CircHN1 affects cell proliferation and migration in gastric cancer

Zhang, Yu; Wang, Maoye; Zang, Xueyan; Mao, Zheying; Chen, Yanke; Mao, Fei; Qian, Hui; Xu, Wenrong; Xu, Zu-De

doi:10.1002/jcla.23433

Cited by 19 publications

(17 citation statements)

References 35 publications

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“… 27 However, Zhang et al reported that miR-1248 functioned a tumor suppressor in gastric cancer. 28 Therefore, miR-1248 might have different roles in different cancers. However, it was worth noting that miR-1248 also was significantly low in NSCLC, 29 and our findings were consistent with it.…”

Section: Discussionmentioning

confidence: 99%

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

Yue¹,

Xu²,

Duan³

et al. 2021

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Background Circular RNA (circRNA) is a key regulator of cancer, and it has been proved to be involved in the regulation of cancer progression including non-small cell lung cancer (NSCLC). Circ-PITX1 was found to be a significantly upregulated circRNA in NSCLC, and its role and potential mechanism in NSCLC progression deserve further investigation. Methods The expression levels of circ-PITX1, microRNA (miR)-1248 and cyclin D2 (CCND2) were examined by quantitative real-time PCR (qRT-PCR). Cell proliferation, apoptosis, cell cycle process, migration and invasion were determined using cell counting kit 8 (CCK8) assay, colony formation assay, flow cytometry, wound healing assay and transwell assay. Xenograft models were built to explore the role of circ-PITX1 in NSCLC tumor growth in vivo. The glycolysis and glutamine metabolism of cells were assessed by detecting the consumptions of glucose and glutamine, cell extracellular acidification rate (ECAR), and the productions of lactate, α-ketoglutaric acid (α-KG) and ATP. The protein levels of hexokinase 2 (HK-2), glutaminase 1 (GLS1) and CCND2 were tested by Western blot (WB) analysis. Dual-luciferase reporter assay and RIP assay were employed to verify the interaction between miR-1248 and circ-PITX1 or CCND2. Results Circ-PITX1 was upregulated in NSCLC and its silencing could inhibit the proliferation, migration, invasion, cell cycle process, glycolysis, glutamine metabolism, and promote the apoptosis of NSCLC cells in vitro, as well as reduced tumor growth in vivo. In the terms of mechanism, we found that circ-PITX1 could act as a sponge of miR-1248, and miR-1248 could target CCND2. In addition, miR-1248 inhibitor reversed the inhibitory effect of circ-PITX1 knockdown on NSCLC progression. Similarly, CCND2 overexpression also reversed the suppressive effect of miR-1248 on NSCLC progression. Moreover, circ-PITX1 positively regulated CCND2 expression by sponging miR-1248. Conclusion Circ-PITX1 served as a sponge of miR-1248 to promote NSCLC progression by upregulating CCND2.

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Section: Discussionmentioning

confidence: 99%

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

Yue¹,

Xu²,

Duan³

et al. 2021

OTT

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“…Recent studies found that circRNAs containing miRNA response elements (MREs) can work as miRNA sponges 14,15 . For example, CiRS‐7 containing 74 miR‐7 binding sites can act as a miR‐7 sponge with high miRNA‐binding capacity and can participate in post‐transcriptional regulation 5 .…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies found that circRNAs containing miRNA response elements (MREs) can work as miRNA sponges. 14,15 For example, CiRS-7 containing 74 miR-7 binding sites can act as a miR-7 sponge with high miRNA-binding capacity and can participate in posttranscriptional regulation. 5 MiR-7 plays a key role in Parkinson's disease, which indicates the potential role of CiRS-7 in the progression of Parkinson's disease.…”

Section: Discussionmentioning

confidence: 99%

Reduced expression of circRNA hsa_circ_001888 in gastric cancer and its clinical significance

Shi

Song

Ding

2021

Clinical Laboratory Analysis

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Background Circular RNAs (circRNAs) are a novel family of endogenous RNAs. Recent studies have demonstrated that circRNAs are potential novel biomarkers for diagnosing cancers. However, little is known about the role of circRNAs in gastric cancer (GC). This study aimed to identify the relationship between GC and a new circRNA named hsa_circ_001888. Methods Hsa_circ_001888 expression levels were measured by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in GC cell lines, tissues, and plasma samples. Then, the associations between the expression level of hsa_circ_001888 and the clinicopathological features of patients with GC were further investigated. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value of hsa_circ_001888. Results In this study, hsa_circ_001888 was first found to be significantly downregulated in GC cell lines (AGS and MKN‐45), tissues, and plasma samples compared to control samples. Clinicopathological features showed that the expression of hsa_circ_001888 in GC tissues was associated with differentiation and in GC plasma linked with serum CEA and CA19‐9 levels. The areas under the ROC curves of hsa_circ_001888 in tissues and plasma were 0.654 and 0.66, respectively. Conclusions Hsa_circ_001888 may serve as a potential biomarker in the diagnosis of GC and may be involved in GC development.

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“… 15 Similarly, circHN1, circMAN2B2, and hsa_circ_0023642 were also found to affect the growth and migration of GC cells by the similar ways. 16 , 17 , 18 All these circRNAs can serve as potential biomarkers and targets for the diagnosis and therapy of GC, which strongly suggests an important role of altered circRNAs in cancer pathophysiology.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0001874 is an indicator for gastric cancer

et al. 2021

Clinical Laboratory Analysis

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Background Recent studies have indicated that circular RNAs (circRNAs) are novel endogenous RNAs whose 5′ and 3′ ends are covalently linked and play critical roles in gastric carcinogenesis. However, the significance of circRNA hsa_circ_0001874 in gastric cancer (GC) is still unclear. Methods Therefore, we first detected hsa_circ_0001874 levels in GC cell lines and tissues and analyzed their potential correlation with clinicopathological factors. Then, a receiver operating characteristic (ROC) curve was established to evaluate its clinical value. Finally, we further predicted the biological functions of this molecule by bioinformatics analysis. Results Our data showed that as an indicator, hsa_circ_0001874 expression was significantly decreased in 78.02% (71/91) of the GC patients. Combined with clinicopathological factors, the hsa_circ_0001874 level was strongly associated with cell differentiation (p < 0.001), tumor stage (p = 0.005), invasion (p = 0.024), lymphatic metastasis (p = 0.023), and CEA level (p < 0.001) in GC tissues. The area under the curve (AUC) was up to 0.673, with a sensitivity and specificity of 61.54% and 68.13%, respectively. Bioinformatics analysis showed that hsa_circ_0001874 harbors miR‐593‐5p, miR‐103a‐3p, and miR‐107 seed sequences to regulate these three miRNAs and downstream target genes and exert its various biological functions in the carcinogenesis and progression of GC. Conclusion In summary, these data suggest that hsa_circ_0001874 is an indicator of GC and plays a significant role in gastric carcinogenesis and progression.

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CircHN1 affects cell proliferation and migration in gastric cancer

Cited by 19 publications

References 35 publications

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

Reduced expression of circRNA hsa_circ_001888 in gastric cancer and its clinical significance

Circular RNA hsa_circ_0001874 is an indicator for gastric cancer

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