflammatory and antioxidant action. The results of the in vivo and in vitro experiments show that increasing emphasis is being placed on the diagnostic/prognostic biomarker potential of this neuropeptide in a wide array of age-related diseases. After the initial findings regarding the presence and alteration of PACAP in different body fluids in physiological processes, an increasing number of studies have focused on the changes of its levels in various pathological conditions associated with advanced aging. Until 2016 – when the results of previous human studies were reviewed – a vast majority of the studies had dealt with age-related neurological diseases, like cerebrovascular and neurodegenerative diseases, multiple sclerosis, as well as some other common diseases in elderly such as migraine, traumatic brain injury and post-traumatic stress disorder, chronic hepatitis and nephrotic syndrome. The aim of this review is to summarize the old and the new results and highlight those ‘classical’ and emerging clinical fields in which PACAP may become subject to further investigation as a diagnostic and/or prognostic biomarker in age-related diseases.

show abstract

“…Heart aging can be triggered by a variety of complex factors, including the decline of stem cell functionality and quantity [11] , cardiomyocyte senescence [12] , DNA damage [13] , overproduction of reactive oxygen species (ROS) [14][15] , and telomere shortening [13] , as well as the activation of aging genes [6][7] . Some molecules and signaling pathways have also been found to be involved in the regulation of heart aging [16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Altered expression profile of long non-coding RNAs during heart aging in mice

Wang

Hua

et al. 2022

Frigid Zone Medicine

View full text Add to dashboard Cite

Objective Long noncoding RNAs (lncRNAs) play an important role in regulating the occurrence and development of cardiovascular diseases. However, the role of lncRNAs in heart aging remains poorly understood. The objective of this study was to identify differentially expressed lncRNAs in the heart of aging mice and elucidate the relevant regulatory pathways of cardiac aging. Materials and methods Echocardiography was used to detect the cardiac function of 18-months (aged) and 3-months (young) old C57BL/6 mice. Microarray analysis was performed to unravel the expression profiles of lncRNAs and mRNAs, and qRT-PCR to verify the highly dysregulated lncRNAs. Results Our results demonstrated that the heart function in aged mice was impaired relative to young ones. Microarray results showed that 155 lncRNAs were upregulated and 37 were downregulated, and 170 mRNAs were significantly upregulated and 44 were remarkably downregulated in aging hearts. Gene ontology analysis indicated that differentially expressed genes are mainly related to immune function, cell proliferation, copper ion response, and cellular cation homeostasis. KEGG pathway analysis showed that the differentially expressed mRNAs are related to cytokine-cytokine receptor interaction, inflammatory mediator regulation of TRP channels, and the NF-kappa B signaling pathway. Conclusion These results imply that the differentially expressed lncRNAs may regulate the development of heart aging. This study provides a new perspective on the potential effects and mechanisms of lncRNAs in heart aging.

show abstract

Chronological and biological aging of the human left ventricular myocardium: Analysis of microRNAs contribution

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 66 publications

Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy

Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy

Role of endocrine PACAP in age-related diseases

Altered expression profile of long non-coding RNAs during heart aging in mice

Contact Info

Product

Resources

About