Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures

Míková, Hana; Kuchtiak, Viktor; Svobodová, Iveta; Spišská, Veronika; Pačesová, Dominika; Balík, Aleš; Bendová, Zdeňka

doi:10.1007/s12035-020-02141-8

Cited by 7 publications

(3 citation statements)

References 60 publications

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“…When interpreting these data, it is usually assumed that on average only ∼ 1% of GluA2 RNA in the adult brain is unedited and therefore has little signi cance. However, studies have suggested that the percentage of Q/R editing varies between parts of the brain, from 7% in the prefrontal cortex (Akbarian et al 1995) to complete editing in the gerbil hippocampus (Rump et al 1996), which is similar to the tissuespeci c editing ratio of the R/G editing site observed in our previous study (Míková et al 2021). Moreover, Q/R editing not only controls Ca 2+ permeability but also regulates the proportion of GluA2-containing surface receptors (Greger et al 2003) and attributes inward rectifying current to the AMPA receptor channel (Bowie and Mayer 1995;Swanson et al 1997), and AMPA receptors with unedited GluA2 are transiently incorporated at silent synapses during long-term potentiation (Morita et al 2014).…”

Section: Discussionsupporting

confidence: 88%

“…The rhythmicity of the Cry2 expression outside the SCN was not always consistent across experiments, and sometimes the expression pro le is unrhythmic or has low amplitude compared to other clock genes (Miyamoto and Sancar 1999;Santos et al 2015). With respect to Gria2, we have shown using in situ hybridization a circadian rhythm of Gria2 expression in the rat SCN and, using direct sequencing of PCR amplicons, a rhythm of Gria2 R/G editing in the rat hippocampus (Míková et al, 2021), but no rhythmicity of Gria2 expression has been demonstrated in the mouse brain. This nding also aligns with our RNA-seq analysis showing no Gria2 different expression between CT5 and CT15 in Adar2 -/and Gria2 R/R mice (Lebedeva 2023, under review).…”

Section: Discussionmentioning

confidence: 75%

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The disturbance of circadian rhythmicity of clock gene expression in Gria2R/R mice; the comparison with C57BL/6J and Adar2-/- mice strains

Lebedeva¹,

Kubištová

Spišská

et al. 2023

Preprint

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Adar2-/- mice are a widely used model to study the physiological consequences of reduced RNA editing. These mice are viable only when the Q/R editing site of the Gria2 subunit of the AMPA receptor is constitutively mutated to the codon for arginine, and Gria2R/R mice often serve as the sole control for Adar2-/- mice. The aim of our study was to investigate whether ADAR2 inactivity and the Gria2R/R phenotype affect the rhythmicity of the pattern of circadian clock genes and the expression of Gria1 and Gria2 subunits in the suprachiasmatic nucleus (SCN), hippocampus, parietal cortex and liver. Our data show that Gria2R/R mice completely lost circadian rhythmicity in the hippocampus compared to Adar2-/- mice. Compared to C57BL/6J mice, expression profiles in hippocampus and parietal cortex of Gria2R/R mice differ to the same extent as in Adar2-/-. These data suggest that the natural gradual increase in the editing of the Q/R site of the Gria2 subunit postnatally may be important for the development of circadian clockwork in some brain structures, and the use of Gria2R/R mice as the only control to Adar2-/- mice in the hippocampus- and parietal cortex- dependent experiments should therefore be considered.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 75%

The disturbance of circadian rhythmicity of clock gene expression in Gria2R/R mice; the comparison with C57BL/6J and Adar2-/- mice strains

Lebedeva¹,

Kubištová

Spišská

et al. 2023

Preprint

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“…Based on the above research in vivo, we found that biological rhythm regulates the process of angiogenesis after ischemic stroke and affects pathological process to a certain extent. As the core transcriptional factor, Bmal1 regulates the circadian pattern of downstream gene expression [49]. Besides this, Bmal1 has been shown to promote angiogenesis in ischemia-injured HUVECs (human umbilical vein endothelial cells) [50].…”

Section: Discussionmentioning

confidence: 99%

The Circadian Clock is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke

Zhang

Zhao

Guo

et al. 2022

Preprint

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Ischemic stroke is a major public health problem in China. Angiogenesis plays an essential role in the rehabilitation of ischemic brain injury. Recent studies demonstrated that the circadian clock is involved in the process of ischemic stroke, however, the exact mechanism of the circadian clock in regulating angiogenesis after cerebral infarction remains unclear. In the present study, we determined that environmental circadian disruption (ECD) could increase the stroke severity and impair the ability of angiogenesis in a transient middle cerebral artery occlusion (MCAO) rat model, by detecting the rat’s infarct volume, neurological test, and western blot of angiogenesis-related protein, including vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2). We further report that the core circadian clock, aryl hydrocarbon receptor nuclear translocator-like 1 (BMAL1, also known as ARNTL1), plays an irreplaceable role in angiogenesis. Overexpression of BMAL1 promotes tube forming, migration, and wound healing capacity, and upregulate the VEGF and Notch pathway protein level. While this kind of promoting effect is reversed by the Notch pathway inhibitor, DAPT, according to the results of angiogenesis capacity and VEGF pathway protein level. In conclusion, our study reveals the intervention of ECD in angiogenesis in ischemic stroke and further identifies the exact mechanism by which BMAL1 regulates angiogenesis through the VEGF-Notch1 pathway.

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The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke

et al. 2023

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Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures

Cited by 7 publications

References 60 publications

The disturbance of circadian rhythmicity of clock gene expression in Gria2R/R mice; the comparison with C57BL/6J and Adar2-/- mice strains

The disturbance of circadian rhythmicity of clock gene expression in Gria2R/R mice; the comparison with C57BL/6J and Adar2-/- mice strains

The Circadian Clock is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke

The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke

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