Cimicifuga racemosa and its triterpene-saponins prevent the Metabolic Syndrome and deterioration of cartilage in the knee joint of ovariectomized rats by similar mechanisms

Seidlová-Wuttke, Dana; Eder, Nicole; Stahnke, Vera; Kammann, Markus; Stecher, Günter; Haunschild, Jutta; Wessels, Johannes T.; Wuttke, W.

doi:10.1016/j.phymed.2012.03.001

Cited by 17 publications

(8 citation statements)

References 30 publications

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“…Consistently, our results showed hyperglycemia and signi cant elevation in all lipid pro le parameters such as cholesterol, triglyceride, LDL, and VLDL with a declined HDL level in LTZ induced PCOS rats compared with negative control (Table 1), in agreement with other published reports (76)(77)(78). Hyperglycemic and dyslipidemic effects observed in black cohosh rats were related to PCOS, while AR did not affect the lipid pro le and glucose in women (79,80). However, vitamin C exerted an obvious enhancement in glucose and lipid metabolism, and increased the HDL levels, which were previously detected in both diabetic patients (81) and rats (82).…”

Section: Discussionsupporting

confidence: 92%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Azouz

Ali

Abd‐Elsalam

et al. 2020

Preprint

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Background: Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for treatment of polycystic ovary syndrome (PCOS). However, the AR hepatotoxicity urges a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products.Methods: We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed then rats received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, a holistic profiling of AR arsenal of secondary metabolites was performed using ultra high-performance liquid chromatography (UHPLC) coupled with quadrupole high resolution time of flight mass spectrometry (QTOF-MS).Results: Beneficial effects were exerted by AR in PCOS rats via the improved antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic MDA and GSH levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes.Conclusion: This study demonstrated the benefit of the combination between AR and vitamin C in mitigating the reproductive and metabolic disorders of PCOS with the elimination of AR hepatotoxic risk.

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Section: Discussionsupporting

confidence: 92%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Azouz

Ali

Abd‐Elsalam

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Consistently, our results showed hyperglycemia and significant elevation in all lipid profile parameters such as cholesterol, triglyceride, LDL, and VLDL with a declined HDL level in LTZ induced PCOS rats compared with negative control (Table 1), in agreement with other published reports [77][78][79]. Hyperglycemic and dyslipidemic effects observed in black cohosh rats were related to PCOS, while AR did not affect the lipid profile and glucose in women [80,81]. However, vitamin C exerted an obvious enhancement in glucose and lipid metabolism, and increased the HDL levels, which were previously detected in both diabetic patients [82] and rats [83].…”

Section: Discussionsupporting

confidence: 92%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect

et al. 2021

View full text Add to dashboard Cite

Background Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for the treatment of polycystic ovary syndrome (PCOS). However, AR-induced hepatotoxicity necessitates a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products. Methods We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed. The rats then received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, holistic profiling of the AR arsenal of secondary metabolites was performed using ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (QTOF-MS). Results Beneficial effects were exerted by AR in PCOS rats as antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells were all improved. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic malondialdehyde (MDA) and glutathione (GSH) levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes. Conclusion This study demonstrated the benefit of the combinatorial effects of AR and vitamin C in mitigating the reproductive and metabolic disorders associated with PCOS with the elimination of AR hepatotoxic risk.

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“…Consistently, our results showed hyperglycemia and significant elevation in all lipid profile parameters; cholesterol, triglyceride, LDL, VLDL and reduction in HDL in LTZ induced PCOS rats compared with normal control (Table 1), (65)(66)(67). Hyperglycemic and dyslipidemic effects observed in black cohosh rats were related to PCOS, while AR does not affect lipid profile and glucose in women (68,69). Vitamin C exerted obvious enhancement in glucose and lipid metabolism, and increased the HDL level, which were previously detected in both diabetic patients (70) and rats (71).…”

Section: Discussionsupporting

confidence: 86%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in Letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Azouz

Ali

Abd‐Elsalam

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemose ‘AR’) are being sought to surmount the shortcomings of conventional hormonal and surgical therapies developed in the treatment of polycystic ovary syndrome (PCOS). However, the AR hepatotoxicity urges a cautionary warning to be labelled on its products as recommended by the United States Pharmacopeia where 4 out of 7 hepatotoxic cases in Sweden were possibly associated with black cohosh products.Methods We investigated the efficacy, safety and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality, oxidative response in the hyperandrogenism-induced PCOS alongside with holistic profiling of its arsenal of secondary metabolites using UPLC-Qtof-MS. A well-established rat model using oral letrozole, daily, for 21 days was employed then the rats received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, apoptosis, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated.Results Beneficial effects were exerted by AR in PCOS rats via the improved antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, curtailed percentage of apoptotic cells and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells. Notably, AR combination with vitamin C was not only more effective to reverse the dysregulated levels of testosterone, luteinising hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer, while the combination regulated both ovarian and hepatic MDA and GSH levels with a histologic improvement noticed in livers and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes.Conclusion This study demonstrated the benefit of the combination between AR and vitamin C in mitigating the reproductive and metabolic disorders of PCOS with the elimination of AR hepatotoxic risk.

show abstract

Cimicifuga racemosa and its triterpene-saponins prevent the Metabolic Syndrome and deterioration of cartilage in the knee joint of ovariectomized rats by similar mechanisms

Cited by 17 publications

References 30 publications

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in Letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

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