Chrysin protects mice from Staphylococcus aureus pneumonia

Abstract

Aim: To elucidate the effect of chrysin on α‐haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. Methods and Results: Haemolysis, Western blot and real‐time RT‐PCR assays were performed to evaluate the effect of chrysin on α‐haemolysin secretion by Staph. aureus. The efficacy of chrysin against human alveolar epithelial cell injury by α‐haemolysin was tested using live/dead staining or by measuring lactate dehydrogenase activity. Furthermore, we determined the protective effect of chrysin against Staph. aureus pneumonia through histopathology experiments in a mouse model. The production of α‐haemolysin by Staph. aureus was inhibited when presented with an increasing subinhibitory concentration of chrysin in vitro. Consistent with this result, chrysin prevented α‐haemolysin‐mediated cell injury and protected mice from Staph. aureus pneumonia. Conclusions: Chrysin is a potent inhibitor of α‐haemolysin expression by Staph. aureus, and it conferred a significant degree of protection against Staph. aureus pneumonia. Significance and Impact of Study: The chrysin‐mediated inhibition of α‐haemolysin production and protection against Staph. aureus pneumonia may offer a new strategy in combating pathogen infections.

Flavone Reduces the Production of Virulence Factors, Staphyloxanthin and α-Hemolysin, in Staphylococcus aureus

“…Recently, the flavonoids luteolin [19] and chrysin [23] at subinhibitory concentrations showed an ability to inhibit the hemolysis of S. aureus, and fisetin reduced the antibioticresistant biofilm formation in S. aureus [5], which demonstrated the potential antivirulence activity of these flavonoids. Compared with luteolin, chrysin, and fisetin, flavone specifically reduced the virulence factor of staphyloxanthin and the H 2 O 2 resistance.…”

“…Recently, several plant compounds have been reported to decrease the virulence of S. aureus without affecting its growth. For example, thymol found in thyme [20] reduced enterotoxins and ahemolysin production; luteolin [19] and chrysin [23] reduced a-hemolysin production; and fisetin [5] and olelic acid [22] inhibited the biofilm formation in S. aureus.…”

Diosmetin inhibits the expression of alpha-hemolysin in Staphylococcus aureus

“…Previous studies have shown that Hla produced by S. aureus could cause the hemolysis of rabbit erythrocytes (Wang et al 2011). Therefore, a hemolytic activity assay was employed to examine the hemolytic activity of S. aureus culture supernatants in the presence of varying concentrations of diosmetin.…”

Development of a new certified reference material of high-purity chrysin for the quality control of traditional Chinese medicine

“…1. Literature reports have demonstrated that chrysin possesses multiple biological activities such as antioxidant [5,6], anti-inflammatory [7], antibacterial [8], antiviral [9], hypolipidemic [10], antihypertensive [11], anti-diabetic [12], anxiolytic [13,14], anti-allergic [15], hepatoprotective [16], and anti-tumour effects. The tumour prevention and treatment effects of chrysin have attracted more and more attention in recent years.…”