Reduced expression of circRNA hsa_circ_001888 in gastric cancer and its clinical significance

Abstract

Background Circular RNAs (circRNAs) are a novel family of endogenous RNAs. Recent studies have demonstrated that circRNAs are potential novel biomarkers for diagnosing cancers. However, little is known about the role of circRNAs in gastric cancer (GC). This study aimed to identify the relationship between GC and a new circRNA named hsa_circ_001888. Methods Hsa_circ_001888 expression levels were measured by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in GC cell lines, tissues, and plasma samples. Then, the associations between the expression level of hsa_circ_001888 and the clinicopathological features of patients with GC were further investigated. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value of hsa_circ_001888. Results In this study, hsa_circ_001888 was first found to be significantly downregulated in GC cell lines (AGS and MKN‐45), tissues, and plasma samples compared to control samples. Clinicopathological features showed that the expression of hsa_circ_001888 in GC tissues was associated with differentiation and in GC plasma linked with serum CEA and CA19‐9 levels. The areas under the ROC curves of hsa_circ_001888 in tissues and plasma were 0.654 and 0.66, respectively. Conclusions Hsa_circ_001888 may serve as a potential biomarker in the diagnosis of GC and may be involved in GC development.

The diagnostic significance of blood-derived circRNAs in NSCLC: Systematic review and meta-analysis

“…It also showed a significant effect in AFP-negative hepatocellular carcinoma. Shi et al (41) similarly found that low expression of plasmaderived hsa_circ_001888 in gastric cancer may be a potential diagnostic marker.…”

“…Shi et al. ( 41 ) similarly found that low expression of plasma-derived hsa_circ_001888 in gastric cancer may be a potential diagnostic marker.…”

Circ_0010235 Regulates HOXA10 Expression to Promote Malignant Phenotypes and Radioresistance in Non-small Cell Lung Cancer Cells Via Decoying miR-588

“…CircRNAs have high structural stability and tissue specificity and these characteristics provide the important foundation for circRNAs as cancer biomarkers 18 . For example, circ_001888 was abnormally expressed in gastric cancer and used as a probable diagnostic biomarker 19 . Circ_0010235 was identified as a closed-loop circRNA through the stability analysis using RNase R treatment and through the structure analysis using Oligo (dT) 18 primers.…”

Detecting plasma hsa_circ_0061276 in patients with gastric cancer by reverse transcription-digital polymerase chain reaction

“…Since circRNAs covalently bind the 5' and 3' ends of RNAs into a circular structure (21), circRNAs are considered to be more stable than miRNAs, mRNAs, and long noncoding RNAs (22). Given that circRNAs are widely present in body fluids, including plasma, serum, exosomes, and urine, they are a new biomarkers for monitoring cancer development and progression (9,10,23,24). To clarify the relationship between the abnormal expression of circRNAs and their prognostic value in gastric cancer patients, Chen et al searched articles reporting the relationship between circRNAs and the prognosis of gastric cancer through a meta-analysis (25).…”

“…Their unique circular structure results in their high stability. circRNAs commonly exist in various human body fluids and exosomes, offering the potential of using circRNAs as biomarkers of gastric cancer (9,10). Many researchers have elucidated the multiple functions of circRNAs, such as competitively binding to proteins and microRNAs (miRNAs), participating in protein coding, and regulating gene transcription (11)(12)(13)(14).…”