Circadian oscillation of innate immunity components in mouse small intestine

Froy, Oren; Chapnik, Nava

doi:10.1016/j.molimm.2006.09.026

Cited by 92 publications

(75 citation statements)

References 37 publications

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“…Our data show that clock genes are expressed and exhibit circadian oscillations in the small intestine showing peak expressions at different times of the day and are consistent with their expression in enterocytes and Paneth cells ( 13 ). However, we noticed for the fi rst time that circadian expression of the clock genes was dampened or lost in Clk mt/mt mice.…”

Section: Light-and Food-entrained Regulation Of Clock Genes In the Gusupporting

confidence: 83%

“…Thus, this study has uncovered a role for circadian rhythm and Clock protein in macronutrient absorption by the intestine. [4][5][6][7][8][9][10][11][12][13][14] C]cholesterol (58.0 mCi/mmol), [9, H(N)]triolein (22.0 Ci/mmol), [9,10-µm thick), mounted on glass slides, fi xed in ethanol for 15 min at Ϫ 15°C, and washed with PBS at room temperature. The sections were then covered with 5% normal goat serum for 60 min and incubated with anti-Bmal1, Per1, Per2 or Cry1 antibodies at a dilution of 1:50-1:250 for 1 h, washed with PBS for 10 min three times; the sections were incubated with Alexa-conjugated secondary antibodies 1:200 for 1 h and then washed with PBS.…”

Section: Determination Of Mtp Activitymentioning

confidence: 99%

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Clock is important for food and circadian regulation of macronutrient absorption in mice

Pan

Hussain

2009

Journal of Lipid Research

187

216

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Section: Light-and Food-entrained Regulation Of Clock Genes In the Gusupporting

confidence: 83%

Section: Determination Of Mtp Activitymentioning

confidence: 99%

Clock is important for food and circadian regulation of macronutrient absorption in mice

Pan

Hussain

2009

Journal of Lipid Research

187

216

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“…Transcriptional profiling of the murine distal colon has shown that gene expression in these tissues is circadian: >1,200 genes (3.7% of the mouse genome) exhibit rhythmic expression profiles (8). There are also strong biological rhythms in gene expression of the immune system of the small intestine (7). In addition, a recent study of the brush border of healthy mammalian enterocytes has reported that these cells periodically bleb their apical surfaces, releasing vesicles into the gastrointestinal lumen (40), although it was not determined whether this release occurs on a diel rhythm.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have begun to find evidence of strong diel rhythms in gene expression and immune response by the cells of the mammalian gut (7,8). These rhythms are likely to impact the intestinal microbiota and, thereby, their role in normal tissue development, efficient nutrition, and disease resistance (3); nevertheless, little is known about how the microbiota either respond to, or control, these rhythms.…”

mentioning

confidence: 99%

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Wier¹,

Nyholm

Mandel³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

152

221

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Mechanisms for controlling symbiont populations are critical for maintaining the associations that exist between a host and its microbial partners. We describe here the transcriptional, metabolic, and ultrastructural characteristics of a diel rhythm that occurs in the symbiosis between the squid Euprymna scolopes and the luminous bacterium Vibrio fischeri. The rhythm is driven by the host's expulsion from its light-emitting organ of most of the symbiont population each day at dawn. The transcriptomes of both the host epithelium that supports the symbionts and the symbiont population itself were characterized and compared at four times over this daily cycle. The greatest fluctuation in gene expression of both partners occurred as the day began. Most notable was an up-regulation in the host of >50 cytoskeleton-related genes just before dawn and their subsequent down-regulation within 6 h. Examination of the epithelium by TEM revealed a corresponding restructuring, characterized by effacement and blebbing of its apical surface. After the dawn expulsion, the epithelium reestablished its polarity, and the residual symbionts began growing, repopulating the light organ. Analysis of the symbiont transcriptome suggested that the bacteria respond to the effacement by up-regulating genes associated with anaerobic respiration of glycerol; supporting this finding, lipid analysis of the symbionts' membranes indicated a direct incorporation of host-derived fatty acids. After 12 h, the metabolic signature of the symbiont population shifted to one characteristic of chitin fermentation, which continued until the following dawn. Thus, the persistent maintenance of the squid-vibrio symbiosis is tied to a dynamic diel rhythm that involves both partners.Euprymna scolopes | microarray | mutualism | Vibrio fischeri | cytoskeleton

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“…A growing body of evidence shows that peripheral immune cells harbor functional molecular clocks. Thus, rhythmic expression of clock genes has been described in mouse peritoneal macrophages (4), jejunum (5), and spleen and lymph nodes (6). It also is becoming evident that disruption of daily rhythms affects immune response (7).…”

mentioning

confidence: 99%

Core circadian protein CLOCK is a positive regulator of NF-κB–mediated transcription

Spengler¹,

Kuropatwinski²,

Comas³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

275

253

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The circadian clock controls many physiological parameters including immune response to infectious agents, which is mediated by activation of the transcription factor NF-κB. It is widely accepted that circadian regulation is based on periodic changes in gene expression that are triggered by transcriptional activity of the CLOCK/BMAL1 complex. Through the use of a mouse model system we show that daily variations in the intensity of the NF-κB response to a variety of immunomodulators are mediated by core circadian protein CLOCK, which can up-regulate NF-κB-mediated transcription in the absence of BMAL1; moreover, BMAL1 counteracts the CLOCK-dependent increase in the activation of NF-κB-responsive genes. Consistent with its regulatory function, CLOCK is found in protein complexes with the p65 subunit of NF-κB, and its overexpression correlates with an increase in specific phosphorylated and acetylated transcriptionally active forms of p65. In addition, activation of NF-κB in response to immunostimuli in mouse embryonic fibroblasts and primary hepatocytes isolated from Clock-deficient mice is significantly reduced compared with WT cells, whereas Clock-Δ19 mutation, which reduces the transactivation capacity of CLOCK on E-box-containing circadian promoters, has no effect on the ability of CLOCK to up-regulate NF-κB-responsive promoters. These findings establish a molecular link between two essential determinants of the circadian and immune mechanisms, the transcription factors CLOCK and NF-κB, respectively.

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Circadian oscillation of innate immunity components in mouse small intestine

Cited by 92 publications

References 37 publications

Clock is important for food and circadian regulation of macronutrient absorption in mice

Clock is important for food and circadian regulation of macronutrient absorption in mice

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Core circadian protein CLOCK is a positive regulator of NF-κB–mediated transcription

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