Chrysin enhances antioxidants and oxidative stress in L-NAME-induced hypertensive rats

Abstract

Objectives:The study seeks to evaluate the effect of chrysin; a natural, biologically active compound extracted from plants, honey or propolis, on the tissues and circulatory antioxidant status and lipid peroxidation in N ω -nitro-l-arginine methyl ester (L-NAME) induced hypertensive rats. Materials and Methods: Male albino rats were divided into four groups. Control (Group I) and chrysin supplementation of the control (Group II) received normal diet. Groups III and IV received L-NAME (40 mg/kg B.W). Groups II and IV received chrysin (25 mg/kg B.W) dissolved in 0.2% dimethylsulfoxide solution after the 4 th week. Results and Discussion: The results showed significantly elevated levels of tissue and circulatory thiobarbituric acid reactive substances, conjugated dienes and lipid hydroperoxides, and significantly lowered enzymic and non-enzymic antioxidant activity of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C and vitamin E in L-NAME-induced hypertensive rats compared with those in control group. From chrysin administration to rats with L-NAME-induced hypertension leads to tissue damage which significantly decreases the levels of thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes, and significantly elevates the activity of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C and vitamin E in the tissues and circulation compared with those on the unsupplemented L-NAME induced hypertensive group. Conclusions: Chrysin offers protection against free radical-mediated oxidative stress in rats with L-NAME-induced hypertension.

Effect of Hydroalcoholic Extract of Ribes khorasanicum on Acute Hypertension Induced by L-NAME in Rat

“…Inhibition of NO production can increase blood pressure, which is accompanied by increased free radicals and oxidative stress [16, 17]. In line with our results, previous studies have shown that the antioxidant activity of superoxide dismutase, catalase, and glutathione peroxidase has decreased in the L-NAME administrated rats [17]. …”

Chrysin Pretreatment Improves Angiotensin System, cGMP Concentration in L-NAME Induced Hypertensive Rats

“…Reactive oxygen species (ROS) also have been shown to be critical determinants in hypertension. The contribution of oxidative stress to the pathogenesis of hypertension is supported to rely upon inactivation of the NO [3]. Renin–angiotensin system (RAS) plays a vital role in the regulation of vascular function and blood pressure [4].…”

“…At the same time chrysin has been found to posses tyrosinase inhibitory activity and moderate aromatase inhibitory activity. It can also inhibit estradiol-induced DNA synthesis [3]. A number of its derivatives have been prepared, which helps in improving the biological activity of chrysin [14].…”

Current insights and future perspectives of flavonoids: A promising antihypertensive approach

“…At the same time, moreover, chrysin has been shown to have tyrosinase inhibitory activity as well as mild aromatase inhibitory activity. It can also prevent estradiol‐stimulated DNA synthesis (Veerappan & Senthilkumar, 2015). Chrysin has antihypertensive properties, reducing hepatic, renal, and endothelial dysfunction in rodents with L‐NAME‐induced hypertension and inhibits the increase in superoxide and hydroxyl free radicals in enzymatic and nonenzymatic systems (Adjoke et al, 2019; Veerappan & Malarvili, 2019).…”