Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant

Abstract

Yellow-pigmented, Gram-negative organisms isolated from raw chicken were investigated by means of a polyphasic taxonomic approach and were shown to represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium vrystaatense sp. nov. is proposed. Its nearest phylogenetic neighbours were Chryseobacterium joostei, Chryseobacterium indologenes and Chryseobacterium gleum, which showed 16S rRNA gene sequence similarity levels of 96·9, 97·1 and 96·1 %, respectively. Levels of DNA–DNA hybridization between strains of C. vrystaatense and Chryseobacterium reference species were below 46 %. Strain LMG 22846T (=CCUG 50970T) was chosen as the type strain and has a DNA G+C content of 37·1 mol%.

Chryseobacterium hungaricum sp. nov., isolated from hydrocarbon-contaminated soil

“…Phenotypic characteristics that differentiate strain CHB-20p T from other species of the genus Chryseobacterium Species/strains: 1, C. hungaricum sp. nov. CHB-20p T (data from this study); 2, C. caeni N4 T (Quan et al, 2007); 3, C. gleum (n52; Vandamme et al, 1994); 4, C. balustinum ATCC 33487 T (Vandamme et al, 1994); 5, C. indologenes (n57; Vandamme et al, 1994); 6, C. indoltheticum LMG 4025 T (Vandamme et al, 1994; this study); 7, C. scophthalmum (n52; Vandamme et al, 1994; this study); 8, C. defluvii B2 T (Kämpfer et al, 2003; this study); 9, C. joostei (n511; Hugo et al, 2003); 10, C. daecheongense CPW406 T (Kim et al, 2005a; this study); 11, C. formosense CC-H3-2 T ; 12, C. taichungense CC-TWGS1-8 T ; 13, C. shigense GUM-Kaji T (Shimomura et al, 2005);14, C. vrystaatense (n536;de Beer et al, 2005); 15, C. soldanellicola PSD1-4 T (Park et al, 2006); 16, C. taeanense PHA3-4 T (Park et al, 2006);17, C. piscium (n54;de Beer et al, 2006); 18, C. hispanicum VP48 T (Gallego et al, 2006); 19, C. wanjuense R2A10-2 T (Weon et al, 2006) Table 2. Cellular fatty acid composition of Chryseobacterium species Species/strains: 1, C. hungaricum sp.…”

“…Cellular fatty acid composition of Chryseobacterium species Species/strains: 1, C. hungaricum sp. nov. CHB-20p T (data from this study); 2, C. caeni N4 T (Quan et al, 2007); 3, C. taichungense CC-TWGS1-8 T ; 4, C. formosense CC-H3-2 T ; 5, C. defluvii B2 T (Kämpfer et al, 2003); 6, C. joostei (n511; Hugo et al, 2003); 7, C. gleum (n55; Vandamme et al, 1994); 8, C. indologenes (n545; Vandamme et al, 1994); 9, C. balustinum ATCC 33487 T (Vandamme et al, 1994); 10, C. indoltheticum LMG 4025 T (Vandamme et al, 1994); 11, C. scophthalmum (n52; Vandamme et al, 1994);12, C. vrystaatense (n57;de Beer et al, 2005); 13, C. daecheongense CPW406 T (Kim et al, 2005a); 14, C. shigense GUM-Kaji T (Shimomura et al, 2005), 15, C. soldanellicola PSD1-4 T (Park et al, 2006); 16, C. taeanense PHA3-4 T (Park et al, 2006);17, C. piscium (n54;de Beer et al, 2006); 18, C. hispanicum VP48 T (Gallego et al, 2006); 19, C. wanjuense R2A10-2 T (Weon et al, 2006). Fatty acid percentages amounting to less than 1 % of the total fatty acids in all strains were not included.…”

Chryseobacterium camelliae sp. nov., isolated from green tea

“…The membership of the genus has been growing rapidly, as demonstrated by the multitude of novel species that have been proposed in recent years. At the time of writing, the genus Chryseobacterium contains 72 species isolated from various environments such as soils (Weon et al, 2008), fresh water (Strahan et al, 2011), sewage and wastewater (Kämpfer et al, 2003), compost (Kämpfer et al, 2010), the plant rhizosphere (Park et al, 2006), diseased fish (Bernardet et al, 2005), meat (de Beer et al, 2005) and dairy environments (Hugo et al, 2003). In this study, we report the taxonomic characterization of a novel member of the genus Chryseobacterium, strain THG C4-1 T .…”

Chryseobacterium carnipullorum sp. nov., isolated from raw chicken

“…The type species is C. gleum. Members of the genus Chryseobacterium include psychrotolerant and proteolytic spoilage micro-organisms that are widely distributed in food sources such as milk, fish, meat and poultry (Hugo et al, 2003;de Beer et al, 2005 de Beer et al, , 2006 and occur in a variety of ecological niches such as soil, water, sludge, plants, and human and fish clinical specimens (Hugo et al, 2003;Bernardet et al, 2005Bernardet et al, , 2011Hugo & Jooste, 2012).In 2006 the genus Chryseobacterium consisted of only 10 validly named species. Since then the genus has undergone significant and rapid expansion and, at the time of writing, it comprises 61 species with validly published names (Bernardet et al, 2006) and more continue to be described (Euzéby, 2012).…”