Chrysin Pretreatment Improves Angiotensin System, cGMP Concentration in L-NAME Induced Hypertensive Rats

Abstract

N ω -nitro- l -arginine methyl ester (L-NAME) is a non-specific nitric oxide (NO) synthase inhibitor, commonly used for the induction of NO-deficient hypertension. The objective of the present study was to investigate the effects of chrysin with flavnoids, on L-NAME-induced hypertensive rats. Methods: An experimental hypertensive animal (180–220 g) model was induced by L-NAME intake on rats. In treatment chrysin was orally administered 25 mg/kg body weight (b.w.). Blood pressure was measured by tail cuff plethysmography system. Cardiac and vascular function was evaluated by Langendorff isolated heart system with Angiotensin II (Ang-II), Hexo oxygenase (HO-1), cyclic guanosine monophosphate (cGMP) concentration in tissues respectively. Rats with hypertension showed an elevated blood pressure (BP), left ventricular functions, ang II, and decreased cGMP concentration of tissues. Treatment of chrysin is reverse to near normal in left ventricular functions, Ang-II, Ho-1 and decreased cGMP concentration of tissues. The antihypertensive effect of chrysin appears to be mediated by a reduction in left ventricular functions, cardiac oxidative stress and Ang-II, an increase in cardiac HO-1, cGMP concentration and a prevention of plasma nitric oxide loss.

UHPLC-DAD characterization of bioactive secondary metabolites from Ocimum americanum and Pupalia lappacea extracts: Antioxidant activity and antihypertensive effects on L-NAME-induced hypertensive rats

“…Chrysin have been showed to possess an antihypertensive effect by lowering blood pressure, lipid peroxides and improved antioxidant status. The suggested pharmacological mechanism is that chrysin inhibits production of superoxide and hydroxyl free radicals in enzymatic and nonenzymatic systems (Veerappan and Thekkumalai, 2018). Rutin identied in extract was showed to increase NO production in human endothelial cells and improved endothelial functions (Ugusman et al, 2014;Aditya and Ajay, 2017).…”

In vitro and in vivo activities of flavonoids – apigenin, baicalin, chrysin, scutellarin – in regulation of hypertension – a review for their possible effects in pregnancy-induced hypertension

“…Chrysin decreased also proliferation of smooth muscle cells in the vascular media of small pulmonary arteries [91]. Veerappan and Malarvili [90] observed that chrysin (25 mg/kg/day for 4 weeks, p.o.) administered to rats with hypertension caused by N-nitro-Larginine methyl ester showed antihypertensive effect throughout the following mechanism of action such as: reduction in functions of left ventricular, oxidative stress in cardiac tissue and level of angiotensin II in plasma, and increasing of hexooxygenase in cardiac tissue, concentration of cGMP and a prevention of plasma nitric oxide loss.…”

“…Moreover, chrysin was marked in herbs of Scutellaria galericulata L. [4], and Passilora caerulea L. [86,87]. Chrysin showed various pharmacological effects, such as anti-inflammatory [88], antioxidant [89], and antihypertensive activity [86,90,91,95], neuroprotective [86], anticancer properties [86], possessed protective effects against toxic agents [92], and many others [86].…”

The Cardiovascular Protective Effects of Chrysin: A Narrative Review on Experimental Researches

“…The findings indicated that chrysin could ameliorate vascular relaxation by activating cyclic guanosine monophosphate (cGMP) pathway. cGMP reduces Ca 2+ sensitivity of contractile components via activation of cGMP-kinase [51]. cGMP stimulates the protein kinase G, thereby modulates vascular smooth muscle cell relaxation.…”