Cigarette smoke inhalation increases the alveolar bone loss caused by primary occlusal trauma in a rat model

Campos, Mirella Lindoso Gomes; Corrêa, Mônica Grazieli; Júnior, Francisco Humberto Nociti; Casati, Márcio Z.; Sallum, Enílson Antônio; Sallum, Antônio Wilson

doi:10.1111/jre.12091

Cited by 20 publications

(20 citation statements)

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“…There are many local contributing factors that accelerate the bone loss process, such as dental calculus, bacterial factors, and occlusal trauma. Occlusal trauma is defined as an injury to the periodontium caused by traumatic force that exceeds the reparative capacity of periodontal tissues . It has been suggested that occlusal trauma may result from questionable restoration; bruxism or clenching that alters the force applied to teeth.…”

Section: Introductionmentioning

confidence: 99%

Occlusal trauma inhibits osteoblast differentiation and bone formation through IKK‐NF‐κB signaling

Lü

Yue

et al. 2019

Journal of Periodontology

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Background:Occlusal trauma is an important factor promoting bone loss caused by periodontal diseases. Although there are reports of traumatic force promoting bone resorption in periodontal diseases, no studies examining the inhibition of bone formation by traumatic force and the underlying mechanism have been reported. The aim of this study was to investigate the mechanism whereby traumatic force inhibits bone formation.Methods: MC3T3-E1 cells were induced to undergo osteogenic differentiation and subjected to cyclic uniaxial compressive stress with or without stimulation with Pg. LPS. The expression of osteoblast markers and the activation of IKK-NF-B signaling were evaluated in vitro. Then, MC3T3-E1 cells were induced to undergo osteogenic differentiation and subjected to cyclic uniaxial compressive stress with or without IKK-2 Inhibitor VI. The expression of osteoblast markers was determined. Then, the classic Wnt signaling pathway ( -catenin, Gsk3 , p-Gsk3 , and Dkk1) was further evaluated in vitro. Finally, occlusal trauma was induced in Wistar rats with or without the injection of IKK-2 Inhibitor VI, to evaluate changes in bone mass and IKK-NF-B and Wnt/ -catenin signaling in vivo. Results:After stimulation with Pg. LPS and traumatic force, IKK-NF-B signaling was significantly activated in vitro. The expression of osteoblast markers and the activity of alkaline phosphatase in MC3T3-E1 cells declined after traumatic force loading and were rescued when IKK-NF-B signaling was blocked. Wnt/ -catenin signaling was accordingly inhibited upon force loading, but this inhibition was reversed when IKK-NF-B was antagonized in vitro. X-ray and Micro-CT analysis of the mandibles of the rats as well as HE and TRAP staining showed that bone loss induced by occlusal trauma declined after IKK-NF-B was inhibited. The expression of p65 and I B was increased when occlusal trauma was induced in Wistar rats, whereas -catenin, OCN, and Runx2 levels were decreased. After J Periodontol. 2020;91:683-692.

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Section: Introductionmentioning

confidence: 99%

Occlusal trauma inhibits osteoblast differentiation and bone formation through IKK‐NF‐κB signaling

Lü

Yue

et al. 2019

Journal of Periodontology

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“…It is known that there is alveolar bone loss in occlusal trauma 1,8,15 , because all periodontal support tissues are involved. However, there is no information in the literature about the type of occlusal condition and its pathogenic intensity in the periodontium.…”

Section: Discussionmentioning

confidence: 99%

“…Due to ethical limitations on prospective evaluation of OT in humans, 2 experimental models in rats were developed for the present study, with the goal of evaluating the consequence of the type of occlusion on the development of bone loss in primary OT. In one of the models, occlusal interference 7,8,11 was created to imitate experimentally the occlusal interferences and premature occlusal contacts that lead to the increase in the vertical dimension of occlusion, leading to occlusal instability by altering the force vectors and changing the magnitude of the applied forces to one tooth. In the other model, the unilateral vertical dimension was reduced, creating occlusion on only one of the first molars, with the goal of reproducing experimentally the cases of partial edentulism 15 , also creating occlusal overload experimentally.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have been conducted with the aim of understanding the cellular, molecular and genetic mechanisms involved in occlusal trauma [7][8][9][10][11] . Clinically, however, there is an ethical implication that limits the conduct of prospective studies on occlusal trauma in humans, making it difficult to measure occlusal overload and its implications on bone, cementum and the periodontal ligament 12,13 .…”

Section: Introductionmentioning

confidence: 99%

“…For this reason, experimental studies are frequently conducted using animal models to evaluate the effect of occlusal forces on the periodontium. There are studies of occlusal trauma that have been developed satisfactorily by constructing occlusal apparatus that led to occlusal interference 8,14 or occlusal overload, experimentally mimicking partially edentulous patients [15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of bone loss due to primary occlusal trauma in two experimental models of occlusal overload

Lopes

Téo

Corrêa

et al. 2016

Rev. odontol. UNESP

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ResumoIntrodução: Trauma oclusal primário (TO) é a injúria causada no periodonto de inserção de dentes com periodonto de altura normal devido a forças oclusais que excedem sua capacidade adaptativa. Objetivo: Avaliar histometricamente a reabsorção óssea alveolar na região da furca do 1º molar inferior em ratos submetidos experimentalmente a 2 modelos de sobrecarga oclusal. Material e método: 45 animais aleatoriamente divididos em 3 Grupos: Interferência Oclusal (GI, n = 15) -fixação de um segmento de fio ortodôntico na face oclusal do 1º molar inferior; Sobrecarga Oclusal (GS, n = 15) -desgaste das cúspides dos molares inferiores contralaterais e dos 2º e 3º molares do lado do 1º molar que teve suas dimensões mantidas; Grupo Controle Negativo (CN, n = 15) -avaliação das dimensões iniciais do ligamento periodontal. Cinco animais/grupo foram submetidos à eutanásia após 14, 21 e 28 dias. Resultado: A avaliação intergrupos mostrou que houve reabsorção óssea significativa em GI (p<0,001) e GS (p<0,01) quando comparados com CN. O grupo GI teve reabsorção óssea significativamente maior quando comparado ao grupo GS em 14 (p<0,01), 21 (p<0,01) e 28 dias (p<0,01). A avaliação intragrupos não mostrou influência significativa do tempo na reabsorção óssea em GI e GS, independente da técnica utilizada (p>0,05). A espessura do ligamento periodontal manteve-se estável no grupo CN (p>0,05). Conclusão: GI e GS foram eficazes na reprodução experimental do TO e GI promoveu maior reabsorção óssea alveolar quando comparado a GS, mostrando que o impacto causado pela sobrecarga oclusal no grupo GI aumentou a lesão por TO.Descritores: Reabsorção óssea; oclusão dental; ferimentos e lesões; ratos. AbstractIntroduction: Primary occlusal trauma (OT) is an injury of the periodontium with normal height as a result of occlusal forces which exceed their adaptive capacity. Objective: To evaluate, histometrically, the alveolar bone loss in the furcation region of rats experimentally submitted to 2 models of occlusal overload. Material and method: 45 animals randomly divided into 3 groups: Occlusal Interference (OI, n = 15) -fixing an orthodontic wire segment on the occlusal surface of the first lower molar; Occlusal Overload (OO, n = 15) -wearing of the cusps of the lower contralateral molars, the second and third molars next to the first molar that had its dimensions maintained; Negative Control (NC, n = 15) -evaluation of the initial dimensions of the periodontal ligament (PL). Five animals / group were sacrificed after 14, 21 and 28 days. Result: Intergroup evaluation showed significant bone loss in OI (p<0.001) and OO (p<0.01) compared to NC. OI had significantly higher bone loss compared to OO at 14 (p<0.01), 21 (p <0.01) and 28 days (p<0.01). The intragroup evaluation showed no significant influence of time on bone loss in OI and OO, regardless of the technique used (p>0.05). The thickness of the PL remained stable in NC (p>0.05). Conclusion: OI and OO were effective in the experimental reproduction of OT, and OI promoted greater alveolar b...

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Traumatic compressive stress inhibits osteoblast differentiation through long chain non‐coding RNA Dancr

Lü

Liu

et al. 2020

Journal of Periodontology

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Background: Occlusal trauma is an important local contributing factor aggravating periodontal pocket and alveolar bone absorption in periodontal diseases. Our previous studies have found that occlusal trauma inhibited osteogenic differentiation through nuclear factor (NF)-B signaling. To further investigate the underlying mechanism, the aim of this study was to explore the role of long chain non-coding differentiation antagonizing non-protein coding RNA (Dancr) in the inhibitory effect of traumatic stress on osteoblast differentiation. Methods: We took the MC3T3-E1 cells as object in vitro research and stimulated cells with simple stress load, Dancr-siRNA + stress load, Dancr overexpression-plasmid + stress load. Quantitative real-time polymerase chain reaction was used to detect the RNA expression levels of Dancr, alkaline phosphatase (Alp) and Runt-related transcription factor 2 (Runx2). The protein expressions of Alp and Runx2 were tested by Western blot and the activity of Alp was qualitatively demonstrated by Alp staining. In addition, Western blot was performed to investigate the role of Dancr in affecting NF-B signaling pathway. Results: Traumatic compressive stress inhibited the expressions of Alp, Runx2, and-Dancr in MC3T3-E1 cells. Stress-induced inhibition of osteoblast differentiation was promoted after silencing Dancr. Overexpression of Dancr could alleviate the inhibitory effect of traumatic force on osteoblast differentiation to some extent. Furthermore, NF-B signaling was activated after silencing Dancr, and the activated effect of traumatic force on NF-B signaling could be alleviated through overexpression of Dancr to some extent. Conclusion: Traumatic compressive stress can indirectly activate the NF-B signaling through downregulation of Dancr, thereby inhibiting osteogenic differentiation.

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Cigarette smoke inhalation increases the alveolar bone loss caused by primary occlusal trauma in a rat model

Abstract: Short-term CSI increases early bone loss in association with OT after 7 d, and this worsens in severity after 14 d of exposure.

Cited by 20 publications

References 40 publications

Occlusal trauma inhibits osteoblast differentiation and bone formation through IKK‐NF‐κB signaling

Occlusal trauma inhibits osteoblast differentiation and bone formation through IKK‐NF‐κB signaling

Evaluation of bone loss due to primary occlusal trauma in two experimental models of occlusal overload

Traumatic compressive stress inhibits osteoblast differentiation through long chain non‐coding RNA Dancr

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