CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

Ragel, Paula; Ródenas, Reyes; García‐Martín, Elena; Andrés, Zaida; Villalta, Irène; Nieves‐Cordones, Manuel; Rivero, Rosa M.; Martı́nez, Vicente; Pardo, José M.; Quintero, Francisco J.; Rubio, Francisco

doi:10.1104/pp.15.01401

Cited by 168 publications

(250 citation statements)

References 63 publications

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“…However, only the overexpression of CIPK23 enhanced the Arabidopsis K + uptake capacity and LK tolerance (Xu et al, 2006). Combined with the recent finding that CIPK23 also activates the HAK5 transporter by phosphorylation (Ragel et al, 2015), the LK-tolerant phenotype of CIPK23-overexpressing lines may be due to enhanced K + uptake from both AKT1 and HAK5. In contrast, the AtKC1-overexpressing lines exhibit a similar shoot phenotype to that of wild-type plants on LK medium (Wang et al, 2010).…”

Section: The Relationship Between Atkc1 and Cipk23 In Akt1 Regulationmentioning

confidence: 90%

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AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

et al. 2016

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In Arabidopsis (Arabidopsis thaliana), the Shaker K + channel AKT1 conducts K + uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K + (LK) response; however, the relationship between them remains unclear. In this study, we screened suppressors of low-K + sensitive [lks1 (cipk23)] and isolated the suppressor of lks1 (sls1) mutant, which suppressed the leaf chlorosis phenotype of lks1 under LK conditions. Map-based cloning revealed a point mutation in AtKC1 of sls1 that led to an amino acid substitution (G322D) in the S6 region of AtKC1. The G322D substitution generated a gain-of-function mutation, AtKC1 D , that enhanced K + uptake capacity and LK tolerance in Arabidopsis. Structural prediction suggested that glycine-322 is highly conserved in K + channels and may function as the gating hinge of plant Shaker K + channels. Electrophysiological analyses revealed that, compared with wild-type AtKC1, AtKC1 D showed enhanced inhibition of AKT1 activity and strongly reduced K + leakage through AKT1 under LK conditions. In addition, phenotype analysis revealed distinct phenotypes of lks1 and atkc1 mutants in different LK assays, but the lks1 atkc1 double mutant always showed a LK-sensitive phenotype similar to that of akt1. This study revealed a link between CIPK-mediated activation and AtKC1-mediated modification in AKT1 regulation. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K + uptake/leakage to modulate AKT1-mediated LK responses in Arabidopsis.

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Section: The Relationship Between Atkc1 and Cipk23 In Akt1 Regulationmentioning

confidence: 90%

“…The transcription of HAK5 is induced by K + deficiency (Gierth et al, 2005), which is an important mechanism in plant responses to LK stress . Recent reports showed that HAK5 and its ortholog in Dionaea muscipula (DmHAK5) are also regulated by the protein kinase CIPK23 (Ragel et al, 2015;Scherzer et al, 2015).…”

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confidence: 99%

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

et al. 2016

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“…9D), where HAK5 is the only transport system contributing to K + uptake (Rubio and Rodríguez-Navarro, 2000). Although HAK5 phosphorylation has not yet been shown in planta, several studies strongly indicate that HAK5 activity status is modulated by phosphorylation of its N terminus (Jones et al, 2009;Rubio et al, 2014;Ragel et al, 2015;Scherzer et al, 2015). Co-expression of the CBLs and CIPK23 also increases HAK5 transport through CIPK23 activity, indicating that HAK5 conformation may be regulated by phosphorylation during K + deficiency (Ragel et al, 2015;Scherzer et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In Dionaea muscipula, DmHAK5 mediated K + uptake in Xenopus oocytes but only when co-expressed with the Ca 2+ -induced AtCBL9-CIPK23 complex (Scherzer et al, 2015), suggesting that the transport activity is regulated by Ca 2+ signals. CBL1 and CIPK23 were also found to enhance AtHAK5 transport in yeast and Arabidopsis (Ragel et al, 2015). Thus, K + deficiency promotes HAK5 regulation at multiple levels and modifies K + uptake according to plant needs.…”

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confidence: 97%

The Raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for Innate Immunity and Abiotic Stress Response

et al. 2016

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Plant perception of pathogen-associated molecular patterns (PAMPs) and other environmental stresses trigger transient ion fluxes at the plasma membrane. Apart from the role of Ca 2+ uptake in signaling, the regulation and significance of PAMPinduced ion fluxes in immunity remain unknown. We characterized the functions of INTEGRIN-LINKED KINASE1 (ILK1) that encodes a Raf-like MAP2K kinase with functions insufficiently understood in plants. Analysis of ILK1 mutants impaired in the expression or kinase activity revealed that ILK1 contributes to plant defense to bacterial pathogens, osmotic stress sensitivity, and cellular responses and total ion accumulation in the plant upon treatment with a bacterial-derived PAMP, flg22. The calmodulin-like protein CML9, a negative modulator of flg22-triggered immunity, interacted with, and suppressed ILK1 kinase activity. ILK1 interacted with and promoted the accumulation of HAK5, a putative (H + )/K + symporter that mediates a high-affinity uptake during K + deficiency. ILK1 or HAK5 expression was required for several flg22 responses including gene induction, growth arrest, and plasma membrane depolarization. Furthermore, flg22 treatment induced a rapid K + efflux at both the plant and cellular levels in wild type, while mutants with impaired ILK1 or HAK5 expression exhibited a comparatively increased K + loss. Taken together, our results position ILK1 as a link between plant defense pathways and K + homeostasis.

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“…Крім того, вивчався біохімічний метаболізм даного ензиму та визначено негативний вплив пероксидази на ріст та розвиток рослин класу Arabidopsis в умовах пошкодження клітинної стінки (Raggi et al, 2015). Визначена роль пероксидази і у мембранному транспорті та біоенергетиці при формуванні активних форм кисню клітинами кореневої системи в умовах нестачі калію (Ragel, 2015). Виявлені фізіологічні механізми внеску пероксидази у термін зберігання томатів .…”

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Вплив Регулятору Росту На Ферменти Антиоксидантного Захисту За Умов Діі Антропогенних Емісій

Vasilyuk

2016

Biol. Bull. of Bogdan Chmelnitskiy Melitopol St. Pedag. Univ.

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Досліджено загальну (GА, Px, ∆Е, мл•с) та питому (SА, Px, ∆Е, мг•с) активність ферменту класу оксидоредуктаз пероксидази (Px, EC 1.11.1.7), яка у поєднанні із каталазою (Cat, EC 1.11.1.6) формує подвійну ланку антиоксидантного захисту клітин в організмах різного рівня організації за умов підвищення окисно-відновних процесів при збільшенні екзогенного навантаження, як неспецифічної реакція на стрес. Дані показники, як маркери зміни чинників довкілля, визначали в листках Salix alba L., що розповсюджена по берегам річки Мокра Сура (антропогенно забруднена з підвищеною мінералізацією) та річки Шпакова (умовно чиста, контроль). Дані акваторії належать до басейну річки Дніпро Степового Придніпров'я, що характеризуються значним індустріальним навантаженням. Для пришвидшення вкорінення та зниження екзогенного антропогенного тиску на рослинний об'єкт використовували регулятор росту рослин «Корневін». Виявлено неспецифічну реакцію пероксидази на антропогенний тиск, визначено достовірні відмінності між дослідом та контролем у показниках антиоксидантного захисту залежно від умов росту та розвитку. Ключові слова: Salix alba L., підвищена мінералізація, регулятори росту, пероксидаза, адаптогени, антропогенні чинники ЕFFECT OF PLANT GROWTH REGULATORS ON ANTIOXIDANT ENZYMES PROTECTION UNDER ANTHROPOGENIC EMISSIONS O.M. Vasilyuk Oles' Gonchar Dnipropetrovs'k National University E-mail: Vasilyuk.elena@mail.ruThe paper presents analysis of general (GА, Px, ∆Е, ml•sec) and specific (SA, Px, ∆Е, mg•sec) of oxidoreductase enzyme activity (Peroxidase, Px, EC 1.11.1.7), which together with Catalase (Cat, EC 1.11.1.6) forms a double link antioxidant protection of cells in the organisms of different levels of organization (non-specific response to stress) in the conditions of increasing redox processes under the exogenous stress. These markers of changeable environmental factors were sampled in the leaves of Salix alba L., which grew along Mokra Sura River (anthropogenically polluted with high level of salinity, experiment) and Shpakova River (without anthropogenic impact, control). These rivers belong to the basin of the Dnieper River (Steppe Dnieper region) that has high industrial load. We used plant growth regulator "Kornevin" in order to accelerate the rooting and reduce the exogenous pressures on the plants. We detected nonspecific reaction towards peroxidase in anthropogenic pressure conditions and determined significant differences between experiment and control regards antioxidant protection depending on growth and development conditions.

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CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

Cited by 168 publications

References 63 publications

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

The Raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for Innate Immunity and Abiotic Stress Response

Вплив Регулятору Росту На Ферменти Антиоксидантного Захисту За Умов Діі Антропогенних Емісій

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