Ciliary Beating Plane and Wave Propagation in the Bovine Oviduct

Schätz, G.; Schneiter, Martin; Rička, J.; Kühni-Boghenbor, K.; Tschanz, Stefan A.; Doherr, Marcus G.; Frenz, Martin; Stoffel, Michael

doi:10.1159/000360155

Cited by 18 publications

(18 citation statements)

References 53 publications

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“…Therefore, the angular deviation between the AO and the BBO could be related in an adaptive manner, as the AO might result from hydrodynamic interactions, and thus, represent an indicator of the prevailing rheology. In a similar morphofunctional study performed on the ciliated epithelium of the bovine oviduct Schätz et al (2013), we have recently shown that the AO is exactly aligned along the longitudinal axis of the fallopian tube. Furthermore, compared to the mucociliary epithelial surface of the airways, altered rheological conditions are found in the fallopian tube.…”

Section: Discussionmentioning

confidence: 70%

“…to the mucociliary transport direction and to the wave propagation direction, which were previously determined on bovine trachea explants by using reflection contrast microscopy Burn (2009). The microscopy setup as well as the applied image processing methods were previously described in Ryser et al (2007) and subsequently employed in Schätz et al (2013); Wyss et al (2018). Relating the structural orientations AO and BBO to the three characteristic functional directions: 1) the sampling-corrected circular mean mucociliary transport direction of 16.1° ± 5.4°, 2) the sampling-corrected circular mean direction of the mean of harmonic plane waves propagating into the laryngeal direction, which was found to be 16.7° ± 6.3°, and 3) the sampling-corrected circular mean propagation direction of the mean wave of 11.7° ± 6.8° it becomes evident that only the AO indicates the effective stroke direction, which is in line with the mucociliary transport and the direction of the harmonic mean plane wave propagation into the laryngeal direction.…”

Section: Discussionmentioning

confidence: 99%

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Spatial Intra- and Intercellular Alignment of Respiratory Cilia and its Relation to Function

Schneiter

Halm

Odriozola

et al. 2019

Preprint

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Ciliary alignment is considered necessary to establish respiratory tract 4 mucociliary clearance, and disorientation is o en associated with primary 5 ciliary dyskinesia. erefore, there is an urgent need for a detailed analy-6 sis of ciliary orientation (CO). We used volume electron microscopy to ex-7 amine CO relative to the tracheal long axis (TLA) by measuring the inter-8 and intracellular basal body orientation (BBO) and axonemal orientation 9 (AO), which are considered to coincide, both equivalently indicating the 10 e ective stroke direction. Our results, however, reveal that only the mean 11 BBO is aligned with the TLA, whereas the AO determines the e ective 12 stroke direction as well as the mucociliary transport direction. Further-13 more, we show that even if the mean CO is conserved across cell bound-14 aries, a considerable gradient in CO exists within individual cells, which 15 we suspect to be crucial for the emergence of coordinated ciliary activity. 16 1 Our ndings provide new quantitative insight into CO and correlate this 17 new structural information with mucociliary function. 18 Introduction 19 e epithelium of the tracheobronchial tree constitutes a self-cleaning surface, which mainly 20 consists of secretory goblet cells and ciliated cells. Dust and infectious particles enter the 21 respiratory tract when breathing. Due to adhesion, these particles get entrapped by the mucus 22 layer lining the inner surface of the airways. Coordinated movements of billions of subjacent 23 cilia then propel the overlying mucous carpet towards the larynx, thereby cleaning the lungs 24 from inhaled substances. 25 Cilia are hair-like protrusions of the cell membrane with a particular cytoskeletal sca old 26 called axoneme. e ciliary axoneme consists of an evolutionary preserved 9+2 microtubular 27 structure: a central pair is surrounded by nine circularly aligned microtubule doublets. e 28 microtubules arise from the basal bodies, which are anchored to the cytoskeleton and serve as 29 nucleation sites for the growth of axonemal microtubules. e chiral structure of the axoneme 30 as well as of the basal foot appendage allows to unequivocally determine ciliary orientation 31 (CO). 32 e physical orientational alignment of motile cilia along the proximal-distal axis of the 33 airways is a prerequisite for concerted directional ciliary movement and the generation of 34 directed uid ow (e.g. Vladar et al. (2015); Guirao and Joanny (2007)). Studies a empting to 35 quantify the orientation of respiratory cilia usually determined the direction of the assumed 36 ciliary beating plane, or more speci cally, the direction of the 'e ective stroke' (or 'power 37 stroke'). According to the literature, the e ective stroke direction is unambiguously de ned, 38 and can be inferred from two chiral ciliary structures: 1) the ciliary beating plane is assumed 39 to be perpendicular to the central pair of microtubules, and in particular, the e ective stroke 40 is assumed to be directed from doublet 1 towards the gap between doublet 5...

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Spatial Intra- and Intercellular Alignment of Respiratory Cilia and its Relation to Function

Schneiter

Halm

Odriozola

et al. 2019

Preprint

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show abstract

“…Sialic acid residues could keep the cilia separated from one another, maintain ciliary motility [87] and provide the progression of capacitated spermatozoa [88]. In addition, the cilia sialoglycoproteins could regulate the mucociliar clearance because the ciliary beating ensures mucociliar clearance of the oviduct [11].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Ciliated cells play a role in the ovum transport and the mucociliary propagation towards the uterus [11]. roles in fertilization and/or early embryonic development because they could associate with ovulated ova, developing embryos and spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Modifications of carbohydrate residues in the sheep oviductal ampulla after superovulation

et al. 2015

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“…CCs play a role in the ovum transport and in the mucociliary propagation toward the uterus [27]. NCs are secretory cells and their activity determines, together with a selective transudate of serum, the composition of the fluid in the oviduct.…”

Section: Introductionmentioning

confidence: 99%