Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus

Menéndez-Helman, Renata J.; Ferreyroa, Gisele V.; Afonso, Marı́a dos Santos; Salibián, Alfredo

doi:10.1016/j.ecoenv.2014.10.017

Cited by 20 publications

(9 citation statements)

References 40 publications

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“…In our study, AChE activity was measured in summer, when the mean ambient temperature was 26.5 °C, whereas in Pytasz et al (1977) study it was measured in frogs maintained at 15-18 °C. As the highest AChE activity was observed in summer, decreasing significantly below 45% in winter in freshwater fish Cnesterodon decemmaculatus (Menéndez-Helman et al, 2015), we cannot exclude the possibility that the difference in AChE activity in bullfrog muscles between our findings and those reported by Pytasz et al (1977) may be related to seasonal changes in muscle activity.…”

Section: Discussioncontrasting

confidence: 55%

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

et al. 2017

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Sciatic nerve transection (SNT), a model for studying neuropathic pain, mimics the clinical symptoms of "phantom limb", a pain condition that arises in humans after amputation or transverse spinal lesions. In some vertebrate tissues, this condition decreases acetylcholinesterase (AChE) activity, the enzyme responsible for fast hydrolysis of released acetylcholine in cholinergic synapses. In spinal cord of frog Rana pipiens, this enzyme's activity was not significantly changed in the first days following ventral root transection, another model for studying neuropathic pain. An answerable question is whether SNT decreases AChE activity in spinal cord of frog Lithobates catesbeianus, a species that has been used as a model for studying SNT-induced neuropathic pain. Since each animal model has been created with a specific methodology, and the findings tend to vary widely with slight changes in the method used to induce pain, our study assessed AChE activity 3 and 10 days after complete SNT in lumbosacral spinal cord of adult male bullfrog Lithobates catesbeianus. Because there are time scale differences of motor endplate maturation in rat skeletal muscles, our study also measured the AChE activity in bullfrog tibial posticus (a postural muscle) and gastrocnemius (a typical skeletal muscle that is frequently used to study the motor system) muscles. AChE activity did not show significant changes 3 and 10 days following SNT in spinal cord. Also, no significant change occurred in AChE activity in tibial posticus and gastrocnemius muscles at day 3. However, a significant decrease was found at day 10, with reductions of 18% and 20% in tibial posticus and gastrocnemius, respectively. At present we cannot explain this change in AChE activity. While temporally different, the direction of the change was similar to that described for rats. This similarity indicates that bullfrog is a valid model for investigating AChE activity following SNT.Keywords: acetylcholinesterase, sciatic nerve transection, tibial posticus, gastrocnemius, spinal cord, frog. Efeito da Secção do Nervo Isquiático sobre a atividade da acetilcolinesterase em medula espinal e músculos esqueléticos da rã-touro Lithobates catesbeianus ResumoA transecção do nervo isquiático (SNT), um modelo para estudar dor neuropática, simula os sintomas clínicos do "membro fantasma", uma condição dolorosa que ocorre nos humanos após amputação ou secção completa da medula espinal. Essa condição muda a atividade da acetilcolinesterase (AChE), a enzima responsável pela rápida hidrólise da acetilcolina liberada nas sinapses colinérgicas, em alguns tecidos de vertebrados. Em medula espinal de rã Rana pipiens, a atividade da AChE não foi significativamente alterada nos primeiros dias após a secção da raiz ventral, outro modelo para o estudo da dor neuropática. Uma questão ainda não respondida é se a SNT diminui a atividade da AChE na medula espinal de rã Lithobates catesbeianus, uma espécie que vem sendo usada como modelo em estudos da dor neuropática induzida por SNT. Como...

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Section: Discussioncontrasting

confidence: 55%

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

et al. 2017

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“…Since then, aquatic ecologists have used the standard dose-response monitoring approach for detection of molecular-level damages in aquatic organisms [13][14]. In addition to this novel approach, change in physiological responses such as acetylcholinesterase (AChE) activity [15][16] and 7-ethoxyresorufin-O-deethylase (EROD) activity [17] estimation could be used as an excellent barometer (biomarker) in detecting the cause-effect problems at the cellular or sub-cellular levels. Therefore, due to the above-mentioned reasons, such approaches can be considered as integral parts of an early warning (alarm) system, which can provide significantly reliable clues regarding the imminent threats and existing issues in aquatic ecosystem health [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Bae¹,

Atique²,

Yoon³

et al. 2020

Pol. J. Environ. Stud.

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Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T L), and tail extent moment (TEM) in impacted zone (I z) were 2.0-3.6-fold greater than in controls (C z). Nucleus image analysis showed that the nucleus had circular particle forms in the C z as compared with a longitudinal oval form with broken particles from the nucleus in the I z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I z than in the C z. Such DNA damages and greater physiological responses in the I z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.

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“…The use of enzymatic activity as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in biomonitoring protocols, requires the understanding of the toxic-biological response relationship, the natural fluctuation of a particular biomarker and the validation in the system under study. Previous reports indicated that basal levels of AChE activity can also be affected by fish length and seasonal variability (Beauvais et al, 2002;Chuiko et al, 1997;Menéndez-Helman et al, 2015). In this regard, the response to a stress factor cannot be assumed to be always the same.…”

Section: Introductionmentioning

confidence: 99%

Seasonal variations in the dose-response relationship of acetylcholinesterase activity in freshwater fish exposed to chlorpyrifos and glyphosate

Bernal-Rey

Cantera

Menéndez-Helman

2020

Ecotoxicology and Environmental Safety

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Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus

Cited by 20 publications

References 40 publications

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Seasonal variations in the dose-response relationship of acetylcholinesterase activity in freshwater fish exposed to chlorpyrifos and glyphosate

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