Screening of chrysanthemum cultivars forChrysanthemum stunt viroidin an Indian scenario

Abstract

Chrysanthemum stunt viroid (CSVd) belonging to Pospiviroidae was found prevailing in some of the chrysanthemum plant samples collected from various geographical regions of India. In this study 70% cultivars of chrysanthemum were tested positive for CSVd by reverse transcription polymerase chain reaction (RT-PCR) and DNA-RNA hybridisation. The viroid was found to be mechanically transmissible to in vitro grown healthy chrysanthemum cv. Regol Time. The RT-PCR amplified product of CSVd was sequenced (348 bp length) and was directly used as a probe for hybridisation. Isolates from Palampur, Mandi, Karnataka, Bangalore and Sikkim showed positive results for CSVd. This analysis shows that CSVd is prevailing in these regions of India.

Phylogenetic analysis and molecular characterization of chrysanthemum chlorotic mottle viroid and chrysanthemum stunt viroid from chrysanthemum in Thailand

“…CSVd infection rates were high in South Korea with the incidence up to 100% (Chung et al, 2005), 90% in Japan (Matsushita et al, 2007), 70% in India (Singh et al, 2010) and 12% in China (Zhang et al, 2011). In Thailand, CSVd was first found with 34.78% incidence (8 positive samples from all 23 collected samples) (Netwong et al, 2020), while in this study CSVd incidence was quite low with 3.33% (5/150 samples).…”

A Current Overview of Two Viroids That Infect Chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid

“…Also, a linear CSVd RNA, synthesized by in vitro transcription, could infect chrysanthemum plants and other plants that belong to the families Solanaceae and Asteraceae , without causing any disease symptoms [33]. Under natural conditions, infections of CSVd have been reported in Petunia hybrida [34], Ageratum , Dahlia [35], and Senecio (cineraria) [36]. CSVd was also identified in Vinca major with reverse transcriptase (RT)-PCR [37].…”

“…In 2010, 13 samples with mild chlorotic spots were collected, and all were infected with CChMVd [55]. In India, chrysanthemum cultivars were screened for CSVd infections with RT-PCR and DNA-RNA hybridization: 70% of cultivars were infected with CSVd [36]. …”