Chrysanthemum stunt: A viroid disease

Diener, T.O.; Lawson, R. H.

doi:10.1016/0042-6822(73)90369-3

Cited by 107 publications

(29 citation statements)

References 14 publications

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“…CSVd causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Typical reactions in CSVd-infected chrysanthemum cultivars are stunting with a reduction of one-half to two-thirds relative to the normal plant height and flower color bleaching (Bouwen and Annemarie, 1995; Diener and Lawson, 1973; Hooftman et al, 1996; Horst and Nelson, 1997). The affected plants are often unproductive and the causal viroid is easily transmitted by foliar contact, cultivation practices and cutting knives.…”

mentioning

confidence: 99%

Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

Yoon

Cho²,

Choi³

et al. 2014

The Plant Pathology Journal

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Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK1 could infect systemically tomato seedlings and chrysanthemum plants, suggesting that the linear CSVd RNA transcribed from the cDNA clones could be replicated as efficiently as circular CSVd in host species. However, direct inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants.

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mentioning

confidence: 99%

Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

Yoon

Cho²,

Choi³

et al. 2014

The Plant Pathology Journal

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“…Natural infections of CSVd have been reported for Ageratum (Henkel and Sanger 1995) Dendranthema (chrysanthemum) (Dimock 1947;Diener and Lawson 1973) and in Dahlia bulbs in Japan. CSVd was found in 77.2% of the tested plants.…”

Section: Discussionmentioning

confidence: 95%

“…CSVd, the causal agent of chrysanthemum stunt disease, is an infectious single stranded circular RNA with a chain length of 354 or 356 nucleotides (Diener and Lawson 1973;Hollings and Stone 1973;Haseloff and Symons 1981;Gross et al 1982). The magnitude of CSVd infection of chrysanthemum plants varies between cultivars.…”

Section: Introductionmentioning

confidence: 99%

Screening of chrysanthemum cultivars forChrysanthemum stunt viroidin an Indian scenario

Singh

Pathania

Ram

et al. 2010

Archives Of Phytopathology And Plant Protection

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Chrysanthemum stunt viroid (CSVd) belonging to Pospiviroidae was found prevailing in some of the chrysanthemum plant samples collected from various geographical regions of India. In this study 70% cultivars of chrysanthemum were tested positive for CSVd by reverse transcription polymerase chain reaction (RT-PCR) and DNA-RNA hybridisation. The viroid was found to be mechanically transmissible to in vitro grown healthy chrysanthemum cv. Regol Time. The RT-PCR amplified product of CSVd was sequenced (348 bp length) and was directly used as a probe for hybridisation. Isolates from Palampur, Mandi, Karnataka, Bangalore and Sikkim showed positive results for CSVd. This analysis shows that CSVd is prevailing in these regions of India.

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“…Chrysanthemum stunt disease was reported in the early 1950s [17]. It causes light green young leaves, stunting, small leaves and flowers, and reduced rooting ability.…”

Section: Symptoms Isolation Nucleotide Sequences and Structuresmentioning

confidence: 99%

A Current Overview of Two Viroids That Infect Chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid

Cho

et al. 2013

Viruses

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The chrysanthemum (Dendranthema X grandiflorum) belongs to the family Asteraceae and it is one of the most popular flowers in the world. Viroids are the smallest known plant pathogens. They consist of a circular, single-stranded RNA, which does not encode a protein. Chrysanthemums are a common host for two different viroids, the Chrysanthemum stunt viroid (CSVd) and the Chrysanthemum chlorotic mottle viroid (CChMVd). These viroids are quite different from each other in structure and function. Here, we reviewed research associated with CSVd and CChMVd that covered disease symptoms, identification, host range, nucleotide sequences, phylogenetic relationships, structures, replication mechanisms, symptom determinants, detection methods, viroid elimination, and development of viroid resistant chrysanthemums, among other studies. We propose that the chrysanthemum and these two viroids represent convenient genetic resources for host–viroid interaction studies.

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Chrysanthemum stunt: A viroid disease

Cited by 107 publications

References 14 publications

Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

Screening of chrysanthemum cultivars forChrysanthemum stunt viroidin an Indian scenario

A Current Overview of Two Viroids That Infect Chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid

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