Circadian Control of Membrane Excitability in Drosophila melanogaster Lateral Ventral Clock Neurons

Cao, Guojie; Nitabach, Michael N.

doi:10.1523/jneurosci.1503-08.2008

Cited by 147 publications

(214 citation statements)

References 41 publications

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“…Interestingly, the firing rate of PDF-containing neurons, the l-LNvs as well as the s-LNvs, is also maximal near the beginning of the day (28,33), in DD as well as LD; this is also the likely time of maximal PDF release from s-LNv dorsal projections (34). In addition, the l-LNvs promote light-mediated arousal, also mediated at least in part by PDF (23,29,35).…”

Section: Discussionmentioning

confidence: 95%

PDF and cAMP enhance PER stability in Drosophila clock neurons

Guo

Shen

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance This work describes an advance in the understanding of how the important circadian neuropeptide PDF contributes to the function of the molecular clock in Drosophila neurons. The famous per S allele of period significantly improves the rhythmicity of flies missing PDF. The per S gene product degrades rapidly, suggesting that PDF-mediated cAMP affects PER stability. Indeed, increasing cAMP levels and cAMP-mediated protein kinase A (PKA) activity stabilizes PER, in tissue culture cells and in circadian neurons. PDF addition to fly brains in vitro has a similar effect. Our observations taken together indicate that PDF contributes to clock neuron function by activating PKA, stabilizing PER, and thereby slowing the pace of clock neurons that respond to PDF.

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Section: Discussionmentioning

confidence: 95%

PDF and cAMP enhance PER stability in Drosophila clock neurons

Guo

Shen

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…l-LNv recordings were performed on acutely dissected adult fly brains in whole-cell current clamp mode (1,2). Extended information on materials and methods is described in SI Materials and Methods, including protocols for electrophysiology, optics, genetics, behavioral testing, and statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Blue-lightactivated CRY couples to membrane depolarization in Drosophila LNv neurons by a redox-based mechanism involving potassium channel heteromultimeric complexes, consisting of the downstream redox sensor cytoplasmic potassium beta (Kvβ), HYPERKINETIC (HK), and ion-conducting voltage-gated potassium alpha (Kvα) ether-a-go-go family subunits (8). Electrical activity in the LNvs contributes to circadian rhythms (9)(10)(11), and, reciprocally, LNv neuronal firing rate is circadian-regulated (1,2,12). Circadian regulation of firing rate is widely conserved in other invertebrate and vertebrate species (13,14).…”

mentioning

confidence: 99%

CRYPTOCHROME mediates behavioral executive choice in response to UV light

Baik

Fogle

Roberts

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Drosophila melanogaster CRYPTOCHROME (CRY) mediates behavioral and electrophysiological responses to blue light coded by circadian and arousal neurons. However, spectroscopic and biochemical assays of heterologously expressed CRY suggest that CRY may mediate functional responses to UV-A (ultraviolet A) light as well. To determine the relative contributions of distinct phototransduction systems, we tested mutants lacking CRY and mutants with disrupted opsin-based phototransduction for behavioral and electrophysiological responses to UV light. CRY and opsin-based external photoreceptor systems cooperate for UV light-evoked acute responses. CRY mediates behavioral avoidance responses related to executive choice, consistent with its expression in central brain neurons.cryptochrome | phototransduction | UV | neural decision making | Drosophila

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“…This is well documented for clock cells in the mammalian brain clock, the suprachiasmatic nucleus, and has also been demonstrated for some of the Drosophila clock neurons. Although the all-important sLN v s have been difficult to access for electrophysiology, recordings from large LN v s and DN1 neurons show increased action potential firing rates in the early morning that decrease through the day and then begin to rise again during the night (Sheeba et al 2007(Sheeba et al , 2008aCao and Nitabach 2008;Flourakis and Allada 2016). Consistent with their peptidergic identity, LN v s also show rhythms of firing morphology, with higher burst firing during the day and tonic firing or no firing at night (Sheeba et al 2007(Sheeba et al , 2008aCao and Nitabach 2008).…”

mentioning

confidence: 94%

Circadian and feeding cues integrate to drive rhythms of physiology in Drosophila insulin-producing cells

et al. 2016

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Circadian clocks regulate much of behavior and physiology, but the mechanisms by which they do so remain poorly understood. While cyclic gene expression is thought to underlie metabolic rhythms, little is known about cycles in cellular physiology. We found that Drosophila insulin-producing cells (IPCs), which are located in the pars intercerebralis and lack an autonomous circadian clock, are functionally connected to the central circadian clock circuit via DN1 neurons. Insulin mediates circadian output by regulating the rhythmic expression of a metabolic gene (sxe2) in the fat body. Patch clamp electrophysiology reveals that IPCs display circadian clock-regulated daily rhythms in firing event frequency and bursting proportion under light:dark conditions. The activity of IPCs and the rhythmic expression of sxe2 are additionally regulated by feeding, as demonstrated by night feeding-induced changes in IPC firing characteristics and sxe2 levels in the fat body. These findings indicate circuit-level regulation of metabolism by clock cells in Drosophila and support a role for the pars intercerebralis in integrating circadian control of behavior and physiology.

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Circadian Control of Membrane Excitability in Drosophila melanogaster Lateral Ventral Clock Neurons

Cited by 147 publications

References 41 publications

PDF and cAMP enhance PER stability in Drosophila clock neurons

PDF and cAMP enhance PER stability in Drosophila clock neurons

CRYPTOCHROME mediates behavioral executive choice in response to UV light

Circadian and feeding cues integrate to drive rhythms of physiology in Drosophila insulin-producing cells

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