CircRNA circ_0067934 promotes tumor growth and metastasis in hepatocellular carcinoma through regulation of miR-1324/FZD5/Wnt/β-catenin axis

Zhu, Qian; Lu, Guiyu; Luo, Zihua; Gui, Fenfang; Wu, Jinghua; Zhang, Dongwei; Ni, Yicheng

doi:10.1016/j.bbrc.2018.02.119

Cited by 170 publications

(140 citation statements)

References 26 publications

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“…Elsewhere, it was found that hsa_circ_0000673 enhanced HCC severity via by modulating the miR‐767‐3p/SET pathway. A previous study showed that hsa_circ_0067934 can increase invasion, migration, and proliferation of HCC cells . Mechanistically, hsa_circ_0067934 inhibits the activity of miR‐1324 thereby activating frizzled class receptor 5 (FZD5)/Wnt/ β ‐catenin signaling pathway.…”

Section: Oncogenic Functions Of Circrnas In Hccmentioning

confidence: 99%

“…A previous study showed that hsa_circ_0067934 can increase invasion, migration, and proliferation of HCC cells. 66 Mechanistically, hsa_circ_0067934 inhibits the activity of miR-1324 thereby activating frizzled class receptor 5 (FZD5)/Wnt/β-catenin signaling pathway. Taken together, these findings show that hsa_circ_0067934 promotes HCC progression and can be exploited for HCC treatment.…”

Section: Circrnas In Hccmentioning

confidence: 99%

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Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

Shang

Adzika

et al. 2019

Cancer Medicine

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At the heart of hepatocellular carcinoma (HCC) lies disruption of signaling pathways at the level of molecules, genes, and cells. Non‐coding RNAs (ncRNAs) have been implicated in the disease progression of HCC. For instance, dysregulated expression of circular RNAs (circRNAs) has been observed in patients with HCC. As such, these RNAs are potential therapeutic targets and diagnostic markers for HCC. Long non‐coding RNAs (lncRNAs), a type of ncRNA, have also been recognized to participate in the initiation and progression of HCC. Transforming growth factor‐beta (TGF‐β) is another element which is now recognized to play crucial roles in HCC. It has been implicated in many biological processes such as survival, immune surveillance, and cell proliferation. In HCC, TGF‐β promotes disease progression by two mechanisms: an intrinsic signaling pathway and the extrinsic pathway. Through these pathways, it modulates various microenvironment factors such as inflammatory mediators and fibroblasts. An interesting yet‐to‐be resolved concept is whether the HCC‐promoting role of TGF‐β pathways is limited to a subset of HCC patients or it is involved in the whole process of HCC development. This review summarizes recent advancements to highlight the roles of circRNAs, lncRNAs, and TGF‐β in HCC.

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Section: Oncogenic Functions Of Circrnas In Hccmentioning

confidence: 99%

Section: Circrnas In Hccmentioning

confidence: 99%

Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

Shang

Adzika

et al. 2019

Cancer Medicine

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“…Salmena et al [41] proposed the "ceRNA" hypothesis that states that any RNA containing miRNA response elements, such as mRNA, pseudogenes, lncRNA and circRNA, can competitively bind to miRNA. Since then, this hypothesis has been proven by increasing bioinformatic and experimental 1 9 evidence [27,29,54,55]. For example, by performing anti-AGO2 RNA precipitation and luciferase reporter assays, Ma and colleagues found that circRNA-000284 was closely associated with miR-506 in cervical cancer cells; they also found that the tumor-promoting effect of circRNA-000284 could be abolished by the coexpression of miR-506 mimics or a Snail-2 silencing vector [54].…”

Section: Discussionmentioning

confidence: 99%

“…For example, by performing anti-AGO2 RNA precipitation and luciferase reporter assays, Ma and colleagues found that circRNA-000284 was closely associated with miR-506 in cervical cancer cells; they also found that the tumor-promoting effect of circRNA-000284 could be abolished by the coexpression of miR-506 mimics or a Snail-2 silencing vector [54]. Recently, by conducting rescue experiments, Zhu et al [55] observed that circ_0067934 can enhance the proliferation, downregulation) might contribute to NSCLC cell metastasis [59]. In this work, miR-149-y is highly homologous to miR-149, and we speculated that…”

Section: Discussionmentioning

confidence: 99%

Systematic identification of circular RNAs and corresponding regulatory networks unveil their potential roles in the midgut ofApis cerana ceranaworkers

Chen

et al. 2019

Preprint

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Background: Circular RNAs (circRNAs) are newly discovered noncoding RNAs (ncRNAs) that play key roles in various biological functions, such as the regulation of gene expression and alternative splicing. CircRNAs have been identified in some species, including western honeybees. However, the understanding of honeybee circRNA is still very limited, and to date, no study on eastern honeybee circRNA has been conducted. Here, the circRNAs in the midguts of Apis cerana cerana workers were identified and validated, and the regulatory networks were constructed.Differentially expressed circRNAs (DEcircRNAs) and the corresponding competitively endogenous RNA (ceRNA) networks in the development of the worker's midgut were further investigated.Results: Here, 7-and 10-day-old A. c. cerana workers' midguts (Ac1 and Ac2) were sequenced using RNA-seq, and a total of 9589 circRNAs were predicted using bioinformatics. These circRNAs were approximately 201-800 nt in length and could be classified into six types; the annotated exonic circRNAs were the most abundant.Additionally, five novel A. c. cerana circRNAs were confirmed by PCR amplification and Sanger sequencing, indicating the authenticity of A. c. cerana circRNAs.Interestingly, novel_circ_003723, novel_circ_002714, novel_circ_002451 and novel_circ_001980 were the most highly expressed circRNAs in both Ac1 and Ac2, which is indicative of their key roles in the development of the midgut. Moreover, 55DEcircRNAs were identified in the Ac1 vs Ac2 comparison group, including 34 upregulated and 21 downregulated circRNAs. Further investigation showed that the 3 source genes of circRNAs were classified into 34 GO terms and were involved in 141 KEGG pathways. In addition, the source genes of DEcircRNAs were categorized into 10 GO terms and 15 KEGG pathways, which demonstrated that the corresponding DEcircRNAs may affect the growth, development, and material and energy metabolisms of the worker's midgut by regulating the expression of the related source genes. Additionally, the circRNA-miRNA regulatory networks were constructed and analyzed, and the results demonstrated that 1060 circRNAs can bind to 74 miRNAs and that 71.51% of circRNAs can be linked to only one miRNA. Furthermore, the DEcircRNA-miRNA-mRNA networks were constructed and explored, and the results indicate that the 13 downregulated circRNAs can bind to eight miRNAs and to 29 target genes. In addition, the results indicate that the 16 upregulated circRNAs can bind to 9 miRNAs and to 29 target genes, demonstrating that DEcircRNAs are likely involved in the regulation of midgut development via ceRNA mechanisms. Moreover, the regulatory networks of miR-6001-y-targeted DEcircRNAs were analyzed, and the results showed that eight DEcircRNAs may affect the development of A. c. cerana workers' midguts by targeting miR-6001-y. Finally, four randomly selected DEcircRNAs were verified via RT-qPCR, confirming the reliability of our sequencing data.Conclusion: This is the first systematic investigation of circRNAs and their correspond...

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“…In recent years, increasing circRNAs have been identified in mammalian cells by using advanced sequencing technology. CircRNAs possess powerful regulatory functions affecting all varieties of biological processes and are potential diagnostic markers and therapeutic targets for cancer . Recent studies reported that some circRNAs function as tumor suppressors or oncogenes in gastric cancer, bladder cancer, pancreatic cancer, hepatocellular carcinoma and other types of cancer.…”

Section: Discussionmentioning

confidence: 99%

CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

2019

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The circRNA circAGFG1 is reported to be important in triple‐negative breast cancer progression. However, the mechanism of circAGFG1 in non‐small‐cell lung cancer (NSCLC) remains unknown. In this study, expression of circAGFG1 was determined by real‐time PCR in 20 pairs of NSCLC tissues and adjacent tissues. Next, functional experiments with circAGFG1 were performed in vitro to evaluate the role of circAGFG1 in tumor metastasis and growth. Meanwhile, a dual luciferase reporter assay, RNA pull‐down and RNA immunoprecipitation experiments were used to explore the interaction between circAGFG1 and miR‐203. Our results revealed that expression levels of circAGFG1 and miR‐203 are upregulated in non‐small‐cell lung cancer tissues. CircAGFG1 enhances NSCLC cell proliferation, invasion, migration and epithelial‐mesenchymal transition in vitro. Mechanistic analyses indicated that circAGFG1 acts as a sponge for miR‐203 to repress the effect of miR‐203 on its target, ZNF281. In conclusion, our study suggests that circAGFG1 promotes NSCLC growth and metastasis though a circAGFG1/miR‐203/ZNF281 axis and may represent a novel therapeutic target.

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CircRNA circ_0067934 promotes tumor growth and metastasis in hepatocellular carcinoma through regulation of miR-1324/FZD5/Wnt/β-catenin axis

Cited by 170 publications

References 26 publications

Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

Systematic identification of circular RNAs and corresponding regulatory networks unveil their potential roles in the midgut ofApis cerana ceranaworkers

CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

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