Circ_0123996 promotes cell proliferation and fibrosis in mouse mesangial cells through sponging miR-149-5p and inducing Bach1 expression

Wang, Wenjin; Feng, Jingfang; Zhou, Hongying; Li, Qi

doi:10.1016/j.gene.2020.144971

Cited by 34 publications

(18 citation statements)

References 27 publications

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“…In human umbilical vein endothelial cells (HUVECs), miR-149-5p expression reduced after high glucose treatment [ 30 ]. Expression of miR-149-5p reduced in diabetic nephropathy, which promotes mesangial cell proliferation and fibrosis in mice [ 31 ]. miR-149-5p expression also reduced in high glucose-treated MIN6 cells, and the miR-149-5p mimic protected cell survival and insulin secretion while reduced cell apoptosis by inhibiting the expression of Bcl2-like 11 [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

Astragalus polysaccharide improve the proliferation and insulin secretion of mouse pancreatic β cells induced by high glucose and palmitic acid partially through promoting miR-136-5p and miR-149-5p expression

Deng

Yang

et al. 2021

Bioengineered

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The current drugs for the treatment of type 2 diabetes mellitus (T2DM) can cause side effects after long-time use. Hence, the novel drugs were urgent need to developed for T2DM patients. In this study, the effect of astragalus polysaccharide on dysfunctional insulin cells was investigated to clarify whether astragalus polysaccharide could be a novel drug for T2DM treatment. MIN6 cells (mouse pancreatic β-cell line) were treated with high glucose (HG)+ palmitic acid (PA) and then treated with astragalus polysaccharide. The proliferation, apoptosis, and insulin secretion were measured using CCK8, flow cytometry, and ELISA, respectively. Pancreatic and duodenal homeobox 1 (PDX1), miR-136-5p, and miR-149-5p expression levels were measured by RT-qPCR. The combination of EF-hand domain family member 2 (EFHD2) and miR-136-5p or miR-149-5p was analyzed by luciferase reporter assay. EFHD2 protein level was measured by western blot. We found that HG+PA treatment reduced MIN6 cell viability, insulin secretion, and PDX1 expression and promoted MIN6 cell apoptosis. Astragalus polysaccharide treatment reversed the effect of HG+PA on MIN6 cells. Additionally, astragalus polysaccharide treatment promoted miR-136-5p and miR-149-5p expression. Silencing of miR-136-5p and miR-149-5p expression partially reversed the therapeutic effects of astragalus polysaccharide. Furthermore, EFHD2 was the target of miR-136-5p and miR-149-5p. Meanwhile, astragalus polysaccharide treatment inhibited EFHD2 protein level in HG+PA treated MIN6 cell. Finally, EFHD2 overexpression partially reversed the therapeutic effects of astragalus polysaccharide. In conclusion, astragalus polysaccharide treatment improved proliferation and insulin secretion in HG+PA-treated MIN6 cells partially by promoting miR-136-5p and miR-149-5p expression to inhibit EFHD2 expression.

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Section: Resultsmentioning

confidence: 99%

Astragalus polysaccharide improve the proliferation and insulin secretion of mouse pancreatic β cells induced by high glucose and palmitic acid partially through promoting miR-136-5p and miR-149-5p expression

Deng

Yang

et al. 2021

Bioengineered

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“…Finally, even though their exact mechanistic role remains relatively elusive, sscRNAs have been associated with contribution to fibrosis [124]. In diabetic nephropathy, the knockdown of various sscRNAs, including circ_0123996 [125], circ_0000064 [126] and circ_WBSCR17 [127], in mesangial and tubular cells by siRNA or miRNA mimetics resulted in reduction of fibronectin, collagen, and TGF-β1. Mechanistic exploration of the role of these sscRNAs revealed that their pro-fibrotic actions rely on the sponging of endogenous regulator miRNAs that inhibit the production of fibrotic proteins.…”

Section: Mirna Lncrna and Sscrna Nucleotides As Anti-fibrotic Targetsmentioning

confidence: 99%

Therapeutic and diagnostic targeting of fibrosis in metabolic, proliferative and viral disorders

Sofias

Lorenzi

Peña

et al. 2021

Advanced Drug Delivery Reviews

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“…Circ_0000491 aggravated ECM and fibrosis-associated protein synthesis through suppressing miR-101b which targets TGFβRI ( 68 ). Circ_0123996 was able to promote MCs proliferation and fibrosis through functioning as the sponge for miR-149-5p and inducing Bach1 expression ( 92 ). Circ_00037128/miR-17-3p/AKT3 axis also facilitated DKD progression via modulating MCs proliferation and fibrosis ( 93 ).…”

Section: Circrnas As Therapeutic Targets For Kidney Diseasesmentioning

confidence: 99%

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

Xie²,

Huang

et al. 2021

Front. Med.

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Circular RNAs (circRNAs) are a novel type of non-coding RNAs that have aroused growing attention in this decade. They are widely expressed in eukaryotes and generally have high stability owing to their special closed-loop structure. Many circRNAs are abundant, evolutionarily conserved, and exhibit cell-type-specific and tissue-specific expression patterns. Mounting evidence suggests that circRNAs have regulatory potency for gene expression by acting as microRNA sponges, interacting with proteins, regulating transcription, or directly undergoing translation. Dysregulated expression of circRNAs were found in many pathological conditions and contribute to the pathogenesis and progression of various disorders, including renal diseases. Recent studies have revealed that circRNAs may serve as novel reliable biomarkers for the diagnosis and prognosis prediction of multiple kidney diseases, such as renal cell carcinoma (RCC), acute kidney injury (AKI), diabetic kidney disease (DKD), and other glomerular diseases. Furthermore, circRNAs expressed by intrinsic kidney cells are shown to play a substantial role in kidney injury, mostly reported in DKD and RCC. Herein, we review the biogenesis and biological functions of circRNAs, and summarize their roles as promising biomarkers and therapeutic targets in common kidney diseases.

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Circ_0123996 promotes cell proliferation and fibrosis in mouse mesangial cells through sponging miR-149-5p and inducing Bach1 expression

Cited by 34 publications

References 27 publications

Astragalus polysaccharide improve the proliferation and insulin secretion of mouse pancreatic β cells induced by high glucose and palmitic acid partially through promoting miR-136-5p and miR-149-5p expression

Astragalus polysaccharide improve the proliferation and insulin secretion of mouse pancreatic β cells induced by high glucose and palmitic acid partially through promoting miR-136-5p and miR-149-5p expression

Therapeutic and diagnostic targeting of fibrosis in metabolic, proliferative and viral disorders

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

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