CircAKT3 inhibits glycolysis balance in lung cancer cells by regulating miR-516b-5p/STAT3 to inhibit cisplatin sensitivity

Xu, Yangling; Jiang, Tiantian; Wu, Changgang; Zhang, Yongqing

doi:10.1007/s10529-020-02846-9

Cited by 64 publications

(37 citation statements)

References 29 publications

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“…CircRNAs are also involved in tumor progression and chemoresistance in diverse cancers [ 12 ]. For instance, circRNA AKT3 impeded cisplatin chemosensitivity via regulating glycolysis balance through modulation of the miR-516b-5p/STAT3 axis in lung cancer cells [ 13 ]. Circular RNA hsa_circ_0000735 (hsa_circ_0000735) is derived from the P2RX1 gene (exons 2–8).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7

et al. 2020

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Background One of the main reasons for the failure of prostate cancer (PCa) treatment is the generation of chemoresistance. Circular RNA hsa_circ_0000735 (hsa_circ_0000735) is connected with the progression of cancer. Nevertheless, the role and regulatory mechanism of hsa_circ_0000735 in the resistance of PCa to docetaxel (DTX) are unclear. Methods Expression levels of hsa_circ_0000735 and miR-7-5p (miR-7) in tissue samples and cells were examined via quantitative real-time polymerase chain reaction (qRT-PCR). The DTX sensitivity, viability, colony formation, cell cycle progression, and apoptosis of DTX-resistant PCa cells were determined via Cell Counting Kit-8 (CCK-8), cell colony formation, or flow cytometry assays. The levels of multidrug resistance protein 1 (MDR1) protein, cyclinD1, and B cell lymphoma 2 (bcl-2) were detected by western blotting. The interaction between hsa_circ_0000735 and miR-7 was verified via dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA pull-down assays. The role of hsa_circ_0000735 in vivo was validated through tumor formation experiments. Results Hsa_circ_0000735 was upregulated and miR-7 was downregulated in DTX-resistant PCa tissues and cells. High hsa_circ_0000735 expression had a shorter overall survival. Both hsa_circ_0000735 knockdown and miR-7 mimic boosted DTX sensitivity, constrained viability, colony formation, cell cycle progression, and fostered apoptosis of DTX-resistant PCa cells. Also, hsa_circ_0000735 silencing elevated DTX sensitivity and repressed tumor growth in PCa in vivo. Mechanistically, hsa_circ_0000735 served as a sponge for miR-7. MiR-7 inhibition overturned hsa_circ_0000735 silencing-mediated impacts on DTX sensitivity and the malignant behaviors of DTX-resistant PCa cells. Conclusion Hsa_circ_0000735 downregulation boosted PCa sensitivity to DTX and reduced tumor growth via sponging miR-7, providing a promising prognostic biomarker and therapeutic target for PCa.

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Section: Introductionmentioning

confidence: 99%

Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7

et al. 2020

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“…In ovarian cancer, Cdr1as was downregulated in cisplatin-resistant tissue and directly interacted with miR-1270 to increase SCAI [172]. CircAKT3 has also been associated with cisplatin resistance in lung cancer via miR-516b-5p/STAT3 [173] and in gastric cancer via miR-198/PIK3R1 [174]. CircPGC also increases cisplatin resistance via the STAT3 pathway in NSCLC by sponging miR-296-5p [175].…”

Section: Chemotherapymentioning

confidence: 99%

The Potential of Circular RNAs as Cancer Biomarkers

Brown

Chinnaiyan

2020

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Circular RNA (circRNA) is a covalently closed RNA structure that has several proposed functions related to cancer development. Recently, cancer-specific and tissue-specific circRNAs have been identified by high throughput sequencing and are curated in publicly available databases. CircRNAs have features that are ideal properties of biomarkers, including conservation, abundance, and stability in plasma, saliva, and urine. Many circRNAs with predictive and prognostic significance in cancer have been described, and functional mechanisms for some circRNAs have been suggested. CircRNA also has great potential as a non-invasive biomarker for early cancer detection, although further investigation is necessary before clinical application is feasible.

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“…Two different works have recently been published describing circRNAs that regulate the expression of STAT3. Dong et al [48] reported that upregulation of hsa_circ_0076305 confers DDP-resistance to NSCLC cells via sponging miR-296-5p, positively modulating STAT3. Xu et al [49] introduced the role of circAKT3 inhibiting cisplatin sensitivity by regulating mir-516b-5p/STAT3 axis.…”

Section: Circrnas As Biomarkers Of Treatment Resistance In Nsclcmentioning

confidence: 99%

Defining the landscape of circRNAs in non-small cell lung cancer and their potential as liquid biopsy biomarkers: a complete review including current methods

Pedraz-Valdunciel¹,

Rosell²

2021

EVCNA

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Despite the significant decrease in population-level mortality of lung cancer patients as reflected in the Surveillance Epidemiology and End Results program national database, lung cancer, with non-small cell lung cancer (NSCLC) in the lead, continues to be the most commonly diagnosed cancer and foremost cause of cancer-related death worldwide, primarily due to late-stage diagnosis and ineffective treatment regimens. Although innovative single therapies and their combinations are constantly being tested in clinical trials, the five-year survival rate of latestage lung cancer remains only 5% (Cancer Research, UK). Henceforth, investigation in the early diagnosis of lung cancer and prediction of treatment response is critical for improving the overall survival of these patients. Circular RNAs (circRNAs) are a re-discovered type of RNAs featuring stable structure and high tissue-specific expression. Evidence has revealed that aberrant circRNA expression plays an important role in carcinogenesis and tumor progression. Further investigation is warranted to assess the value of EV-and platelet-derived circRNAs as liquid biopsy-based readouts for lung cancer detection. This review discusses the origin and biology of circRNAs, and analyzes their present landscape in NSCLC, focusing on liquid biopsies to illustrate the different methodological

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CircAKT3 inhibits glycolysis balance in lung cancer cells by regulating miR-516b-5p/STAT3 to inhibit cisplatin sensitivity

Cited by 64 publications

References 29 publications

Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7

Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7

The Potential of Circular RNAs as Cancer Biomarkers

Defining the landscape of circRNAs in non-small cell lung cancer and their potential as liquid biopsy biomarkers: a complete review including current methods

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