Circ_0109046 promotes the malignancy of endometrial carcinoma cells through the <scp>microRNA</scp>‐105/<scp>SOX9</scp>/Wnt/β‐catenin axis

Li, Yanyan; Liu, Jinyu; Piao, Jinxia; Jiang, Ou; Zhu, Xiaoyan

doi:10.1002/iub.2415

Cited by 19 publications

(13 citation statements)

References 50 publications

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“…A vast array of abnormally expressed circRNAs, such as circ_0005276 [ 2 ], circ_0004390 [ 3 ], and circPIP5K1A [ 4 ], have been discovered in ovarian cancer, which are closely related to the clinicopathological features and prognosis of ovarian cancer patients; moreover, functional experiments further confirmed their involvement in the tumorigenesis of ovarian cancer, implying that circRNAs may be promising candidates for the development of ovarian cancer therapeutic method. Recently, research showed that circ_0109046 was highly expressed in endometrial cancer and predicted poor prognosis; functionally, circ_0109046 lack inhibited the proliferation and invasiveness of endometrial cancer cells by miR-105 [ 5 ]. However, the action and mechanism of circ_0109046 on the ovarian cancer cell malignant phenotypes remain vague.…”

Section: Introductionmentioning

confidence: 99%

Effects of Circ_0109046 Regulating Mir-338-3p on the Malignant Behavior of A2780 Cells

Zhao

Diao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The objective is to explore the action and mechanism of circ_0109046 on the malignant phenotypes of ovarian cancer cells. Methods. Circ_0109046 and miR-338-3p expression were detected by quantitative real-time polymerase chain reaction (qRT-PCR). In vitro assays were conducted to investigate the action of circ_0109046 and miR-338-3p on ovarian cancer cell growth and metastasis. Western blotting was utilized to investigate the contents of apoptosis-related markers. The binding between circ_0109046 and miR-338-3p was validated using dual-luciferase reporter assay. Results. Circ_0109046 was increased, while miR-338-3p content was decreased in ovarian cancer tissues. Deficiency of circ_0109046 or the upregulation of miR-338-3p was observed to weaken cell proliferative, migratory, and invasive abilities and elevated cell apoptosis rate in ovarian cancer. Circ_0109046 targetedly suppressed miR-338-3p. Down-regulation of miR-338-3p was able to reverse the repressing impacts of circ_0109046 silencing on ovarian cancer growth and mobility. Conclusion. Circ_0109046 silencing impaired the proliferation, migration, and invasion of ovarian cancer cells through negatively regulating miR-338-3p in vitro, indicating the potential implication of circ_0109046 in ovarian cancer progression.

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Section: Introductionmentioning

confidence: 99%

Effects of Circ_0109046 Regulating Mir-338-3p on the Malignant Behavior of A2780 Cells

Zhao

Diao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…25 Circ_0109046 absence hindered cell growth and epithelial-mesenchymal transition in EC. 26 In another example, Li and his colleague 27 indicated circ_0109046 depletion impeded cell aggressiveness and promoted cell apoptosis in EC in vitro. CircZNF124, a circRNA, has been reported to inhibit lung cancer processes by miR-498/YES proto-oncogene 1 axis.…”

Section: Discussionmentioning

confidence: 99%

CircZNF124 regulates cell proliferation, leucine uptake, migration and invasion by miR‐199b‐5p/SLC7A5 pathway in endometrial cancer

Shu

Peng

Zhong

et al. 2021

Immunity Inflam & Disease

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Background: Recent studies have revealed that circular RNA participates in endometrial carcinoma (EC) progression. Here we investigated the role of circRNA zinc finger protein 124 (circZNF124) in EC genesis and underlying mechanism. Methods:The expression levels of circZNF124, and solute carrier family 7 member 5 (SLC7A5) were detected by quantitative real-time polymerase chain reaction. The expression of SLC7A5 and other indicated marker proteins was determined by western blot analysis. For functional assay, cell proliferation, leucine uptake and metastasis were investigated by total cell number, cell counting kit-8, cell colony formation, leucine uptake or transwell assay. The interaction between miR-199b-5p and circZNF124 or SLC7A5 was predicted by starbase online database, and identified by mechanism assays. The impact of circZNF124 absence on tumor growth in vivo was revealed by xenograft mouse model assay. Immunohistochemistry assay was implemented to detect the positive expression rate of nuclear proliferation marker (Ki67).Results: CircZNF124 and SLC7A5 expression were significantly increased, while miR-199b-5p was decreased in EC tissues and cells compared with normal endometrial tissues or cells. CircZNF124 expression was closely associated with EC severity and lymph node metastasis. Additionally, circZNF124 depletion repressed cell proliferation, leucine uptake, migration and invasion in both HEC1A and Ishikawa cells. CircZNF124 regulated SLC7A5 expression by binding to miR-199b-5p. MiR-199b-5p inhibitors or SLC7A5 overexpression attenuated circZNF124 silencing-mediated EC malignant progression. Furthermore, SLC7A5 absence inhibited tumor growth in vivo.

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“…In the studies of ovarian cancer, HK2, which is regulated by the tumor microenvironment, controls the production of lactic acid and regulates the expression of MMP9/NAONG/SOX9 through the FAK/ERK1/2 signaling pathway, which leads to ovarian cancer metastasis and stem regulation [ [70]. SOX9 expression also showed a signi cant gradual increase from normal to grade 1 to grade 2/3 tumors [71].…”

Section: Discussionmentioning

confidence: 99%

Identification of Hub Gene in Cervical Cancer by Weighted Gene Co-Expression Network Analysis

Chen¹,

Huang²,

Zhang³

et al. 2021

Preprint

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Background:Cervical cancer(CC) is one of the most common malignant tumors in gynecology. Both its incidence and mortality are high. Despite advances in screening, diagnosis, prevention, and treatment, CC is still one of the leading causes of cancer-related death in women. However, in the pathogenesis of CC, the exact molecular mechanism is still unclear. It may be a multi-gene, multi-factor, multi-step, and multi-stage complex process. Hence, the pathogenesis and molecular mechanism of CC are the keys to effective treatment of it. In this study, we tried to identify candidate biomarkers for cervical cancer through weighted gene co-expression network analysis (WGCNA). Methods: The gene expression profile of CESC was downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed genes (DEGs) were analyzed by the Limma package, and the gene co-expression module was constructed by WGCNA. Use the online website STRING to construct the protein interaction network of genes in significant modules, and then use Cytoscape analysis to find the 10 most important node degree genes. Results: Among these 10 genes, SOX9 expression was associated with the prognosis of cervical cancer patients. Immunohistochemical results from the online website human protein atlas showed that SOX9 protein expression was significantly higher in cervical cancer tissues than in normal cervical tissues. After collecting cancerous and paracancerous tissue specimens from 16 patients with cervical cancer, Q-PCR showed that the mRNA expression levels of SOX9 in cervical cancer tissues were significantly greater than those in normal adjacent tissues. Conclusions: The elevated expression of SOX9 is significantly related to the disease-free survival and overall survival of cervical cancer. Therefore, the SOX9 gene could be used as an indicator of cervical cancer diagnosis and prognosis.

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Circ_0109046 promotes the malignancy of endometrial carcinoma cells through the microRNA‐105/SOX9/Wnt/β‐catenin axis

Cited by 19 publications

References 50 publications

Effects of Circ_0109046 Regulating Mir-338-3p on the Malignant Behavior of A2780 Cells

Effects of Circ_0109046 Regulating Mir-338-3p on the Malignant Behavior of A2780 Cells

CircZNF124 regulates cell proliferation, leucine uptake, migration and invasion by miR‐199b‐5p/SLC7A5 pathway in endometrial cancer

Identification of Hub Gene in Cervical Cancer by Weighted Gene Co-Expression Network Analysis

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